BBa_K238010 1 rpoA RpoA 2009-07-27T11:00:00Z 2015-05-08T01:11:36Z Agrobacterium tumefaciens pTiBo542 and pTiA6NC/A1011 this is an alfa subunit polymerase. it is necessary in E.Coli so that the Vir box can be transcribed properly. false false _332_ 0 4771 9 Not in stock true none false Annelien Verfaillie annotation2013481 1 mutated EcoRI range2013481 1 427 432 annotation2013480 1 mutated EcoRI range2013480 1 202 207 annotation2013379 1 RpoA range2013379 1 1 1005 annotation2013484 1 stop range2013484 1 1006 1011 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K238008 1 VirA VirA 2009-07-27T11:00:00Z 2015-05-08T01:11:36Z Agrobacterium tumefaciens pTiBo542 and pTIA6NC/A1011 This is Vir A, a sensor originally found in Agrobacterium tumefaciens, plasmid Ti. It is able to sense Phenols and as a reaction regulate gene transcription. false false _332_ 0 4771 9 It's complicated true no false Annelien Verfaillie annotation2013695 1 mutated PstI range2013695 1 184 189 annotation2013377 1 VirA range2013377 1 1 2487 annotation2013696 1 mutated PstI range2013696 1 718 723 annotation2013482 1 stop range2013482 1 2488 2493 BBa_K238003 1 BBa_K238003 Vanillin receptor 2009-07-14T11:00:00Z 2015-05-08T01:11:36Z all parts can be found in the registry this receptor is composed of a PBP that binds the vanilla. this complex then binds to the Trg-EnvZ fusion in the membrane. this fusion works intracellular through the ompR transcriptional factor. false false _332_ 0 4771 9 Not in stock true the promoter should not be to weak. false Annelien Verfaillie component2030090 1 BBa_B0032 component2030105 1 BBa_B0012 component2030102 1 BBa_K238010 component2030103 1 BBa_B0010 component2030088 1 BBa_K238008 component2030080 1 BBa_J23110 component2030082 1 BBa_B0032 component2030096 1 BBa_B0032 component2030094 1 BBa_K238009 annotation2030094 1 BBa_K238009 range2030094 1 2583 3389 annotation2030096 1 BBa_B0032 range2030096 1 3398 3410 annotation2030103 1 BBa_B0010 range2030103 1 4439 4518 annotation2030082 1 BBa_B0032 range2030082 1 44 56 annotation2030102 1 BBa_K238010 range2030102 1 3417 4430 annotation2030090 1 BBa_B0032 range2030090 1 2564 2576 annotation2030088 1 BBa_K238008 range2030088 1 63 2555 annotation2030105 1 BBa_B0012 range2030105 1 4527 4567 annotation2030080 1 BBa_J23110 range2030080 1 1 35 BBa_K238009 1 VirG VirG 2009-07-27T11:00:00Z 2015-05-08T01:11:36Z Agrobacterium tumefaciens plasmid pTiBo542 and pTiA6NC/A1011 VirG is a an activator. It is activated bij VirA when this senses phenols. after activation, VirG will bind to the Vir box and activate gene transcription false false _332_ 0 4771 9 It's complicated true none false Annelien Verfaillie annotation2013378 1 VirG range2013378 1 1 798 annotation2013483 1 stop range2013483 1 799 804 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K238009_sequence 1 atgattgtacatccttcacgtgaaaatttctcaagcgctgtgaacaagggttcagattttagattgaaaggtgagccgttgaaacacgttcttcttgtcgatgacgacgtcgctatgcggcatcttattattgaataccttacgatccacgccttcaaagtgaccgcggtagccgacagcacccagttcacaagagtactctcttccgcgacggtcgatgtcgtggttgttgatctaaatttaggtcgtgaagatgggctcgagatcgttcgtaatctggcggcaaagtctgatattccaatcataattatcagtggcgaccgccttgaggagacggataaagttgttgcactcgagctaggagcaagtgattttatcgctaagccgttcagtatcagagagtttctagcacgcattcgggttgccttgcgcgtgcgccccaacgttgtccgctccaaagaccgacggtctttttgttttactgactggacacttaatctcaggcaacgtcgcttgatgtccgaagctggcggtgaggtgaaacttacggcaggtgagttcaatcttctcctcgcgtttttagagaaaccccgcgacgttctatcgcgcgagcaacttctcattgccagtcgagtacgcgacgaggaggtttatgacaggagtatagatgttctcattttgaggctgcgccgcaaacttgaggcggatccgtcaagccctcaactgataaaaacagcaagaggtgccggttatttctttgacgcggacgtgcaggtttcgcacggggggacgatggcagcctgataa BBa_K238003_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgaacggaagatattcaccgacgcggcaggattttaagacaggcgcgaagccttggtctatattggcccttatcgttgctgcaatgattttcgcgttcatggcggttgcgtcctggcaggacaatgcgactacccaggcaatcctcagccaactacgatcgattaacgccgacagcgcctcactgcaacgcgatgtactccgcgctcacacgggcaccgtggcgaactaccgccccattatctccaggctgggagctctgcggaagaatctggaagatttgaagcaattatttagacaatctcatattgtaagtgagagcaatgctgctcaactgctacgccagctagaagtgtctctaaattcggctgacgcggcggtcgccgcctttggtgcgcaaaatgtacgcctgcaagattcgctggccagtttcactcgtgctttgagcagtcttccaggaaaagcctcaaccgatcagactttagaaaaaccaacagaattggctagcatgatgctccaatttcttcggcaaccaagcccggctatttcattcgagatcagccttgaactagagaggctccaaaaacaacgcggtcttgatgaagctcccgtgcgcatacttgcacgtgaaggtcccattatcttatcgcttttgccacaggtgaaagatctggtgaacatgattcagacgtctgacaccgcagaaattgcggagatgctgcaacgcgagtgtttggaggtctatagcttgaaaaatgtagaggagcggagcgcacgtatctttcttgggtccgcttcagtgggtctttgcctctacatcatcaccttagtctataggctacgcaaaaaaaccgattggttagcgcggcgtttagattacgaagagctaatcaaagagatcggagtatgttttgaaggtgaggcggccaccacgtcgtccgcgcaagctgcacttcgtattattcagcgcttctttgatgccgatacgtgcgcgttagctctagtggaccatgaccgtagatgggctgtcgaaacattcggtgcgaaacacccaaaacctgtgtgggacgacagcgtgctacgcgaaatagtctctcgtaccaaagcggacgaacgggcgacggtattccgcatcatatcgtcgaaaaaaatcgtacatttgcctctcgaaattccaggtctctcgatactactggctcacaaatccacagataaactaattgcggtttgttcactgggttaccaaagctatcgccctcgaccttgccaaggcgaaattcagcttcttgaactcgccaccgcctgcctctgtcactatatcgatgttcggcgtaagcagaccgaatgcgacgttttggccagacgattggagcatgcgcaacgccttgaggcagttggtacacttgccggcggaatagcacatgaatttaataacattttgggctcaatcctcgggcacgcagaattagcacaaaactcggtgtctcgaacatctgtcacccgaagatatattgactatatcatttcgtcaggcgacagagccatgctcattatcgatcagatcttgacgctgagccgaaaacaggagcgcatgatcaagccatttagtgtctcagagcttgtgaccgaaatcgctcccttgctacgtatggctcttccgccaaacatcgagcttagtttcagatttgatcaaatgcagagcgtgatcgaaggaagcccgcttgaacttcaacaggtactaattaacatctgcaagaatgcttcccaagccatgactgcaaatggtcaaatcgacatcatcatcagccaagcttttttaccagttaagaaaattctggcgcatggtgttatgccacctggcgactatgttctcctatctattagcgacaatggtggaggcattcccgaggctgtgttaccccacatttttgaacccttctttacgacacgagctcgcaacggtggaacgggtctcggccttgcttctgtgcatggtcatatcagcgcgtttgcgggttacatcgacgttagttcaactgttgggcatgggacgcgctttgacatttatctccctccgtcttctaaggaacccgtaaatccagacagttttttcggccgcaataaggcaccgcgtggaaacggggagattgtggcacttgttgagcccgatgacctcctgcgggaggcgtatgaagacaagatcgccgctctaggatatgagccggtcggttttcgtacctttaatgaaattcgcgattggatttcaaaaggcaatgaagccgatctggtcatggtcgaccaagcgtctcttcctgaagatcaaagtcctaattccgtggatttagtgctcaagaccgcctccatcatcattggcggaaatgatctcaaaatgaccctttcaagggaggatgtgaccagggacctttatcttccgaagccgatatcgtccagaactatggcgcatgcaatcctaaccaaaatcaagacgtagtaatactagagtcacacaggaaagtactagatgattgtacatccttcacgtgaaaatttctcaagcgctgtgaacaagggttcagattttagattgaaaggtgagccgttgaaacacgttcttcttgtcgatgacgacgtcgctatgcggcatcttattattgaataccttacgatccacgccttcaaagtgaccgcggtagccgacagcacccagttcacaagagtactctcttccgcgacggtcgatgtcgtggttgttgatctaaatttaggtcgtgaagatgggctcgagatcgttcgtaatctggcggcaaagtctgatattccaatcataattatcagtggcgaccgccttgaggagacggataaagttgttgcactcgagctaggagcaagtgattttatcgctaagccgttcagtatcagagagtttctagcacgcattcgggttgccttgcgcgtgcgccccaacgttgtccgctccaaagaccgacggtctttttgttttactgactggacacttaatctcaggcaacgtcgcttgatgtccgaagctggcggtgaggtgaaacttacggcaggtgagttcaatcttctcctcgcgtttttagagaaaccccgcgacgttctatcgcgcgagcaacttctcattgccagtcgagtacgcgacgaggaggtttatgacaggagtatagatgttctcattttgaggctgcgccgcaaacttgaggcggatccgtcaagccctcaactgataaaaacagcaagaggtgccggttatttctttgacgcggacgtgcaggtttcgcacggggggacgatggcagcctgataatactagagtcacacaggaaagtactagatgattcagaagaactggcaggaacttatcaagccgaacaaggtcgagttcacctcgtccagccgcaccaaggcaactctggttgccgagccgctggagcgtggtttcggtcttaccctcggcaacgcgctgcgccgcgttctgttgtcttctctgcgtggtgccgctgtaacggccgtgcagatcgacggtgtcctgcacgaattttcctccatccccggcgttcgggaagatgtgacggatatcgtgctcaacatcaaggaaatcgccatcaagatggatggtgacgattccaagcgcatggtcgtgcgcaagcagggtccgggttcggtaaccgctggtgacatccagacggttggcgacatcgagatcctgaaccccgaccacgtgatctgcacgctcgatgaaggcgctgaaatccgcatggaatttaccgtcaacaacggcaagggttacgtaccggctgagcgcaaccgcgcggaagatgcccctatcggcctcattccggtggacagcctctattctccggtcaagaaagtgtcctacaaggtggaaaacacccgtgaaggtcaggttctcgactatgacaagctgatcatgacgatcgagaccaacggttcggtttccggcgaagacgccgttgccttcgccgctcgcattcttcaggaccagctgggcgtcttcgtcaacttcgacgagccgcagaaggaagcagaagaagaatcggttactgaactcgcgttcaacccggcgcttctcaagaaggtcgacgagctcgaactgtcagttcgttcggcaaactgcctgaagaacgacaacatcgtttatatcggcgacctgatccagaagaccgaagccgaaatgctccgcacgccgaactttggtcgcaagtcgctgaacgaaatcaaggaagttctcgcttccatgggtctgcacctcggcatggaagtgccggcatggccgcctgagaacatcgaagatctcgcaaagcgttacgaagaccaatactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K238008_sequence 1 atgaacggaagatattcaccgacgcggcaggattttaagacaggcgcgaagccttggtctatattggcccttatcgttgctgcaatgattttcgcgttcatggcggttgcgtcctggcaggacaatgcgactacccaggcaatcctcagccaactacgatcgattaacgccgacagcgcctcactgcaacgcgatgtactccgcgctcacacgggcaccgtggcgaactaccgccccattatctccaggctgggagctctgcggaagaatctggaagatttgaagcaattatttagacaatctcatattgtaagtgagagcaatgctgctcaactgctacgccagctagaagtgtctctaaattcggctgacgcggcggtcgccgcctttggtgcgcaaaatgtacgcctgcaagattcgctggccagtttcactcgtgctttgagcagtcttccaggaaaagcctcaaccgatcagactttagaaaaaccaacagaattggctagcatgatgctccaatttcttcggcaaccaagcccggctatttcattcgagatcagccttgaactagagaggctccaaaaacaacgcggtcttgatgaagctcccgtgcgcatacttgcacgtgaaggtcccattatcttatcgcttttgccacaggtgaaagatctggtgaacatgattcagacgtctgacaccgcagaaattgcggagatgctgcaacgcgagtgtttggaggtctatagcttgaaaaatgtagaggagcggagcgcacgtatctttcttgggtccgcttcagtgggtctttgcctctacatcatcaccttagtctataggctacgcaaaaaaaccgattggttagcgcggcgtttagattacgaagagctaatcaaagagatcggagtatgttttgaaggtgaggcggccaccacgtcgtccgcgcaagctgcacttcgtattattcagcgcttctttgatgccgatacgtgcgcgttagctctagtggaccatgaccgtagatgggctgtcgaaacattcggtgcgaaacacccaaaacctgtgtgggacgacagcgtgctacgcgaaatagtctctcgtaccaaagcggacgaacgggcgacggtattccgcatcatatcgtcgaaaaaaatcgtacatttgcctctcgaaattccaggtctctcgatactactggctcacaaatccacagataaactaattgcggtttgttcactgggttaccaaagctatcgccctcgaccttgccaaggcgaaattcagcttcttgaactcgccaccgcctgcctctgtcactatatcgatgttcggcgtaagcagaccgaatgcgacgttttggccagacgattggagcatgcgcaacgccttgaggcagttggtacacttgccggcggaatagcacatgaatttaataacattttgggctcaatcctcgggcacgcagaattagcacaaaactcggtgtctcgaacatctgtcacccgaagatatattgactatatcatttcgtcaggcgacagagccatgctcattatcgatcagatcttgacgctgagccgaaaacaggagcgcatgatcaagccatttagtgtctcagagcttgtgaccgaaatcgctcccttgctacgtatggctcttccgccaaacatcgagcttagtttcagatttgatcaaatgcagagcgtgatcgaaggaagcccgcttgaacttcaacaggtactaattaacatctgcaagaatgcttcccaagccatgactgcaaatggtcaaatcgacatcatcatcagccaagcttttttaccagttaagaaaattctggcgcatggtgttatgccacctggcgactatgttctcctatctattagcgacaatggtggaggcattcccgaggctgtgttaccccacatttttgaacccttctttacgacacgagctcgcaacggtggaacgggtctcggccttgcttctgtgcatggtcatatcagcgcgtttgcgggttacatcgacgttagttcaactgttgggcatgggacgcgctttgacatttatctccctccgtcttctaaggaacccgtaaatccagacagttttttcggccgcaataaggcaccgcgtggaaacggggagattgtggcacttgttgagcccgatgacctcctgcgggaggcgtatgaagacaagatcgccgctctaggatatgagccggtcggttttcgtacctttaatgaaattcgcgattggatttcaaaaggcaatgaagccgatctggtcatggtcgaccaagcgtctcttcctgaagatcaaagtcctaattccgtggatttagtgctcaagaccgcctccatcatcattggcggaaatgatctcaaaatgaccctttcaagggaggatgtgaccagggacctttatcttccgaagccgatatcgtccagaactatggcgcatgcaatcctaaccaaaatcaagacgtagtaa BBa_K238010_sequence 1 atgattcagaagaactggcaggaacttatcaagccgaacaaggtcgagttcacctcgtccagccgcaccaaggcaactctggttgccgagccgctggagcgtggtttcggtcttaccctcggcaacgcgctgcgccgcgttctgttgtcttctctgcgtggtgccgctgtaacggccgtgcagatcgacggtgtcctgcacgaattttcctccatccccggcgttcgggaagatgtgacggatatcgtgctcaacatcaaggaaatcgccatcaagatggatggtgacgattccaagcgcatggtcgtgcgcaagcagggtccgggttcggtaaccgctggtgacatccagacggttggcgacatcgagatcctgaaccccgaccacgtgatctgcacgctcgatgaaggcgctgaaatccgcatggaatttaccgtcaacaacggcaagggttacgtaccggctgagcgcaaccgcgcggaagatgcccctatcggcctcattccggtggacagcctctattctccggtcaagaaagtgtcctacaaggtggaaaacacccgtgaaggtcaggttctcgactatgacaagctgatcatgacgatcgagaccaacggttcggtttccggcgaagacgccgttgccttcgccgctcgcattcttcaggaccagctgggcgtcttcgtcaacttcgacgagccgcagaaggaagcagaagaagaatcggttactgaactcgcgttcaacccggcgcttctcaagaaggtcgacgagctcgaactgtcagttcgttcggcaaactgcctgaagaacgacaacatcgtttatatcggcgacctgatccagaagaccgaagccgaaatgctccgcacgccgaactttggtcgcaagtcgctgaacgaaatcaaggaagttctcgcttccatgggtctgcacctcggcatggaagtgccggcatggccgcctgagaacatcgaagatctcgcaaagcgttacgaagaccaatactaataa BBa_B0032_sequence 1 tcacacaggaaag BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z