##gff-version 3
##sequence-region BBa_K238003 1 4567
BBa_K238003	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K238003	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K238003	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K238003	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K238003	.	terminator	4439	4518	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K238003	.	stem_loop	4450	4493	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K238003	.	engineered_region	4439	4518	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K238003	.	ribosome_entry_site	2564	2576	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K238003	.	ribosome_entry_site	2570	2573	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K238003	.	ribosome_entry_site	2564	2576	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K238003	.	engineered_region	2564	2576	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K238003	.	ribosome_entry_site	3398	3410	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K238003	.	ribosome_entry_site	3404	3407	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K238003	.	ribosome_entry_site	3398	3410	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K238003	.	engineered_region	3398	3410	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K238003	.	terminator	4527	4567	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K238003	.	stem_loop	4534	4553	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K238003	.	stop_codon	4560	4560	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K238003	.	polyA_site	4554	4567	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K238003	.	engineered_region	4527	4567	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K238003	.	CDS	63	2555	.	+	0	ID=BBa_K238008;Name=BBa_K238008
BBa_K238003	.	stop_codon	2550	2555	.	+	0	ID=annotation2013482;Name=stop;Parent=BBa_K238008
BBa_K238003	.	CDS	63	2549	.	+	0	ID=annotation2013377;Name=VirA;Parent=BBa_K238008
BBa_K238003	.	sequence_feature	246	251	.	+	0	ID=annotation2013695;Name=mutated PstI;Parent=BBa_K238008
BBa_K238003	.	sequence_feature	780	785	.	+	0	ID=annotation2013696;Name=mutated PstI;Parent=BBa_K238008
BBa_K238003	.	CDS	3417	4430	.	+	0	ID=BBa_K238010;Name=BBa_K238010
BBa_K238003	.	sequence_feature	3618	3623	.	+	0	ID=annotation2013480;Name=mutated EcoRI;Parent=BBa_K238010
BBa_K238003	.	CDS	3417	4421	.	+	0	ID=annotation2013379;Name=RpoA;Parent=BBa_K238010
BBa_K238003	.	sequence_feature	3843	3848	.	+	0	ID=annotation2013481;Name=mutated EcoRI;Parent=BBa_K238010
BBa_K238003	.	stop_codon	4422	4427	.	+	0	ID=annotation2013484;Name=stop;Parent=BBa_K238010
BBa_K238003	.	CDS	2583	3389	.	+	0	ID=BBa_K238009;Name=BBa_K238009
BBa_K238003	.	CDS	2583	3380	.	+	0	ID=annotation2013378;Name=VirG;Parent=BBa_K238009
BBa_K238003	.	stop_codon	3381	3386	.	+	0	ID=annotation2013483;Name=stop;Parent=BBa_K238009
BBa_K238003	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110
>BBa_K238003
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgaacggaagatattc
accgacgcggcaggattttaagacaggcgcgaagccttggtctatattggcccttatcgttgctgcaatgattttcgcg
ttcatggcggttgcgtcctggcaggacaatgcgactacccaggcaatcctcagccaactacgatcgattaacgccgaca
gcgcctcactgcaacgcgatgtactccgcgctcacacgggcaccgtggcgaactaccgccccattatctccaggctggg
agctctgcggaagaatctggaagatttgaagcaattatttagacaatctcatattgtaagtgagagcaatgctgctcaa
ctgctacgccagctagaagtgtctctaaattcggctgacgcggcggtcgccgcctttggtgcgcaaaatgtacgcctgc
aagattcgctggccagtttcactcgtgctttgagcagtcttccaggaaaagcctcaaccgatcagactttagaaaaacc
aacagaattggctagcatgatgctccaatttcttcggcaaccaagcccggctatttcattcgagatcagccttgaacta
gagaggctccaaaaacaacgcggtcttgatgaagctcccgtgcgcatacttgcacgtgaaggtcccattatcttatcgc
ttttgccacaggtgaaagatctggtgaacatgattcagacgtctgacaccgcagaaattgcggagatgctgcaacgcga
