LOCUS BBa_K238003 4567 bp DNA linear 15-Nov-2024 DEFINITION Vanillin receptor ACCESSION BBa_K238003 VERSION BBa_K238003.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 RBS 44..56 /label=RBS-3\Weak RBS 50..53 /label=RBS misc_feature 44..56 /label=BBa_B0032 RBS 44..56 /label=BBa_B0032 CDS 63..2549 /label=VirA misc_feature 2550..2555 /label=stop misc_feature 246..251 /label=mutated PstI misc_feature 780..785 /label=mutated PstI CDS 63..2555 /label=BBa_K238008 RBS 2564..2576 /label=RBS-3\Weak RBS 2570..2573 /label=RBS misc_feature 2564..2576 /label=BBa_B0032 RBS 2564..2576 /label=BBa_B0032 CDS 2583..3380 /label=VirG misc_feature 3381..3386 /label=stop CDS 2583..3389 /label=BBa_K238009 RBS 3398..3410 /label=RBS-3\Weak RBS 3404..3407 /label=RBS misc_feature 3398..3410 /label=BBa_B0032 RBS 3398..3410 /label=BBa_B0032 CDS 3417..4421 /label=RpoA misc_feature 3618..3623 /label=mutated EcoRI misc_feature 3843..3848 /label=mutated EcoRI misc_feature 4422..4427 /label=stop CDS 3417..4430 /label=BBa_K238010 stem_loop 4450..4493 /label=stem_loop misc_feature 4439..4518 /label=BBa_B0010 terminator 4439..4518 /label=BBa_B0010 stem_loop 4534..4553 /label=T7 TE misc_feature 4560..4560 /label=stop polyA_site 4554..4567 /label=polya misc_feature 4527..4567 /label=BBa_B0012 terminator 4527..4567 /label=BBa_B0012 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagtcacaca ggaaagtact 61 agatgaacgg aagatattca ccgacgcggc aggattttaa gacaggcgcg aagccttggt 121 ctatattggc ccttatcgtt gctgcaatga ttttcgcgtt catggcggtt gcgtcctggc 181 aggacaatgc gactacccag gcaatcctca gccaactacg atcgattaac gccgacagcg 241 cctcactgca acgcgatgta ctccgcgctc acacgggcac cgtggcgaac taccgcccca 301 ttatctccag gctgggagct ctgcggaaga atctggaaga tttgaagcaa ttatttagac 361 aatctcatat tgtaagtgag agcaatgctg ctcaactgct acgccagcta gaagtgtctc 421 taaattcggc tgacgcggcg gtcgccgcct ttggtgcgca aaatgtacgc ctgcaagatt 481 cgctggccag tttcactcgt gctttgagca gtcttccagg aaaagcctca accgatcaga 541 ctttagaaaa accaacagaa ttggctagca tgatgctcca atttcttcgg caaccaagcc 601 cggctatttc attcgagatc agccttgaac tagagaggct ccaaaaacaa cgcggtcttg 661 atgaagctcc cgtgcgcata cttgcacgtg aaggtcccat tatcttatcg cttttgccac 721 aggtgaaaga tctggtgaac atgattcaga cgtctgacac cgcagaaatt gcggagatgc 781 tgcaacgcga gtgtttggag gtctatagct tgaaaaatgt agaggagcgg agcgcacgta 841 tctttcttgg gtccgcttca gtgggtcttt gcctctacat catcacctta gtctataggc 901 tacgcaaaaa aaccgattgg ttagcgcggc gtttagatta cgaagagcta atcaaagaga 961 tcggagtatg ttttgaaggt gaggcggcca ccacgtcgtc cgcgcaagct gcacttcgta 1021 ttattcagcg cttctttgat gccgatacgt gcgcgttagc tctagtggac catgaccgta 1081 gatgggctgt cgaaacattc ggtgcgaaac acccaaaacc tgtgtgggac gacagcgtgc 1141 tacgcgaaat agtctctcgt accaaagcgg acgaacgggc gacggtattc cgcatcatat 1201 cgtcgaaaaa aatcgtacat ttgcctctcg aaattccagg tctctcgata ctactggctc 1261 acaaatccac agataaacta attgcggttt gttcactggg ttaccaaagc tatcgccctc 1321 gaccttgcca aggcgaaatt cagcttcttg aactcgccac cgcctgcctc tgtcactata 1381 tcgatgttcg gcgtaagcag accgaatgcg acgttttggc cagacgattg gagcatgcgc 1441 aacgccttga ggcagttggt acacttgccg gcggaatagc acatgaattt aataacattt 1501 tgggctcaat cctcgggcac gcagaattag cacaaaactc ggtgtctcga acatctgtca 1561 cccgaagata tattgactat atcatttcgt caggcgacag agccatgctc attatcgatc 1621 agatcttgac gctgagccga aaacaggagc gcatgatcaa gccatttagt gtctcagagc 1681 ttgtgaccga aatcgctccc ttgctacgta tggctcttcc gccaaacatc gagcttagtt 1741 tcagatttga tcaaatgcag agcgtgatcg aaggaagccc gcttgaactt caacaggtac 1801 taattaacat ctgcaagaat gcttcccaag ccatgactgc aaatggtcaa atcgacatca 1861 tcatcagcca agctttttta ccagttaaga aaattctggc gcatggtgtt atgccacctg 1921 gcgactatgt tctcctatct attagcgaca atggtggagg cattcccgag gctgtgttac 1981 cccacatttt tgaacccttc tttacgacac gagctcgcaa cggtggaacg ggtctcggcc 2041 ttgcttctgt gcatggtcat atcagcgcgt ttgcgggtta catcgacgtt agttcaactg 2101 ttgggcatgg gacgcgcttt gacatttatc tccctccgtc ttctaaggaa cccgtaaatc 2161 cagacagttt tttcggccgc aataaggcac cgcgtggaaa cggggagatt gtggcacttg 2221 ttgagcccga tgacctcctg cgggaggcgt atgaagacaa gatcgccgct ctaggatatg 2281 agccggtcgg ttttcgtacc tttaatgaaa ttcgcgattg gatttcaaaa ggcaatgaag 2341 ccgatctggt catggtcgac caagcgtctc ttcctgaaga tcaaagtcct aattccgtgg 2401 atttagtgct caagaccgcc tccatcatca ttggcggaaa tgatctcaaa atgacccttt 2461 caagggagga tgtgaccagg gacctttatc ttccgaagcc gatatcgtcc agaactatgg 2521 cgcatgcaat cctaaccaaa atcaagacgt agtaatacta gagtcacaca ggaaagtact 2581 agatgattgt acatccttca cgtgaaaatt tctcaagcgc tgtgaacaag ggttcagatt 2641 ttagattgaa aggtgagccg ttgaaacacg ttcttcttgt cgatgacgac gtcgctatgc 2701 ggcatcttat tattgaatac cttacgatcc acgccttcaa agtgaccgcg gtagccgaca 2761 gcacccagtt cacaagagta ctctcttccg cgacggtcga tgtcgtggtt gttgatctaa 2821 atttaggtcg tgaagatggg ctcgagatcg ttcgtaatct ggcggcaaag tctgatattc 2881 caatcataat tatcagtggc gaccgccttg aggagacgga taaagttgtt gcactcgagc 2941 taggagcaag tgattttatc gctaagccgt tcagtatcag agagtttcta gcacgcattc 3001 gggttgcctt gcgcgtgcgc cccaacgttg tccgctccaa agaccgacgg tctttttgtt 3061 ttactgactg gacacttaat ctcaggcaac gtcgcttgat gtccgaagct ggcggtgagg 3121 tgaaacttac ggcaggtgag ttcaatcttc tcctcgcgtt tttagagaaa ccccgcgacg 3181 ttctatcgcg cgagcaactt ctcattgcca gtcgagtacg cgacgaggag gtttatgaca 3241 ggagtataga tgttctcatt ttgaggctgc gccgcaaact tgaggcggat ccgtcaagcc 3301 ctcaactgat aaaaacagca agaggtgccg gttatttctt tgacgcggac gtgcaggttt 3361 cgcacggggg gacgatggca gcctgataat actagagtca cacaggaaag tactagatga 3421 ttcagaagaa ctggcaggaa cttatcaagc cgaacaaggt cgagttcacc tcgtccagcc 3481 gcaccaaggc aactctggtt gccgagccgc tggagcgtgg tttcggtctt accctcggca 3541 acgcgctgcg ccgcgttctg ttgtcttctc tgcgtggtgc cgctgtaacg gccgtgcaga 3601 tcgacggtgt cctgcacgaa ttttcctcca tccccggcgt tcgggaagat gtgacggata 3661 tcgtgctcaa catcaaggaa atcgccatca agatggatgg tgacgattcc aagcgcatgg 3721 tcgtgcgcaa gcagggtccg ggttcggtaa ccgctggtga catccagacg gttggcgaca 3781 tcgagatcct gaaccccgac cacgtgatct gcacgctcga tgaaggcgct gaaatccgca 3841 tggaatttac cgtcaacaac ggcaagggtt acgtaccggc tgagcgcaac cgcgcggaag 3901 atgcccctat cggcctcatt ccggtggaca gcctctattc tccggtcaag aaagtgtcct 3961 acaaggtgga aaacacccgt gaaggtcagg ttctcgacta tgacaagctg atcatgacga 4021 tcgagaccaa cggttcggtt tccggcgaag acgccgttgc cttcgccgct cgcattcttc 4081 aggaccagct gggcgtcttc gtcaacttcg acgagccgca gaaggaagca gaagaagaat 4141 cggttactga actcgcgttc aacccggcgc ttctcaagaa ggtcgacgag ctcgaactgt 4201 cagttcgttc ggcaaactgc ctgaagaacg acaacatcgt ttatatcggc gacctgatcc 4261 agaagaccga agccgaaatg ctccgcacgc cgaactttgg tcgcaagtcg ctgaacgaaa 4321 tcaaggaagt tctcgcttcc atgggtctgc acctcggcat ggaagtgccg gcatggccgc 4381 ctgagaacat cgaagatctc gcaaagcgtt acgaagacca atactaataa tactagagcc 4441 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4501 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 4561 gtttata //