BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2000
1
-35
range2000
1
20
25
annotation1999
1
lac O1
range1999
1
3
19
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation2001
1
lac O1
range2001
1
26
42
annotation2002
1
-10
range2002
1
43
48
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K237003
1
sRIP
RIP +export signal (OmpA). Quorum sensing inhibitor of Staphylococcus spp. (Including MRSA)
2009-08-02T11:00:00Z
2015-05-08T01:11:36Z
The RIP peptide is produced by coagulase negative staphylococcus (suggested to be S. warnerii or S. xylosus) (16,19)
RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species. RIP effectively works as an antibiotic in its inhibition of bacterial growth.
RIP has the sequence YSPXTNF and has been tested extensively in a synthetic (as in organic) form for ability to inhibit bacterial infections in mice.
References will come later.
This is an export-tagged RIP brick. We used an OmpA tag, which (at least in theory) targets the peptide to the periplasmic space from where, there has been described numerous cases of leakage into the medium. Under the transport into the periplasmic space, the OmpA-leader should be cut off, leaving the RIP intact, and ready to inhibit some staph's.
We can at this point in our project not verify that RIP will be exported by our Coli's.
We have come to affectionately call this brick sRIP (short for signal-RIP, in contrast to just RIP).
false
false
_333_
0
4087
9
It's complicated
true
This sequence is reverse-engineered from the amino-acid sequence by using the commonly used codons of E. Coli.
It is designed to provide a big output of peptide. We just hope that this output won't kill the cell.
false
Marc T. K. Nielsen
annotation2014670
1
Stop
range2014670
1
85
89
annotation2014672
1
RIP
range2014672
1
64
84
annotation2014671
1
OmpA Leader
range2014671
1
4
63
annotation2014669
1
Start
range2014669
1
1
3
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K237006
1
BBa_K237006
IPTG inducible sRIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.
2009-08-02T11:00:00Z
2015-05-08T01:11:36Z
The RIP peptide is produced by coagulase negative staphylococcus (suggested to be S. warnerii or S. xylosus).
RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species. RIP effectively works as an antibiotic in its inhibition of bacterial growth.
RIP has the sequence YSPXTNF and has been shown through extensive research to inhibit bacterial infections in mice in a synthetic (as in organic synthesis) form.
The RIP produced by this generator is an export-tagged RIP brick. We used an OmpA tag, which (at least in theory) targets the peptide to the periplasmic space from where, there has been described numerous cases of leakage into the medium. Under the transport into the periplasmic space, the OmpA-leader should be cut off, leaving the RIP intact, and ready to inhibit some staph's.
References will come later.
false
false
_333_
0
4087
9
Not in stock
false
There are 4 versions of RIP producers from us.
1 - Constitutively produced RIP without an export sequence
2 - Inducibly produced RIP without an export sequence
3 - Constitutively produced RIP with an export sequence (OmpA)
4 - Inducibly produced RIP with an export sequence (OmpA)
This is number 4
false
Marc T. K. Nielsen
component2014691
1
BBa_R0011
component2014702
1
BBa_K237003
component2014703
1
BBa_B0010
component2014697
1
BBa_B0034
component2014705
1
BBa_B0012
annotation2014691
1
BBa_R0011
range2014691
1
1
54
annotation2014702
1
BBa_K237003
range2014702
1
82
171
annotation2014705
1
BBa_B0012
range2014705
1
268
308
annotation2014703
1
BBa_B0010
range2014703
1
180
259
annotation2014697
1
BBa_B0034
range2014697
1
64
75
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K237006_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcctattctccgtggaccaacttttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K237003_sequence
1
atgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcctattctccgtggaccaacttttaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z