LOCUS BBa_K238004 7162 bp DNA linear 15-Nov-2024 DEFINITION Vanillin synthesis ACCESSION BBa_K238004 VERSION BBa_K238004.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 misc_feature 44..95 /label=BBa_J23078 CDS 102..1637 /label=BBa_I742142 RBS 1646..1661 /label=rbs CDS 1662..3203 /label=sam5 misc_feature 1646..3203 /label=BBa_K238007 misc_feature 3212..3263 /label=BBa_J23078 CDS 3270..4370 /label=COMT coding region CDS 3270..4370 /label=BBa_I742107 misc_feature 4379..4381 /label=SacI RBS 4382..4388 /label=rbs RBS 4379..4388 /label=BBa_J15001 CDS 4395..6167 /label=fcs coding region CDS 4395..6167 /label=BBa_I742114 misc_feature 6176..6178 /label=SacI RBS 6179..6185 /label=rbs RBS 6176..6185 /label=BBa_J15001 CDS 6192..7019 /label=ech coding region misc_feature 6192..6194 /label=start misc_feature 7020..7025 /label=stop CDS 6192..7025 /label=BBa_I742112 stem_loop 7045..7088 /label=stem_loop misc_feature 7034..7113 /label=BBa_B0010 terminator 7034..7113 /label=BBa_B0010 stem_loop 7129..7148 /label=T7 TE misc_feature 7155..7155 /label=stop polyA_site 7149..7162 /label=polya misc_feature 7122..7162 /label=BBa_B0012 terminator 7122..7162 /label=BBa_B0012 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagtgtagac cgaactagaa 61 tcacctcttg cttttgggta agacagaaga ggagatacta gatgacgcag gtcgtggaac 121 gtcaggctga tcggctcagc agcagggagt acctggcccg ggtcgtgcgc agcgccgggt 181 gggacgccgg tctcacctcg tgcaccgacg aggagatcgt ccggatgggc gcgagcgcgc 241 gcaccatcga ggagtacctg aagtccgaca agcccatcta cggcctgacg cagggcttcg 301 gtccgctggt gctgttcgac gccgactcgg agctggagca gggcggctcg ctgatctcgc 361 acctgggcac cggccagggc gcgccactgg ccccggaggt gtcgcggctg atcctctggc 421 tgcgcatcca gaacatgcgc aaggggtact cggcggtctc gccggtgttc tggcagaagc 481 tcgccgacct gtggaacaag gggttcaccc cggcgatccc ccggcacggc acggtcagcg 541 cgagcggcga cctgcaaccg ctggcgcacg ccgcgctcgc cttcaccggt gtcggcgagg 601 cgtggacccg ggacgccgac ggccggtggt ccaccgtgcc ggccgtggac gcgctcgccg 661 cgctgggggc ggagccgttc gactggccgg tgcgcgaggc gctggcgttc gtcaacggga 721 ccggcgcgag cctcgcggtg gctgtgctca accaccggtc cgccctgcgg ctggtccgcg 781 cctgcgccgt gctctccgcg cggctggcga ccctgctggg ggccaatccc gagcactacg 841 acgtggggca cggtgtcgcg cgcggccagg tcggtcagct gaccgcggcg gagtggatcc 901 ggcaggggct gccccggggc atggtgcgcg acggcagccg cccgctccag gagccgtaca 961 gcctgcggtg cgcgccgcag gtgctcggcg cggtgctcga ccagctcgac ggcgcgggcg 1021 acgtgctggc gcgggaggtc gacggctgcc aggacaaccc gatcacctac gagggcgagc 1081 tgctgcacgg cggcaacttc cacgccatgc cggtgggttt cgcctccgac cagatcgggt 1141 tggccatgca catggccgcc tacctggccg agcgccagct gggtctgctg gtcagcccgg 1201 tgaccaacgg cgacctgccg cccatgctca ccccgcgcgc cgggcgcggt gccgggctgg 1261 ccggggtgca gatcagcgcg acctcgttcg tctcgcggat ccggcagctg gtgttccccg 1321 cctcgctgac caccctgccg accaacggct ggaaccagga ccacgtgccg atggcgctca 1381 acggggcgaa ctcggtgttc gaggcgttgg agctcggctg gctgacggtc gggtcgctgg 1441 cggtgggcgt cgcgcagctc gcggccatga ccggccacgc cgcggagggc gtctgggcgg 1501 agctggccgg gatctgcccg ccgctggacg ccgaccgccc gctgggcgcc gaggtgcgcg 1561 ccgcgcgcga cctgctgtcc gcgcacgcgg accaactgct cgtcgacgag gcagacggga 1621 aggatttcgg ataataatac tagagacgga gaagcagcga aatgaccatc acgtcacctg 1681 cgccggcggg ccggctcaac aacgtccgcc cgatgacggg cgaggagtac ctggaatcac 1741 tgcgggacgg ccgagaggtc tacatctacg gcgagcgggt cgacgacgtc accacgcacc 1801 tggccttccg caacagcgtg cgctccatcg cgcggctcta cgacgtgctg cacgacccgg 1861 cgtccgaagg tgtgctgcgg gtgcccaccg acaccggcaa cggcgggttc acccacccgt 1921 tcttcaagac cgcccggtcg tcggaggacc tggtcgccgc gcgcgaggcc atcgtcggct 1981 ggcagcggct ggtgtacggg tggatgggcc gcaccccgga ctacaaggcg gcgttcttcg 2041 gcacgctcga cgccaacgcc gagttctacg ggccgttcga ggccaacgcc cgccgctggt 2101 accgcgacgc ccaggaacgg gtgctgtact tcaaccacgc gatcgtgcac ccgccggtcg 2161 accgggaccg gcccgccgac cggaccgccg acatctgcgt gcacgtggag gaggagaccg 2221 acagcgggtt gatcgtctcc ggcgccaagg tggtcgcgac cggctccgcg atgaccaacg 2281 cgaacctcat cgcgcactac gggcttccgg tgcgggacaa gaagttcggc ctggtgttca 2341 cggtcccgat gaactcgccc ggcctcaagc tcatctgccg cacctcctac gagctgatgg 2401 tcgcgacgca gggctcgccc ttcgactacc cgctgtcgag ccggctcgac gagaacgact 2461 cgatcatgat cttcgaccgg gtgctggtgc cctgggagaa cgtgttcatg tacgacgcgg 2521 gcgcggccaa ctccttcgcc accgggtcag gcttcctcga acgcttcacc ttccacggct 2581 gcacccgcct cgcggtcaag ctggacttca tcgccggctg cgtcatgaag gcggtggagg 2641 tcaccggcac cacgcacttc cggggcgtgc aggcgcaggt cggcgaagtg ctcaactggc 2701 gcgacgtgtt ctggggcctg tccgacgcga tggccaagtc gccgaactcg tgggtcggcg 2761 gctcggtgca gccgaacctc aactacgggc tcgcgtaccg caccttcatg ggcgtgggct 2821 acccgcgcat caaggagatc atccagcaga ccctcggcag cgggttgatc tacctgaact 2881 cctcggccgc cgactggaag aaccccgacg tccgcccgta cctcgaccgc tacctgcgcg 2941 gctcgcgggg catccaggcg atcgaccggg tcaagctgct gaagctgctg tgggacgcgg 3001 tcggcaccga gttcgccggc cggcacgagc tctacgagcg caactacggc ggcgaccacg 3061 agggcatccg ggtgcagacc ctgcaagcgt accaggcgaa cggccaagcc gccgcgctca 3121 agggtttcgc cgagcagtgc atgtccgagt acgacctcga cggctggacg aggcccgacc 3181 tgatcaaccc cggcacctaa taatactaga gtgtagaccg aactagaatc acctcttgct 3241 tttgggtaag acagaagagg agatactaga tgggttcaac aggtgaaact caaataacac 3301 caacccacat atcagatgaa gaagcaaacc tcttcgccat gcaactagca agtgcttcag 3361 ttcttcccat gattttgaaa tcagctcttg aacttgatct cttagaaatc attgctaaag 3421 ctggacctgg tgctcaaatt tcacctattg aaattgcttc tcagctacca acaactaacc 3481 ctgatgcacc agttatgttg gaccgaatgt tgcgtctctt ggcttgttac ataatcctca 3541 catgttcagt tcgtactcaa caagatggaa aggttcagag actttatggt ttggctactg 3601 ttgctaagta tttggttaag aatgaagatg gtgtatccat ttctgctctt aatctcatga 3661 atcaggataa agtgctcatg gaaagctggt accacctaaa agatgcagtc cttgatgggg 3721 gcattccatt caacaaggct tatggaatga cagcctttga ataccatgga acagatccaa 3781 ggtttaacaa ggttttcaac aaggggatgt ctgatcactc taccatcaca atgaagaaaa 3841 ttcttgagac ctacacaggt tttgaaggcc ttaaatctct tgttgatgta ggtggtggta 3901 ctggagctgt aattaacacg attgtctcaa aatatcccac tataaagggt ataaattttg 3961 atttacccca tgtcattgaa gatgctccat cttatccagg agttgagcat gttggtggag 4021 acatgtttgt cagtattcca aaggctgatg ctgtttttat gaagtggatt tgtcatgact 4081 ggagtgatga gcactgcttg aaatttttga agaactgcta tgaggcactg ccagacaatg 4141 gaaaagtgat tgtggcagaa tgcatacttc cagtggctcc agattcaagc ctggccacaa 4201 aaggtgtggt tcacattgat gtgatcatgt tggctcataa tcctggtggg aaagagagaa 4261 cacaaaaaga gtttgaggat cttgccaaag gtgctggatt ccaaggtttc aaagtccatt 4321 gtaatgcttt caacacatac atcatggagt ttcttaagaa ggtttaataa tactagagct 4381 caaggaggta ctagatgcgt agcctggaac cgctggccga actgccggca cgtctgctgg 4441 atcgtctggt gcattgggcg cgtgtgcgtc cggaacagac ctttattgcg gcgcgtcagg 4501 cgggtggtga ttggcgtcgt gtgagctatc gcgaaatgct ggatggcgtg cgtgcgattg 4561 cgcagggcct gctgtcttat ggcctgagcg cggataaacc gctggccctg ctgtctggca 4621 acgatattga acatctgcaa gtggcgctgg gcgcaatgta tgcgggcatt ccgtattgcc 4681 cggtgagccc ggcgtatagc ctgctgtctc aggattttgc gaaactgcgt catgtgtgcg 4741 atctgctgca accgggcctg gtttttgtgt ctgatgcgcc ggcatatcag cgtgcgattg 4801 atgcggttct gccgccggaa accccgctga ttagcgtgcg tggccaggtt ccgggccgta 4861 cgcagattag ctttgcgagc ctgctgcata ccagcgcggg caccgaagcg gatgcggcgt 4921 ttaacgcgac cggtccggat agcattgcga aatttctgtt taccagcggc agcaccaaac 4981 tgccgaaagc ggtgattacc acccagcgta tgctgtgcgc gaaccagcag atgctgctgc 5041 aaacctttcc ggtgtttggc gaagaaccgc cggtgctggt tgattggctg ccgtggaacc 5101 acacctttgg cggcagccat aacgtgggca ttgtgctgta taacggcggc accttttatc 5161 tggatgatgg caaaccgacc gcccagggct ttgcacagac gctgcgcaac ctgaaagaaa 5221 ttagcccgac cgcgtatctg accgtgccga aaggctggga agaactggtg aacgcgctgg 5281 aacaggatgc ggcgctgcgt gaacgttttt ttgcgcgcat gaaactgttt ttttttgcgg 5341 cggcaggcct gagccagagc gtgtgggatc gtctggatcg tgtggcggaa caacattgtg 5401 gcgaacgtat tcgcatgatg gcgggcctgg gcatgaccga agcggcaccg agctgcacct 5461 ttaccaccgg tccgctgtct atggcgggct atattggcct gccggcaccg ggttgtgaag 5521 tgcgtctggt gccggtggat ggcaaatttg aaggccgttt tcgtggcccg catatcatgc 5581 cgggttattg gcgtgcaccg gaacagaccg cggaagtgtt tgatgaacag ggctattatt 5641 gcagcggtga tgcgatcaaa ctggccgatc cgcatcagcc gcattggggc ctgatgtttg 5701 atggccgtat tgcggaagat tttaaactga gcagcggcgt gtttgtgtcc gtgggcccgc 5761 tgcgtaatcg tgcggttctg gaaggcagcc cgtatgtgca ggatctggtg attgcggcac 5821 cggatcgtga atgcctgggc gccctggttt ttccgcgtgc gtatgaatgc cgtcgtctga 5881 gcggcctgaa cgcggaagcg tctgatgcgg aagtgctgaa cagcgcaccg gttcgtcagt 5941 ggtttggcga ttggctgcaa cgtctgaacc gtgaagcgac cggcaatgcg agccgtctgg 6001 aatggatcgc ggtgctggat gaaccggcga gcattgatcg tggcgaaatt accgataaag 6061 gcagcattaa ccagcgcgcg gtgctgcaat ggcgtgcggc gaaagtggaa gcgctgtatc 6121 gtggcgaaga tccgaccatt ctgcgtgcgg gtccgaaacc gtaataatac tagagctcaa 6181 ggaggtacta gatgagcaaa tatgaaggcc gttggaccac cgtgaaagtg gaaattgaag 6241 aaggcattgc ctgggttatt ctgaaccgtc cggaaaaacg taacgccatg agcccgaccc 6301 tgaaccgtga aatgattgat gtgctggaaa ccctggaaca ggatccggcg gcgggtgttc 6361 tggttctgac cggtgcgggt gaagcgtgga ccgcgggcat ggatctgaaa gaatatttcc 6421 gtgaagtgga tgcgggtccg gaaattctgc aagaaaaaat tcgtcgcgaa gcgagccagt 6481 ggcagtggaa actgctgcgt atgtatgcga aaccgaccat tgccatggtg aacggctggt 6541 gctttggcgg cggttttagc ccgctggttg cgtgcgatct ggccatttgc gcggatgaag 6601 cgacctttgg cctgagcgag attaactggg gcattccgcc gggtaacctg gtgagcaaag 6661 ccatggcgga taccgtgggc catcgtcaga gcctgtatta tatcatgacc ggcaaaacct 6721 ttggtggcca gaaagcggcg gaaatgggcc tggtgaacga aagcgtgccg ctggcccagc 6781 tgcgtgaagt gaccattgaa ctggcccgta acctgctgga aaaaaatccg gtggtgctgc 6841 gtgcggcgaa acatggcttt aaacgttgcc gtgaactgac ctgggaacag aacgaagatt 6901 atctgtacgc gaaactggat cagagccgtc tgctggatac cgaaggcggc cgtgaacagg 6961 gcatgaaaca gtttctggat gataaaagca ttaaaccggg cctgcaagcg tataaacgtt 7021 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 7081 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 7141 ggtgggcctt tctgcgttta ta //