this receptor is composed of a PBP that binds the vanilla. this complex then binds to the Trg-EnvZ fusion in the membrane. this fusion works intracellular through the ompR transcriptional factor.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Transcriptional terminator consisting of a 64 bp stem-loop.
Agrobacterium tumefaciens pTiBo542 and pTIA6NC/A1011
This is Vir A, a sensor originally found in Agrobacterium tumefaciens, plasmid Ti. It is able to sense Phenols and as a reaction regulate gene transcription.
Agrobacterium tumefaciens plasmid pTiBo542 and pTiA6NC/A1011
VirG is a an activator. It is activated bij VirA when this senses phenols. after activation, VirG will bind to the Vir box and activate gene transcription
Agrobacterium tumefaciens pTiBo542 and pTiA6NC/A1011
this is an alfa subunit polymerase. it is necessary in E.Coli so that the Vir box can be transcribed properly.
Conversion of the iGEM parts registry to SBOL2.1