BBa_K237002
1
BBa_K237002
Constitutive RIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.
2009-08-02T11:00:00Z
2015-05-08T01:11:36Z
The RIP peptide is produced by coagulase negative staphylococcus (suggested to be S. warnerii or S. xylosus).
RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species. RIP effectively works as an antibiotic in its inhibition of bacterial growth.
RIP has the sequence YSPXTNF and has been tested extensively in a synthetic (as in organic) form for ability to inhibit bacterial infections in mice.
References will come later.
false
false
_333_
0
4087
9
It's complicated
true
There are 4 versions of RIP producers from us.
1 - Constitutively produced RIP without an export sequence
2 - Inducibly produced RIP without an export sequence
3 - Constitutively produced RIP with an export sequence (OmpA)
4 - Inducibly produced RIP with an export sequence (OmpA)
This is number 1
false
Marc T. K. Nielsen
component2014652
1
BBa_R0040
component2014663
1
BBa_B0010
component2014662
1
BBa_K237000
component2014665
1
BBa_B0012
component2014658
1
BBa_B0034
annotation2014658
1
BBa_B0034
range2014658
1
63
74
annotation2014665
1
BBa_B0012
range2014665
1
207
247
annotation2014663
1
BBa_B0010
range2014663
1
119
198
annotation2014652
1
BBa_R0040
range2014652
1
1
54
annotation2014662
1
BBa_K237000
range2014662
1
81
110
BBa_K237000
1
RIP
RIP - Quorum sensing inhibitor of Staphylococcus spp. (Including MRSA)
2009-08-02T11:00:00Z
2015-05-08T01:11:36Z
The RIP peptide is produced by coagulase negative staphylococcus (suggested to be S. warnerii or S. xylosus) (16,19)
RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species. RIP effectively works as an antibiotic in its inhibition of bacterial growth.
RIP has the sequence YSPXTNF and has been tested extensively in a synthetic (as in organic) form for ability to inhibit bacterial infections in mice.
References will come later.
This is a pure RIP Brick, it contains no signal sequence and we can at this point in our project not verify that it will be exported by our Coli's
false
false
_333_
0
4087
9
It's complicated
true
This sequence is reverse-engineered from the amino-acid sequence by using the commonly used codons of E. Coli.
It is designed to provide a big output of peptide. We just hope that this output won't kill the cell.
false
Marc T. K. Nielsen
annotation2014626
1
YSPWTNF
range2014626
1
4
24
annotation2014625
1
Stop
range2014625
1
25
29
annotation2014624
1
Start
range2014624
1
1
3
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986787
1
-10
range1986787
1
43
48
annotation1986785
1
-35
range1986785
1
20
25
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K237002_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgtattctccgtggaccaacttttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K237000_sequence
1
atgtattctccgtggaccaacttttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z