gtgtttggaggtctatagcttgaaaaatgtagaggagcggagcgcacgtatctttcttgggtccgcttcagtgggtctt
tgcctctacatcatcaccttagtctataggctacgcaaaaaaaccgattggttagcgcggcgtttagattacgaagagc
taatcaaagagatcggagtatgttttgaaggtgaggcggccaccacgtcgtccgcgcaagctgcacttcgtattattca
gcgcttctttgatgccgatacgtgcgcgttagctctagtggaccatgaccgtagatgggctgtcgaaacattcggtgcg
aaacacccaaaacctgtgtgggacgacagcgtgctacgcgaaatagtctctcgtaccaaagcggacgaacgggcgacgg
tattccgcatcatatcgtcgaaaaaaatcgtacatttgcctctcgaaattccaggtctctcgatactactggctcacaa
atccacagataaactaattgcggtttgttcactgggttaccaaagctatcgccctcgaccttgccaaggcgaaattcag
cttcttgaactcgccaccgcctgcctctgtcactatatcgatgttcggcgtaagcagaccgaatgcgacgttttggcca
gacgattggagcatgcgcaacgccttgaggcagttggtacacttgccggcggaatagcacatgaatttaataacatttt
gggctcaatcctcgggcacgcagaattagcacaaaactcggtgtctcgaacatctgtcacccgaagatatattgactat
atcatttcgtcaggcgacagagccatgctcattatcgatcagatcttgacgctgagccgaaaacaggagcgcatgatca
agccatttagtgtctcagagcttgtgaccgaaatcgctcccttgctacgtatggctcttccgccaaacatcgagcttag
tttcagatttgatcaaatgcagagcgtgatcgaaggaagcccgcttgaacttcaacaggtactaattaacatctgcaag
aatgcttcccaagccatgactgcaaatggtcaaatcgacatcatcatcagccaagcttttttaccagttaagaaaattc
tggcgcatggtgttatgccacctggcgactatgttctcctatctattagcgacaatggtggaggcattcccgaggctgt
gttaccccacatttttgaacccttctttacgacacgagctcgcaacggtggaacgggtctcggccttgcttctgtgcat
ggtcatatcagcgcgtttgcgggttacatcgacgttagttcaactgttgggcatgggacgcgctttgacatttatctcc
ctccgtcttctaaggaacccgtaaatccagacagttttttcggccgcaataaggcaccgcgtggaaacggggagattgt
ggcacttgttgagcccgatgacctcctgcgggaggcgtatgaagacaagatcgccgctctaggatatgagccggtcggt
tttcgtacctttaatgaaattcgcgattggatttcaaaaggcaatgaagccgatctggtcatggtcgaccaagcgtctc
ttcctgaagatcaaagtcctaattccgtggatttagtgctcaagaccgcctccatcatcattggcggaaatgatctcaa
aatgaccctttcaagggaggatgtgaccagggacctttatcttccgaagccgatatcgtccagaactatggcgcatgca
atcctaaccaaaatcaagacgtagtaatactagagtcacacaggaaagtactagatgattgtacatccttcacgtgaaa
atttctcaagcgctgtgaacaagggttcagattttagattgaaaggtgagccgttgaaacacgttcttcttgtcgatga
cgacgtcgctatgcggcatcttattattgaataccttacgatccacgccttcaaagtgaccgcggtagccgacagcacc
cagttcacaagagtactctcttccgcgacggtcgatgtcgtggttgttgatctaaatttaggtcgtgaagatgggctcg
agatcgttcgtaatctggcggcaaagtctgatattccaatcataattatcagtggcgaccgccttgaggagacggataa
agttgttgcactcgagctaggagcaagtgattttatcgctaagccgttcagtatcagagagtttctagcacgcattcgg
gttgccttgcgcgtgcgccccaacgttgtccgctccaaagaccgacggtctttttgttttactgactggacacttaatc
tcaggcaacgtcgcttgatgtccgaagctggcggtgaggtgaaacttacggcaggtgagttcaatcttctcctcgcgtt
tttagagaaaccccgcgacgttctatcgcgcgagcaacttctcattgccagtcgagtacgcgacgaggaggtttatgac
aggagtatagatgttctcattttgaggctgcgccgcaaacttgaggcggatccgtcaagccctcaactgataaaaacag
caagaggtgccggttatttctttgacgcggacgtgcaggtttcgcacggggggacgatggcagcctgataatactagag
tcacacaggaaagtactagatgattcagaagaactggcaggaacttatcaagccgaacaaggtcgagttcacctcgtcc
agccgcaccaaggcaactctggttgccgagccgctggagcgtggtttcggtcttaccctcggcaacgcgctgcgccgcg
ttctgttgtcttctctgcgtggtgccgctgtaacggccgtgcagatcgacggtgtcctgcacgaattttcctccatccc
cggcgttcgggaagatgtgacggatatcgtgctcaacatcaaggaaatcgccatcaagatggatggtgacgattccaag
cgcatggtcgtgcgcaagcagggtccgggttcggtaaccgctggtgacatccagacggttggcgacatcgagatcctga
accccgaccacgtgatctgcacgctcgatgaaggcgctgaaatccgcatggaatttaccgtcaacaacggcaagggtta
cgtaccggctgagcgcaaccgcgcggaagatgcccctatcggcctcattccggtggacagcctctattctccggtcaag
aaagtgtcctacaaggtggaaaacacccgtgaaggtcaggttctcgactatgacaagctgatcatgacgatcgagacca
acggttcggtttccggcgaagacgccgttgccttcgccgctcgcattcttcaggaccagctgggcgtcttcgtcaactt
cgacgagccgcagaaggaagcagaagaagaatcggttactgaactcgcgttcaacccggcgcttctcaagaaggtcgac
gagctcgaactgtcagttcgttcggcaaactgcctgaagaacgacaacatcgtttatatcggcgacctgatccagaaga
ccgaagccgaaatgctccgcacgccgaactttggtcgcaagtcgctgaacgaaatcaaggaagttctcgcttccatggg
tctgcacctcggcatggaagtgccggcatggccgcctgagaacatcgaagatctcgcaaagcgttacgaagaccaatac
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata