BBa_K238004
type: ComponentDefinition
persistent identity: BBa K238004
display id: BBa_K238004
version: 1
was generated by: iGEM to SBOL conversion
description: Vanillin synthesis
created: 2009-07-14T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: most parts were found in the registry.
owned by: james
owned by: myers
mutable description: this part is composed of several genes coding for 5 different enzymes which turn tyrosine into vanilla
discontinued: false
dominant: false
experience: experience/None
group u list: _332_
m user id: 0
owner id: 4771
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: parts for fcs (BBa_I742114) and ech (BBa_I742112) will probably not be used and made from another sequence.
star: false
creator: Annelien Verfaillie
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K238007
component: BBa_I742142
component: BBa_I742107
component: BBa_J23078
component: BBa_J23110
component: BBa_B0012
component: BBa_B0010
component: BBa_I742114
component: BBa_J15001
component: BBa_I742112
sequence annotation: BBa_J23110
sequence annotation: BBa_J23078
sequence annotation: BBa_I742142
sequence annotation: BBa_K238007
sequence annotation: BBa_I742107
sequence annotation: BBa_J15001
sequence annotation: BBa_I742114
sequence annotation: BBa_I742112
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K238004_sequence/1

BBa_K238007
type: Component
persistent identity: component2013301
display id: component2013301
version: 1
top level: BBa_K238004
definition: rbs + sam5
access: public

BBa_I742142
type: Component
persistent identity: component2013298
display id: component2013298
version: 1
top level: BBa_K238004
definition: sam8
access: public

BBa_I742107
type: Component
persistent identity: component2013304
display id: component2013304
version: 1
top level: BBa_K238004
definition: COMT
access: public

BBa_J23078
type: Component
persistent identity: component2013302
display id: component2013302
version: 1
top level: BBa_K238004
definition: BBa_J23078
access: public

BBa_J23078
type: Component
persistent identity: component2013297
display id: component2013297
version: 1
top level: BBa_K238004
definition: BBa_J23078
access: public

BBa_J23110
type: Component
persistent identity: component2013296
display id: component2013296
version: 1
top level: BBa_K238004
definition: BBa_J23110
access: public

BBa_B0012
type: Component
persistent identity: component2013319
display id: component2013319
version: 1
top level: BBa_K238004
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2013317
display id: component2013317
version: 1
top level: BBa_K238004
definition: BBa_B0010
access: public

BBa_I742114
type: Component
persistent identity: component2013309
display id: component2013309
version: 1
top level: BBa_K238004
definition: BBa_I742114
access: public

BBa_J15001
type: Component
persistent identity: component2013307
display id: component2013307
version: 1
top level: BBa_K238004
definition: BBa_J15001
access: public

BBa_I742112
type: Component
persistent identity: component2013316
display id: component2013316
version: 1
top level: BBa_K238004
definition: BBa_I742112
access: public

BBa_J15001
type: Component
persistent identity: component2013312
display id: component2013312
version: 1
top level: BBa_K238004
definition: BBa_J15001
access: public

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2013296
display id: annotation2013296
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013296/range2013296/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K238004/annotation2013296/range2013296/1
type: Range
persistent identity: range2013296
display id: range2013296
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_J23078
type: SequenceAnnotation
persistent identity: annotation2013297
display id: annotation2013297
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013297/range2013297/1
component: BBa_J23078

https://synbiohub.org/public/igem/BBa_K238004/annotation2013297/range2013297/1
type: Range
persistent identity: range2013297
display id: range2013297
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 44
end: 95
orientation: inline

BBa_I742142
type: SequenceAnnotation
persistent identity: annotation2013298
display id: annotation2013298
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013298/range2013298/1
component: BBa_I742142

https://synbiohub.org/public/igem/BBa_K238004/annotation2013298/range2013298/1
type: Range
persistent identity: range2013298
display id: range2013298
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 102
end: 1637
orientation: inline

BBa_K238007
type: SequenceAnnotation
persistent identity: annotation2013301
display id: annotation2013301
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013301/range2013301/1
component: BBa_K238007

https://synbiohub.org/public/igem/BBa_K238004/annotation2013301/range2013301/1
type: Range
persistent identity: range2013301
display id: range2013301
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 1646
end: 3203
orientation: inline

BBa_J23078
type: SequenceAnnotation
persistent identity: annotation2013302
display id: annotation2013302
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013302/range2013302/1
component: BBa_J23078

https://synbiohub.org/public/igem/BBa_K238004/annotation2013302/range2013302/1
type: Range
persistent identity: range2013302
display id: range2013302
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 3212
end: 3263
orientation: inline

BBa_I742107
type: SequenceAnnotation
persistent identity: annotation2013304
display id: annotation2013304
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013304/range2013304/1
component: BBa_I742107

https://synbiohub.org/public/igem/BBa_K238004/annotation2013304/range2013304/1
type: Range
persistent identity: range2013304
display id: range2013304
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 3270
end: 4370
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2013307
display id: annotation2013307
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013307/range2013307/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K238004/annotation2013307/range2013307/1
type: Range
persistent identity: range2013307
display id: range2013307
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 4379
end: 4388
orientation: inline

BBa_I742114
type: SequenceAnnotation
persistent identity: annotation2013309
display id: annotation2013309
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013309/range2013309/1
component: BBa_I742114

https://synbiohub.org/public/igem/BBa_K238004/annotation2013309/range2013309/1
type: Range
persistent identity: range2013309
display id: range2013309
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 4395
end: 6167
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2013312
display id: annotation2013312
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013312/range2013312/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K238004/annotation2013312/range2013312/1
type: Range
persistent identity: range2013312
display id: range2013312
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 6176
end: 6185
orientation: inline

BBa_I742112
type: SequenceAnnotation
persistent identity: annotation2013316
display id: annotation2013316
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013316/range2013316/1
component: BBa_I742112

https://synbiohub.org/public/igem/BBa_K238004/annotation2013316/range2013316/1
type: Range
persistent identity: range2013316
display id: range2013316
version: 1
top level: BBa_K238004
direction: direction/reverse
start: 6192
end: 7025
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2013317
display id: annotation2013317
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013317/range2013317/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K238004/annotation2013317/range2013317/1
type: Range
persistent identity: range2013317
display id: range2013317
version: 1
top level: BBa_K238004
direction: direction/forward
start: 7034
end: 7113
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2013319
display id: annotation2013319
version: 1
top level: BBa_K238004
location: https://synbiohub.org/public/igem/BBa_K238004/annotation2013319/range2013319/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K238004/annotation2013319/range2013319/1
type: Range
persistent identity: range2013319
display id: range2013319
version: 1
top level: BBa_K238004
direction: direction/forward
start: 7122
end: 7162
orientation: inline

BBa_J23078
type: ComponentDefinition
persistent identity: BBa J23078
display id: BBa_J23078
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3i]
created: 2006-09-12T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: PCR ca1028F/G00101 on pJ23006-J23032 . . . (162 bp, XbaI/PstI) Sub into pSB1A2-I13521 . . . . . . . . . . (XbaI/PstI) Product is pSB1A2-J23078
owned by: james
owned by: myers
mutable description: lock3d derivative with a 5' extension. This is the basic part, J23077 is the RFP reporter.
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23078_sequence/1

COMT
type: ComponentDefinition
persistent identity: BBa I742107
display id: BBa_I742107
version: 1
was generated by: iGEM to SBOL conversion
description: COMT
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Alfalfa cDNA Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: COMT coding sequence Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa), synthesised by Geneart
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Used cDNA as coding gene in Alfalfa genome contains introns.
star: true
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: COMT coding region
sequence: https://synbiohub.org/public/igem/BBa_I742107_sequence/1

COMT coding region
type: SequenceAnnotation
persistent identity: annotation1949817
display id: annotation1949817
version: 1
top level: COMT
location: https://synbiohub.org/public/igem/BBa_I742107/annotation1949817/range1949817/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I742107/annotation1949817/range1949817/1
type: Range
persistent identity: range1949817
display id: range1949817
version: 1
top level: COMT
direction: direction/forward
start: 1
end: 1101
orientation: inline

BBa_I742114
type: ComponentDefinition
persistent identity: BBa I742114
display id: BBa_I742114
version: 1
was generated by: iGEM to SBOL conversion
description: Feruloyl-CoA synthase (fcs)
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Pseudomonas fluorescens NCIMB 9046 Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
owned by: james
owned by: myers
mutable description: gene is from Pseudomonas fluorescens encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid) produces feruloyl CoA from ferrulic acid catalyses the penultimate of the vanillin biosynthesis pathway
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Gene was synthesised by geneart, as P. fluorescens has a high GC genome. Coding region was optimised for gene expression in E. coli
star: false
creator: sarah hollingshead
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: fcs coding region
sequence: https://synbiohub.org/public/igem/BBa_I742114_sequence/1

fcs coding region
type: SequenceAnnotation
persistent identity: annotation1949792
display id: annotation1949792
version: 1
top level: BBa_I742114
location: https://synbiohub.org/public/igem/BBa_I742114/annotation1949792/range1949792/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I742114/annotation1949792/range1949792/1
type: Range
persistent identity: range1949792
display id: range1949792
version: 1
top level: BBa_I742114
direction: direction/forward
start: 1
end: 1773
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

sam8
type: ComponentDefinition
persistent identity: BBa I742142
display id: BBa_I742142
version: 1
was generated by: iGEM to SBOL conversion
description: sam8 (tyrosine-ammonia lyase) coding sequence
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Saccharothrix espanaensis DSM 4429. Accession: ABC88666.
owned by: james
owned by: myers
mutable description: This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: No important considerations.
star: false
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I742142_sequence/1

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

rbs + sam5
type: ComponentDefinition
persistent identity: BBa K238007
display id: BBa_K238007
version: 1
was generated by: iGEM to SBOL conversion
description: Sam5 gene with RBS
created: 2009-07-26T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: lab of Prof. C. French of the University of Edinburgh
owned by: james
owned by: myers
mutable description: This is an improved version of the Sam5 gene (BBa_I742105). The restriction site for PstI that was initially found at location 1436 was mutated out. The mutagenesis was done by Mr. Rashed Al Naber under supervision of Prof. C. French of the University of Edinburgh, in March 2008, using BioBrick BBa_I742105 as a template (this BioBrick was prepared by the Edinburgh iGEM 2007 team but was not submitted to the Registry).
discontinued: false
dominant: false
experience: experience/None
group u list: _332_
m user id: 0
owner id: 4771
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: the PstI restriction site was mutated out.
star: false
creator: Annelien Verfaillie
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: rbs
sequence annotation: sam5
sequence: https://synbiohub.org/public/igem/BBa_K238007_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2013240
display id: annotation2013240
version: 1
top level: rbs + sam5
location: https://synbiohub.org/public/igem/BBa_K238007/annotation2013240/range2013240/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K238007/annotation2013240/range2013240/1
type: Range
persistent identity: range2013240
display id: range2013240
version: 1
top level: rbs + sam5
direction: direction/forward
start: 1
end: 16
orientation: inline

sam5
type: SequenceAnnotation
persistent identity: annotation2013241
display id: annotation2013241
version: 1
top level: rbs + sam5
location: https://synbiohub.org/public/igem/BBa_K238007/annotation2013241/range2013241/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K238007/annotation2013241/range2013241/1
type: Range
persistent identity: range2013241
display id: range2013241
version: 1
top level: rbs + sam5
direction: direction/forward
start: 17
end: 1558
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I742112
type: ComponentDefinition
persistent identity: BBa I742112
display id: BBa_I742112
version: 1
was generated by: iGEM to SBOL conversion
description: enoyl CoA hydratase (ech) gene
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Pseudomonas fluorescens NCIMB 9046 Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
owned by: james
owned by: myers
mutable description: enoyl CoA hydratase (ech) was isolated from Pseudomonas fluorescens genome. Ech converts feruloyl CoA to vanillin (fig. 3) and catalyses the final stage of the vanillin biosynthesis pathway. Involved in the synthesis of 'natural' vanilla flavouring
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Gene was synthesised by geneart as we experienced problems isolating native gene, due to the high GC nature of the Pseudomonas genome. The ech sequence has been optimised for expression in E. coli
star: false
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: ech coding region
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I742112_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1949783
display id: annotation1949783
version: 1
top level: BBa_I742112
location: https://synbiohub.org/public/igem/BBa_I742112/annotation1949783/range1949783/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I742112/annotation1949783/range1949783/1
type: Range
persistent identity: range1949783
display id: range1949783
version: 1
top level: BBa_I742112
direction: direction/forward
start: 1
end: 3
orientation: inline

ech coding region
type: SequenceAnnotation
persistent identity: annotation1949782
display id: annotation1949782
version: 1
top level: BBa_I742112
location: https://synbiohub.org/public/igem/BBa_I742112/annotation1949782/range1949782/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I742112/annotation1949782/range1949782/1
type: Range
persistent identity: range1949782
display id: range1949782
version: 1
top level: BBa_I742112
direction: direction/forward
start: 1
end: 828
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1949784
display id: annotation1949784
version: 1
top level: BBa_I742112
location: https://synbiohub.org/public/igem/BBa_I742112/annotation1949784/range1949784/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I742112/annotation1949784/range1949784/1
type: Range
persistent identity: range1949784
display id: range1949784
version: 1
top level: BBa_I742112
direction: direction/forward
start: 829
end: 834
orientation: inline

https://synbiohub.org/public/igem/BBa_K238004_sequence/1
type: Sequence
persistent identity: BBa K238004 sequence
display id: BBa_K238004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagtgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatcccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcgctcgccttcaccggtgtcggcgaggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgctcgccgcgctgggggcggagccgttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcctcgcggtggctgtgctcaaccaccggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgaccctgctgggggccaatcccgagcactacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcggagtggatccggcaggggctgccccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtgcgcgccgcaggtgctcggcgcggtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgccaggacaacccgatcacctacgagggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccgaccagatcgggttggccatgcacatggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaacggcgacctgccgcccatgctcaccccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcgttcgtctcgcggatccggcagctggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacgtgccgatggcgctcaacggggcgaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggtgggcgtcgcgcagctcgcggccatgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccgctggacgccgaccgcccgctgggcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcgtcgacgaggcagacgggaaggatttcggataataatactagagacggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23078_sequence/1
type: Sequence
persistent identity: BBa J23078 sequence
display id: BBa_J23078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742107_sequence/1
type: Sequence
persistent identity: BBa I742107 sequence
display id: BBa_I742107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggttcaacaggtgaaactcaaataacaccaacccacatatcagatgaagaagcaaacctcttcgccatgcaactagcaagtgcttcagttcttcccatgattttgaaatcagctcttgaacttgatctcttagaaatcattgctaaagctggacctggtgctcaaatttcacctattgaaattgcttctcagctaccaacaactaaccctgatgcaccagttatgttggaccgaatgttgcgtctcttggcttgttacataatcctcacatgttcagttcgtactcaacaagatggaaaggttcagagactttatggtttggctactgttgctaagtatttggttaagaatgaagatggtgtatccatttctgctcttaatctcatgaatcaggataaagtgctcatggaaagctggtaccacctaaaagatgcagtccttgatgggggcattccattcaacaaggcttatggaatgacagcctttgaataccatggaacagatccaaggtttaacaaggttttcaacaaggggatgtctgatcactctaccatcacaatgaagaaaattcttgagacctacacaggttttgaaggccttaaatctcttgttgatgtaggtggtggtactggagctgtaattaacacgattgtctcaaaatatcccactataaagggtataaattttgatttaccccatgtcattgaagatgctccatcttatccaggagttgagcatgttggtggagacatgtttgtcagtattccaaaggctgatgctgtttttatgaagtggatttgtcatgactggagtgatgagcactgcttgaaatttttgaagaactgctatgaggcactgccagacaatggaaaagtgattgtggcagaatgcatacttccagtggctccagattcaagcctggccacaaaaggtgtggttcacattgatgtgatcatgttggctcataatcctggtgggaaagagagaacacaaaaagagtttgaggatcttgccaaaggtgctggattccaaggtttcaaagtccattgtaatgctttcaacacatacatcatggagtttcttaagaaggtttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742114_sequence/1
type: Sequence
persistent identity: BBa I742114 sequence
display id: BBa_I742114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtagcctggaaccgctggccgaactgccggcacgtctgctggatcgtctggtgcattgggcgcgtgtgcgtccggaacagacctttattgcggcgcgtcaggcgggtggtgattggcgtcgtgtgagctatcgcgaaatgctggatggcgtgcgtgcgattgcgcagggcctgctgtcttatggcctgagcgcggataaaccgctggccctgctgtctggcaacgatattgaacatctgcaagtggcgctgggcgcaatgtatgcgggcattccgtattgcccggtgagcccggcgtatagcctgctgtctcaggattttgcgaaactgcgtcatgtgtgcgatctgctgcaaccgggcctggtttttgtgtctgatgcgccggcatatcagcgtgcgattgatgcggttctgccgccggaaaccccgctgattagcgtgcgtggccaggttccgggccgtacgcagattagctttgcgagcctgctgcataccagcgcgggcaccgaagcggatgcggcgtttaacgcgaccggtccggatagcattgcgaaatttctgtttaccagcggcagcaccaaactgccgaaagcggtgattaccacccagcgtatgctgtgcgcgaaccagcagatgctgctgcaaacctttccggtgtttggcgaagaaccgccggtgctggttgattggctgccgtggaaccacacctttggcggcagccataacgtgggcattgtgctgtataacggcggcaccttttatctggatgatggcaaaccgaccgcccagggctttgcacagacgctgcgcaacctgaaagaaattagcccgaccgcgtatctgaccgtgccgaaaggctgggaagaactggtgaacgcgctggaacaggatgcggcgctgcgtgaacgtttttttgcgcgcatgaaactgtttttttttgcggcggcaggcctgagccagagcgtgtgggatcgtctggatcgtgtggcggaacaacattgtggcgaacgtattcgcatgatggcgggcctgggcatgaccgaagcggcaccgagctgcacctttaccaccggtccgctgtctatggcgggctatattggcctgccggcaccgggttgtgaagtgcgtctggtgccggtggatggcaaatttgaaggccgttttcgtggcccgcatatcatgccgggttattggcgtgcaccggaacagaccgcggaagtgtttgatgaacagggctattattgcagcggtgatgcgatcaaactggccgatccgcatcagccgcattggggcctgatgtttgatggccgtattgcggaagattttaaactgagcagcggcgtgtttgtgtccgtgggcccgctgcgtaatcgtgcggttctggaaggcagcccgtatgtgcaggatctggtgattgcggcaccggatcgtgaatgcctgggcgccctggtttttccgcgtgcgtatgaatgccgtcgtctgagcggcctgaacgcggaagcgtctgatgcggaagtgctgaacagcgcaccggttcgtcagtggtttggcgattggctgcaacgtctgaaccgtgaagcgaccggcaatgcgagccgtctggaatggatcgcggtgctggatgaaccggcgagcattgatcgtggcgaaattaccgataaaggcagcattaaccagcgcgcggtgctgcaatggcgtgcggcgaaagtggaagcgctgtatcgtggcgaagatccgaccattctgcgtgcgggtccgaaaccgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742142_sequence/1
type: Sequence
persistent identity: BBa I742142 sequence
display id: BBa_I742142_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatcccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcgctcgccttcaccggtgtcggcgaggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgctcgccgcgctgggggcggagccgttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcctcgcggtggctgtgctcaaccaccggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgaccctgctgggggccaatcccgagcactacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcggagtggatccggcaggggctgccccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtgcgcgccgcaggtgctcggcgcggtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgccaggacaacccgatcacctacgagggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccgaccagatcgggttggccatgcacatggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaacggcgacctgccgcccatgctcaccccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcgttcgtctcgcggatccggcagctggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacgtgccgatggcgctcaacggggcgaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggtgggcgtcgcgcagctcgcggccatgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccgctggacgccgaccgcccgctgggcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcgtcgacgaggcagacgggaaggatttcggataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23110_sequence/1
type: Sequence
persistent identity: BBa J23110 sequence
display id: BBa_J23110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742112_sequence/1
type: Sequence
persistent identity: BBa I742112 sequence
display id: BBa_I742112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaaatatgaaggccgttggaccaccgtgaaagtggaaattgaagaaggcattgcctgggttattctgaaccgtccggaaaaacgtaacgccatgagcccgaccctgaaccgtgaaatgattgatgtgctggaaaccctggaacaggatccggcggcgggtgttctggttctgaccggtgcgggtgaagcgtggaccgcgggcatggatctgaaagaatatttccgtgaagtggatgcgggtccggaaattctgcaagaaaaaattcgtcgcgaagcgagccagtggcagtggaaactgctgcgtatgtatgcgaaaccgaccattgccatggtgaacggctggtgctttggcggcggttttagcccgctggttgcgtgcgatctggccatttgcgcggatgaagcgacctttggcctgagcgagattaactggggcattccgccgggtaacctggtgagcaaagccatggcggataccgtgggccatcgtcagagcctgtattatatcatgaccggcaaaacctttggtggccagaaagcggcggaaatgggcctggtgaacgaaagcgtgccgctggcccagctgcgtgaagtgaccattgaactggcccgtaacctgctggaaaaaaatccggtggtgctgcgtgcggcgaaacatggctttaaacgttgccgtgaactgacctgggaacagaacgaagattatctgtacgcgaaactggatcagagccgtctgctggataccgaaggcggccgtgaacagggcatgaaacagtttctggatgataaaagcattaaaccgggcctgcaagcgtataaacgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K238007_sequence/1
type: Sequence
persistent identity: BBa K238007 sequence
display id: BBa_K238007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcatccgggtgcagaccctgcaagcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z