##gff-version 3
##sequence-region BBa_K235035 1 2516
BBa_K235035	.	engineered_region	1431	2516	.	+	0	ID=BBa_K235032;Name=BBa_K235032
BBa_K235035	.	terminator	2476	2516	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K235032
BBa_K235035	.	engineered_region	2476	2516	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235035	.	polyA_site	2503	2516	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235035	.	stop_codon	2509	2509	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235035	.	stem_loop	2483	2502	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235035	.	terminator	2388	2467	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K235032
BBa_K235035	.	engineered_region	2388	2467	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235035	.	stem_loop	2399	2442	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235035	.	CDS	1660	2379	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K235032
BBa_K235035	.	CDS	1660	2379	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K235035	.	promoter	1431	1630	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K235032
BBa_K235035	.	start_codon	1603	1603	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K235035	.	non_covalent_binding_site	1519	1556	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K235035	.	engineered_region	1431	1630	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K235035	.	promoter	1591	1596	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K235035	.	non_covalent_binding_site	1596	1630	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K235035	.	CDS	1431	1518	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K235035	.	promoter	1567	1572	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K235035	.	ribosome_entry_site	1639	1653	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K235032
BBa_K235035	.	engineered_region	1639	1653	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K235035	.	ribosome_entry_site	1639	1653	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K235035	.	ribosome_entry_site	1646	1650	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K235035	.	promoter	1	1422	.	+	0	ID=BBa_K235033;Name=BBa_K235033
BBa_K235035	.	engineered_region	133	1422	.	+	0	ID=BBa_K235031;Name=BBa_K235031;Parent=BBa_K235033
BBa_K235035	.	CDS	133	1260	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K235031
BBa_K235035	.	engineered_tag	1261	1285	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K235035	.	engineered_region	133	1260	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K235035	.	CDS	133	1260	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K235035	.	tag	1222	1260	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K235035	.	terminator	1294	1422	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K235031
BBa_K235035	.	terminator	1382	1422	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K235035	.	engineered_region	1382	1422	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235035	.	polyA_site	1409	1422	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235035	.	stop_codon	1415	1415	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235035	.	stem_loop	1389	1408	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235035	.	terminator	1294	1373	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K235035	.	engineered_region	1294	1373	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235035	.	stem_loop	1305	1348	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235035	.	promoter	1	126	.	+	0	ID=BBa_K235030;Name=BBa_K235030;Parent=BBa_K235033
BBa_K235035	.	mature_transcript_region	44	126	.	+	0	ID=BBa_K115017;Name=BBa_K115017;Parent=BBa_K235030
BBa_K235035	.	ribosome_entry_site	120	125	.	+	0	ID=annotation1972920;Name=SD;Parent=BBa_K115017
BBa_K235035	.	promoter	1	35	.	+	0	ID=BBa_J23102;Name=BBa_J23102;Parent=BBa_K235030
>BBa_K235035
ttgacagctagctcagtcctaggtactgtgctagctactagagccgggcgcccttcgggggcccggcggagacgggcgc
cggaggtgtccgacgcctgctcgtccagtctttgctcagtggaggattactagatggtgaatgtgaaaccagtaacgtt
atacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcg
aaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggca
aacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatc
tcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtg
cacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaag
ctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatga
agacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagt
tctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacggg
aaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgct
ggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcg
gtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgc
tggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctc
actggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcag
ctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactct
gatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgac
tggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgc
ttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactag
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaat
tttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaact
acctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaa
cagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaact
acaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaaga
agatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaag
aatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaa
atactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcc
caacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatac
aaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata