BBa_K238006
type: ComponentDefinition
persistent identity: BBa K238006
display id: BBa_K238006
version: 1
was generated by: iGEM to SBOL conversion
description: Short version of vanillin synthesis
created: 2009-07-15T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: makes vanillin from ferulic acid
discontinued: false
dominant: false
group u list: _332_
m user id: 0
owner id: 4771
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: Annelien Verfaillie
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23110
component: BBa_J15001
component: BBa_J23078
component: BBa_I742114
component: BBa_I742112
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J23110
sequence annotation: BBa_J23078
sequence annotation: BBa_J15001
sequence annotation: BBa_I742114
sequence annotation: BBa_I742112
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K238006_sequence/1

BBa_J23110
type: Component
persistent identity: component2013532
display id: component2013532
version: 1
top level: BBa_K238006
definition: BBa_J23110
access: public

BBa_J15001
type: Component
persistent identity: component2013536
display id: component2013536
version: 1
top level: BBa_K238006
definition: BBa_J15001
access: public

BBa_J23078
type: Component
persistent identity: component2013533
display id: component2013533
version: 1
top level: BBa_K238006
definition: BBa_J23078
access: public

BBa_J23078
type: Component
persistent identity: component2013539
display id: component2013539
version: 1
top level: BBa_K238006
definition: BBa_J23078
access: public

BBa_I742114
type: Component
persistent identity: component2013538
display id: component2013538
version: 1
top level: BBa_K238006
definition: BBa_I742114
access: public

BBa_I742112
type: Component
persistent identity: component2013546
display id: component2013546
version: 1
top level: BBa_K238006
definition: BBa_I742112
access: public

BBa_J15001
type: Component
persistent identity: component2013542
display id: component2013542
version: 1
top level: BBa_K238006
definition: BBa_J15001
access: public

BBa_B0012
type: Component
persistent identity: component2013549
display id: component2013549
version: 1
top level: BBa_K238006
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2013547
display id: component2013547
version: 1
top level: BBa_K238006
definition: BBa_B0010
access: public

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2013532
display id: annotation2013532
version: 1
top level: BBa_K238006
location: https://synbiohub.org/public/igem/BBa_K238006/annotation2013532/range2013532/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K238006/annotation2013532/range2013532/1
type: Range
persistent identity: range2013532
display id: range2013532
version: 1
top level: BBa_K238006
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_J23078
type: SequenceAnnotation
persistent identity: annotation2013533
display id: annotation2013533
version: 1
top level: BBa_K238006
location: https://synbiohub.org/public/igem/BBa_K238006/annotation2013533/range2013533/1
component: BBa_J23078

https://synbiohub.org/public/igem/BBa_K238006/annotation2013533/range2013533/1
type: Range
persistent identity: range2013533
display id: range2013533
version: 1
top level: BBa_K238006
direction: direction/reverse
start: 44
end: 95
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2013536
display id: annotation2013536
version: 1
top level: BBa_K238006
location: https://synbiohub.org/public/igem/BBa_K238006/annotation2013536/range2013536/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K238006/annotation2013536/range2013536/1
type: Range
persistent identity: range2013536
display id: range2013536
version: 1
top level: BBa_K238006
direction: direction/reverse
start: 104
end: 113
orientation: inline

BBa_I742114
type: SequenceAnnotation
persistent identity: annotation2013538
display id: annotation2013538
version: 1
top level: BBa_K238006
location: https://synbiohub.org/public/igem/BBa_K238006/annotation2013538/range2013538/1
component: BBa_I742114

https://synbiohub.org/public/igem/BBa_K238006/annotation2013538/range2013538/1
type: Range
persistent identity: range2013538
display id: range2013538
version: 1
top level: BBa_K238006
direction: direction/reverse
start: 120
end: 1892
orientation: inline

BBa_J23078
type: SequenceAnnotation
persistent identity: annotation2013539
display id: annotation2013539
version: 1
top level: BBa_K238006
location: https://synbiohub.org/public/igem/BBa_K238006/annotation2013539/range2013539/1
component: BBa_J23078

https://synbiohub.org/public/igem/BBa_K238006/annotation2013539/range2013539/1
type: Range
persistent identity: range2013539
display id: range2013539
version: 1
top level: BBa_K238006
direction: direction/reverse
start: 1901
end: 1952
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2013542
display id: annotation2013542
version: 1
top level: BBa_K238006
location: https://synbiohub.org/public/igem/BBa_K238006/annotation2013542/range2013542/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K238006/annotation2013542/range2013542/1
type: Range
persistent identity: range2013542
display id: range2013542
version: 1
top level: BBa_K238006
direction: direction/reverse
start: 1961
end: 1970
orientation: inline

BBa_I742112
type: SequenceAnnotation
persistent identity: annotation2013546
display id: annotation2013546
version: 1
top level: BBa_K238006
location: https://synbiohub.org/public/igem/BBa_K238006/annotation2013546/range2013546/1
component: BBa_I742112

https://synbiohub.org/public/igem/BBa_K238006/annotation2013546/range2013546/1
type: Range
persistent identity: range2013546
display id: range2013546
version: 1
top level: BBa_K238006
direction: direction/reverse
start: 1977
end: 2810
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2013547
display id: annotation2013547
version: 1
top level: BBa_K238006
location: https://synbiohub.org/public/igem/BBa_K238006/annotation2013547/range2013547/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K238006/annotation2013547/range2013547/1
type: Range
persistent identity: range2013547
display id: range2013547
version: 1
top level: BBa_K238006
direction: direction/forward
start: 2819
end: 2898
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2013549
display id: annotation2013549
version: 1
top level: BBa_K238006
location: https://synbiohub.org/public/igem/BBa_K238006/annotation2013549/range2013549/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K238006/annotation2013549/range2013549/1
type: Range
persistent identity: range2013549
display id: range2013549
version: 1
top level: BBa_K238006
direction: direction/forward
start: 2907
end: 2947
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23078
type: ComponentDefinition
persistent identity: BBa J23078
display id: BBa_J23078
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3i]
created: 2006-09-12T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: PCR ca1028F/G00101 on pJ23006-J23032 . . . (162 bp, XbaI/PstI) Sub into pSB1A2-I13521 . . . . . . . . . . (XbaI/PstI) Product is pSB1A2-J23078
owned by: james
owned by: myers
mutable description: lock3d derivative with a 5' extension. This is the basic part, J23077 is the RFP reporter.
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23078_sequence/1

BBa_I742112
type: ComponentDefinition
persistent identity: BBa I742112
display id: BBa_I742112
version: 1
was generated by: iGEM to SBOL conversion
description: enoyl CoA hydratase (ech) gene
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Pseudomonas fluorescens NCIMB 9046 Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
owned by: james
owned by: myers
mutable description: enoyl CoA hydratase (ech) was isolated from Pseudomonas fluorescens genome. Ech converts feruloyl CoA to vanillin (fig. 3) and catalyses the final stage of the vanillin biosynthesis pathway. Involved in the synthesis of 'natural' vanilla flavouring
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Gene was synthesised by geneart as we experienced problems isolating native gene, due to the high GC nature of the Pseudomonas genome. The ech sequence has been optimised for expression in E. coli
star: false
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: ech coding region
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I742112_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1949783
display id: annotation1949783
version: 1
top level: BBa_I742112
location: https://synbiohub.org/public/igem/BBa_I742112/annotation1949783/range1949783/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I742112/annotation1949783/range1949783/1
type: Range
persistent identity: range1949783
display id: range1949783
version: 1
top level: BBa_I742112
direction: direction/forward
start: 1
end: 3
orientation: inline

ech coding region
type: SequenceAnnotation
persistent identity: annotation1949782
display id: annotation1949782
version: 1
top level: BBa_I742112
location: https://synbiohub.org/public/igem/BBa_I742112/annotation1949782/range1949782/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I742112/annotation1949782/range1949782/1
type: Range
persistent identity: range1949782
display id: range1949782
version: 1
top level: BBa_I742112
direction: direction/forward
start: 1
end: 828
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1949784
display id: annotation1949784
version: 1
top level: BBa_I742112
location: https://synbiohub.org/public/igem/BBa_I742112/annotation1949784/range1949784/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I742112/annotation1949784/range1949784/1
type: Range
persistent identity: range1949784
display id: range1949784
version: 1
top level: BBa_I742112
direction: direction/forward
start: 829
end: 834
orientation: inline

BBa_I742114
type: ComponentDefinition
persistent identity: BBa I742114
display id: BBa_I742114
version: 1
was generated by: iGEM to SBOL conversion
description: Feruloyl-CoA synthase (fcs)
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Pseudomonas fluorescens NCIMB 9046 Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
owned by: james
owned by: myers
mutable description: gene is from Pseudomonas fluorescens encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid) produces feruloyl CoA from ferrulic acid catalyses the penultimate of the vanillin biosynthesis pathway
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Gene was synthesised by geneart, as P. fluorescens has a high GC genome. Coding region was optimised for gene expression in E. coli
star: false
creator: sarah hollingshead
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: fcs coding region
sequence: https://synbiohub.org/public/igem/BBa_I742114_sequence/1

fcs coding region
type: SequenceAnnotation
persistent identity: annotation1949792
display id: annotation1949792
version: 1
top level: BBa_I742114
location: https://synbiohub.org/public/igem/BBa_I742114/annotation1949792/range1949792/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I742114/annotation1949792/range1949792/1
type: Range
persistent identity: range1949792
display id: range1949792
version: 1
top level: BBa_I742114
direction: direction/forward
start: 1
end: 1773
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

https://synbiohub.org/public/igem/BBa_K238006_sequence/1
type: Sequence
persistent identity: BBa K238006 sequence
display id: BBa_K238006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagtgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggagatactagagctcaaggaggtactagatgcgtagcctggaaccgctggccgaactgccggcacgtctgctggatcgtctggtgcattgggcgcgtgtgcgtccggaacagacctttattgcggcgcgtcaggcgggtggtgattggcgtcgtgtgagctatcgcgaaatgctggatggcgtgcgtgcgattgcgcagggcctgctgtcttatggcctgagcgcggataaaccgctggccctgctgtctggcaacgatattgaacatctgcaagtggcgctgggcgcaatgtatgcgggcattccgtattgcccggtgagcccggcgtatagcctgctgtctcaggattttgcgaaactgcgtcatgtgtgcgatctgctgcaaccgggcctggtttttgtgtctgatgcgccggcatatcagcgtgcgattgatgcggttctgccgccggaaaccccgctgattagcgtgcgtggccaggttccgggccgtacgcagattagctttgcgagcctgctgcataccagcgcgggcaccgaagcggatgcggcgtttaacgcgaccggtccggatagcattgcgaaatttctgtttaccagcggcagcaccaaactgccgaaagcggtgattaccacccagcgtatgctgtgcgcgaaccagcagatgctgctgcaaacctttccggtgtttggcgaagaaccgccggtgctggttgattggctgccgtggaaccacacctttggcggcagccataacgtgggcattgtgctgtataacggcggcaccttttatctggatgatggcaaaccgaccgcccagggctttgcacagacgctgcgcaacctgaaagaaattagcccgaccgcgtatctgaccgtgccgaaaggctgggaagaactggtgaacgcgctggaacaggatgcggcgctgcgtgaacgtttttttgcgcgcatgaaactgtttttttttgcggcggcaggcctgagccagagcgtgtgggatcgtctggatcgtgtggcggaacaacattgtggcgaacgtattcgcatgatggcgggcctgggcatgaccgaagcggcaccgagctgcacctttaccaccggtccgctgtctatggcgggctatattggcctgccggcaccgggttgtgaagtgcgtctggtgccggtggatggcaaatttgaaggccgttttcgtggcccgcatatcatgccgggttattggcgtgcaccggaacagaccgcggaagtgtttgatgaacagggctattattgcagcggtgatgcgatcaaactggccgatccgcatcagccgcattggggcctgatgtttgatggccgtattgcggaagattttaaactgagcagcggcgtgtttgtgtccgtgggcccgctgcgtaatcgtgcggttctggaaggcagcccgtatgtgcaggatctggtgattgcggcaccggatcgtgaatgcctgggcgccctggtttttccgcgtgcgtatgaatgccgtcgtctgagcggcctgaacgcggaagcgtctgatgcggaagtgctgaacagcgcaccggttcgtcagtggtttggcgattggctgcaacgtctgaaccgtgaagcgaccggcaatgcgagccgtctggaatggatcgcggtgctggatgaaccggcgagcattgatcgtggcgaaattaccgataaaggcagcattaaccagcgcgcggtgctgcaatggcgtgcggcgaaagtggaagcgctgtatcgtggcgaagatccgaccattctgcgtgcgggtccgaaaccgtaataatactagagtgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggagatactagagctcaaggaggtactagatgagcaaatatgaaggccgttgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23078_sequence/1
type: Sequence
persistent identity: BBa J23078 sequence
display id: BBa_J23078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742112_sequence/1
type: Sequence
persistent identity: BBa I742112 sequence
display id: BBa_I742112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaaatatgaaggccgttggaccaccgtgaaagtggaaattgaagaaggcattgcctgggttattctgaaccgtccggaaaaacgtaacgccatgagcccgaccctgaaccgtgaaatgattgatgtgctggaaaccctggaacaggatccggcggcgggtgttctggttctgaccggtgcgggtgaagcgtggaccgcgggcatggatctgaaagaatatttccgtgaagtggatgcgggtccggaaattctgcaagaaaaaattcgtcgcgaagcgagccagtggcagtggaaactgctgcgtatgtatgcgaaaccgaccattgccatggtgaacggctggtgctttggcggcggttttagcccgctggttgcgtgcgatctggccatttgcgcggatgaagcgacctttggcctgagcgagattaactggggcattccgccgggtaacctggtgagcaaagccatggcggataccgtgggccatcgtcagagcctgtattatatcatgaccggcaaaacctttggtggccagaaagcggcggaaatgggcctggtgaacgaaagcgtgccgctggcccagctgcgtgaagtgaccattgaactggcccgtaacctgctggaaaaaaatccggtggtgctgcgtgcggcgaaacatggctttaaacgttgccgtgaactgacctgggaacagaacgaagattatctgtacgcgaaactggatcagagccgtctgctggataccgaaggcggccgtgaacagggcatgaaacagtttctggatgataaaagcattaaaccgggcctgcaagcgtataaacgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742114_sequence/1
type: Sequence
persistent identity: BBa I742114 sequence
display id: BBa_I742114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtagcctggaaccgctggccgaactgccggcacgtctgctggatcgtctggtgcattgggcgcgtgtgcgtccggaacagacctttattgcggcgcgtcaggcgggtggtgattggcgtcgtgtgagctatcgcgaaatgctggatggcgtgcgtgcgattgcgcagggcctgctgtcttatggcctgagcgcggataaaccgctggccctgctgtctggcaacgatattgaacatctgcaagtggcgctgggcgcaatgtatgcgggcattccgtattgcccggtgagcccggcgtatagcctgctgtctcaggattttgcgaaactgcgtcatgtgtgcgatctgctgcaaccgggcctggtttttgtgtctgatgcgccggcatatcagcgtgcgattgatgcggttctgccgccggaaaccccgctgattagcgtgcgtggccaggttccgggccgtacgcagattagctttgcgagcctgctgcataccagcgcgggcaccgaagcggatgcggcgtttaacgcgaccggtccggatagcattgcgaaatttctgtttaccagcggcagcaccaaactgccgaaagcggtgattaccacccagcgtatgctgtgcgcgaaccagcagatgctgctgcaaacctttccggtgtttggcgaagaaccgccggtgctggttgattggctgccgtggaaccacacctttggcggcagccataacgtgggcattgtgctgtataacggcggcaccttttatctggatgatggcaaaccgaccgcccagggctttgcacagacgctgcgcaacctgaaagaaattagcccgaccgcgtatctgaccgtgccgaaaggctgggaagaactggtgaacgcgctggaacaggatgcggcgctgcgtgaacgtttttttgcgcgcatgaaactgtttttttttgcggcggcaggcctgagccagagcgtgtgggatcgtctggatcgtgtggcggaacaacattgtggcgaacgtattcgcatgatggcgggcctgggcatgaccgaagcggcaccgagctgcacctttaccaccggtccgctgtctatggcgggctatattggcctgccggcaccgggttgtgaagtgcgtctggtgccggtggatggcaaatttgaaggccgttttcgtggcccgcatatcatgccgggttattggcgtgcaccggaacagaccgcggaagtgtttgatgaacagggctattattgcagcggtgatgcgatcaaactggccgatccgcatcagccgcattggggcctgatgtttgatggccgtattgcggaagattttaaactgagcagcggcgtgtttgtgtccgtgggcccgctgcgtaatcgtgcggttctggaaggcagcccgtatgtgcaggatctggtgattgcggcaccggatcgtgaatgcctgggcgccctggtttttccgcgtgcgtatgaatgccgtcgtctgagcggcctgaacgcggaagcgtctgatgcggaagtgctgaacagcgcaccggttcgtcagtggtttggcgattggctgcaacgtctgaaccgtgaagcgaccggcaatgcgagccgtctggaatggatcgcggtgctggatgaaccggcgagcattgatcgtggcgaaattaccgataaaggcagcattaaccagcgcgcggtgctgcaatggcgtgcggcgaaagtggaagcgctgtatcgtggcgaagatccgaccattctgcgtgcgggtccgaaaccgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23110_sequence/1
type: Sequence
persistent identity: BBa J23110 sequence
display id: BBa_J23110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z