##gff-version 3
##sequence-region BBa_K235034 1 2499
BBa_K235034	.	promoter	1	1422	.	+	0	ID=BBa_K235033;Name=BBa_K235033
BBa_K235034	.	engineered_region	133	1422	.	+	0	ID=BBa_K235031;Name=BBa_K235031;Parent=BBa_K235033
BBa_K235034	.	terminator	1294	1422	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K235031
BBa_K235034	.	terminator	1382	1422	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K235034	.	engineered_region	1382	1422	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235034	.	stop_codon	1415	1415	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235034	.	stem_loop	1389	1408	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235034	.	polyA_site	1409	1422	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235034	.	terminator	1294	1373	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K235034	.	engineered_region	1294	1373	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235034	.	stem_loop	1305	1348	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235034	.	CDS	133	1260	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K235031
BBa_K235034	.	engineered_tag	1261	1285	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K235034	.	engineered_region	133	1260	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K235034	.	CDS	133	1260	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K235034	.	tag	1222	1260	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K235034	.	promoter	1	126	.	+	0	ID=BBa_K235030;Name=BBa_K235030;Parent=BBa_K235033
BBa_K235034	.	mature_transcript_region	44	126	.	+	0	ID=BBa_K115017;Name=BBa_K115017;Parent=BBa_K235030
BBa_K235034	.	ribosome_entry_site	120	125	.	+	0	ID=annotation1972920;Name=SD;Parent=BBa_K115017
BBa_K235034	.	promoter	1	35	.	+	0	ID=BBa_J23102;Name=BBa_J23102;Parent=BBa_K235030
BBa_K235034	.	engineered_region	1431	2499	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K235034	.	terminator	2371	2450	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K235034	.	engineered_region	2371	2450	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235034	.	stem_loop	2382	2425	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235034	.	promoter	1431	1630	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K235034	.	non_covalent_binding_site	1519	1556	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K235034	.	promoter	1591	1596	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K235034	.	engineered_region	1431	1630	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K235034	.	CDS	1431	1518	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K235034	.	non_covalent_binding_site	1596	1630	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K235034	.	promoter	1567	1572	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K235034	.	start_codon	1603	1603	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K235034	.	terminator	2459	2499	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K235034	.	engineered_region	2459	2499	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235034	.	stop_codon	2492	2492	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235034	.	stem_loop	2466	2485	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235034	.	polyA_site	2486	2499	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235034	.	CDS	1657	2337	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K235034	.	engineered_tag	2338	2362	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K235034	.	CDS	1657	2331	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K235034	.	ribosome_entry_site	1639	1650	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K235034	.	conserved_region	1643	1646	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K235034
ttgacagctagctcagtcctaggtactgtgctagctactagagccgggcgcccttcgggggcccggcggagacgggcgc
cggaggtgtccgacgcctgctcgtccagtctttgctcagtggaggattactagatggtgaatgtgaaaccagtaacgtt
atacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcg
aaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggca
aacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatc
tcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtg
cacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaag
ctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatga
agacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagt
tctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacggg
aaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgct
ggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcg
gtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgc
tggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctc
actggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcag
ctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactct
gatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgac
tggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgc
ttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatg
gcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcg
aaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgct
gccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccg
gactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccg
ttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacgg
tccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggt
gaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaac
cggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttga
acagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata