BBa_K238001
1
BBa_K238001
VirB promoter and anti sense key
2009-07-14T11:00:00Z
2015-05-08T01:11:36Z
parts are both found in the registry
OmpF promoter regulated bij a vanilla sensing receptor and an anti sense key
false
false
_332_
0
4771
9
Not in stock
true
we used this specific key because of its succes. no RBS was needed.
false
Annelien Verfaillie
component2220490
1
BBa_K238011
component2220494
1
BBa_K238005
component2220501
1
BBa_B0015
annotation2220494
1
BBa_K238005
range2220494
1
245
580
annotation2220490
1
BBa_K238011
range2220490
1
1
236
annotation2220501
1
BBa_B0015
range2220501
1
589
717
BBa_K238005
1
antisense
Anti sense key
2009-07-15T11:00:00Z
2015-05-08T01:11:36Z
PCR on part J23066
the key part J23066 is inverted so that the anti sense version is transcribed.
false
false
_332_
0
4771
9
It's complicated
true
terminators need to be removed
false
Annelien Verfaillie
annotation2011532
1
J23009
range2011532
1
1
97
annotation2011531
1
J23008
range2011531
1
106
199
annotation2027991
1
stop codons
range2027991
1
200
336
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K238011
1
VirB
VirB promoter region
2009-07-27T11:00:00Z
2015-05-08T01:11:36Z
Agrobacterium tumefaciens pTiBo542 and pTiA6NC/A1011
This is the region where the "vir box" can be found. VirG will bind here and stimulate the transcription of the genes that are downstream of this promoter.
false
false
_332_
0
4771
9
It's complicated
true
none
false
Annelien Verfaillie
annotation2013553
1
vir box
range2013553
1
133
144
annotation2013530
1
vir box
range2013530
1
113
124
annotation2013531
1
rbs
range2013531
1
231
236
annotation2013568
1
pt -10 box
range2013568
1
167
173
annotation2013829
1
pt -35 box
range2013829
1
145
150
annotation2013828
1
transcription start
range2013828
1
180
181
annotation2013567
1
alt transcription start
range2013567
1
182
183
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K238005_sequence
1
cgggcgggcgggcgggcgggaagctttttttttttaactagaatcacctcttgcttttgggttttttttttatggatcccgcccgcccgcccgcccgctctagtaaagccggattaataatctggctttttagagatatttctagtaagtaagttaattttcattaaccaccaactagaatcacctcttgcttttgggttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K238001_sequence
1
ttcgttctgaggccgacctgaccaactgcggtctgtcaatccggttgctttgtcagggatgcgcggttctcggtccatgttttgttccaagacgccgagcgaaggttttcgcttcaattgaaatcataaagaagcaattgaaaattttcgagtaaccgaccctcccgataatcgtcaacataaaacaacgcacttcttccaacgggagaggcggtgttagttgcgagctaaggagatactagagcgggcgggcgggcgggcgggaagctttttttttttaactagaatcacctcttgcttttgggttttttttttatggatcccgcccgcccgcccgcccgctctagtaaagccggattaataatctggctttttagagatatttctagtaagtaagttaattttcattaaccaccaactagaatcacctcttgcttttgggttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K238011_sequence
1
ttcgttctgaggccgacctgaccaactgcggtctgtcaatccggttgctttgtcagggatgcgcggttctcggtccatgttttgttccaagacgccgagcgaaggttttcgcttcaattgaaatcataaagaagcaattgaaaattttcgagtaaccgaccctcccgataatcgtcaacataaaacaacgcacttcttccaacgggagaggcggtgttagttgcgagctaaggaga
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z