LOCUS       BBa_K235037              989 bp    DNA     linear       22-Nov-2024
DEFINITION  [K235030][J04630] (constituitive promoter+32??C RBS 
            thermometer+GFP+stop)
ACCESSION   BBa_K235037
VERSION     BBa_K235037.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             120..125
                     /label=SD
     misc_feature    44..126
                     /label=BBa_K115017
     CDS             133..852
                     /label=GFP protein
     CDS             133..852
                     /label=BBa_E0040
     stem_loop       872..915
                     /label=stem_loop
     misc_feature    861..940
                     /label=BBa_B0010
     terminator      861..940
                     /label=BBa_B0010
     stem_loop       956..975
                     /label=T7 TE
     misc_feature    982..982
                     /label=stop
     polyA_site      976..989
                     /label=polya
     misc_feature    949..989
                     /label=BBa_B0012
     terminator      949..989
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagccgggcg cccttcgggg
       61 gcccggcgga gacgggcgcc ggaggtgtcc gacgcctgct cgtccagtct ttgctcagtg
      121 gaggattact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      181 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      241 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      301 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
      361 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      421 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      481 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      541 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      601 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
      661 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      721 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
      781 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
      841 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      901 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      961 accttcgggt gggcctttct gcgtttata
//