LOCUS       BBa_K237002              247 bp    DNA     linear       03-May-2025
DEFINITION  Constitutive RIP Generator. RIP - Quorum sensing inhibitor of 
            Staphylococcus spp.
ACCESSION   BBa_K237002
VERSION     BBa_K237002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=Start
     misc_feature    105..109
                     /label=Stop
     CDS             84..104
                     /label=YSPWTNF
     CDS             81..110
                     /label=BBa_K237000
     stem_loop       130..173
                     /label=stem_loop
     misc_feature    119..198
                     /label=BBa_B0010
     terminator      119..198
                     /label=BBa_B0010
     stem_loop       214..233
                     /label=T7 TE
     misc_feature    240..240
                     /label=stop
     polyA_site      234..247
                     /label=polya
     misc_feature    207..247
                     /label=BBa_B0012
     terminator      207..247
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtattctc cgtggaccaa cttttaataa tactagagcc
      121 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      181 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      241 gtttata
//