LOCUS       BBa_K235035             2516 bp    DNA     linear       01-Jun-2024
DEFINITION  [K235033][K235032] (constituitive promoter+32??C RBS 
            thermometer+LacI+stop+pLac+RBS+GFP+stop)
ACCESSION   BBa_K235035
VERSION     BBa_K235035.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             120..125
                     /label=SD
     misc_feature    44..126
                     /label=BBa_K115017
     promoter        1..126
                     /label=BBa_K235030
     misc_feature    1222..1260
                     /label=LVA
     CDS             133..1260
                     /label=lacI-LVA
     misc_feature    133..1260
                     /label=BBa_C0012
     misc_feature    1261..1285
                     /label=Help:Barcodes
     CDS             133..1260
                     /label=BBa_C0012
     stem_loop       1305..1348
                     /label=stem_loop
     misc_feature    1294..1373
                     /label=BBa_B0010
     terminator      1294..1373
                     /label=BBa_B0010
     stem_loop       1389..1408
                     /label=T7 TE
     misc_feature    1415..1415
                     /label=stop
     polyA_site      1409..1422
                     /label=polya
     misc_feature    1382..1422
                     /label=BBa_B0012
     terminator      1382..1422
                     /label=BBa_B0012
     terminator      1294..1422
                     /label=BBa_B0015
     misc_feature    133..1422
                     /label=BBa_K235031
     promoter        1..1422
                     /label=BBa_K235033
     CDS             1431..1518
                     /label=end of LacI coding region (inactive)
     misc_feature    1431..1630
                     /label=BBa_R0010
     misc_feature    1519..1556
                     /label=CAP binding site
     promoter        1567..1572
                     /label=-35
     promoter        1591..1596
                     /label=-10
     misc_feature    1596..1630
                     /label=LacI binding site
     misc_feature    1603..1603
                     /label=start
     promoter        1431..1630
                     /label=BBa_R0010
     RBS             1639..1653
                     /label=RBS-1\Strong
     RBS             1646..1650
                     /label=RBS
     misc_feature    1639..1653
                     /label=BBa_B0030
     RBS             1639..1653
                     /label=BBa_B0030
     CDS             1660..2379
                     /label=GFP protein
     CDS             1660..2379
                     /label=BBa_E0040
     stem_loop       2399..2442
                     /label=stem_loop
     misc_feature    2388..2467
                     /label=BBa_B0010
     terminator      2388..2467
                     /label=BBa_B0010
     stem_loop       2483..2502
                     /label=T7 TE
     misc_feature    2509..2509
                     /label=stop
     polyA_site      2503..2516
                     /label=polya
     misc_feature    2476..2516
                     /label=BBa_B0012
     terminator      2476..2516
                     /label=BBa_B0012
     misc_feature    1431..2516
                     /label=BBa_K235032
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagccgggcg cccttcgggg
       61 gcccggcgga gacgggcgcc ggaggtgtcc gacgcctgct cgtccagtct ttgctcagtg
      121 gaggattact agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc
      181 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
      241 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
      301 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
      361 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
      421 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
      481 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
      541 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
      601 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
      661 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
      721 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
      781 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
      841 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
      901 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
      961 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
     1021 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     1081 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     1141 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1201 tcccgactgg aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa
     1261 ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc
     1321 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1381 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag caatacgcaa
     1441 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1501 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     1561 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     1621 atttcacaca tactagagat taaagaggag aaatactaga tgcgtaaagg agaagaactt
     1681 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     1741 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     1801 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
     1861 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
     1921 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     1981 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     2041 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     2101 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     2161 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     2221 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     2281 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     2341 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
     2401 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2461 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//