LOCUS BBa_K238012 4465 bp DNA linear 25-Nov-2024 DEFINITION short version II of vanillin synthesis ACCESSION BBa_K238012 VERSION BBa_K238012.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 misc_feature 44..95 /label=BBa_J23078 CDS 102..1637 /label=BBa_I742142 RBS 1646..1661 /label=rbs CDS 1662..3203 /label=sam5 misc_feature 1646..3203 /label=BBa_K238007 misc_feature 3212..3214 /label=SacI RBS 3215..3221 /label=rbs RBS 3212..3221 /label=BBa_J15001 CDS 3228..4328 /label=COMT coding region CDS 3228..4328 /label=BBa_I742107 stem_loop 4348..4391 /label=stem_loop misc_feature 4337..4416 /label=BBa_B0010 terminator 4337..4416 /label=BBa_B0010 stem_loop 4432..4451 /label=T7 TE misc_feature 4458..4458 /label=stop polyA_site 4452..4465 /label=polya misc_feature 4425..4465 /label=BBa_B0012 terminator 4425..4465 /label=BBa_B0012 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagtgtagac cgaactagaa 61 tcacctcttg cttttgggta agacagaaga ggagatacta gatgacgcag gtcgtggaac 121 gtcaggctga tcggctcagc agcagggagt acctggcccg ggtcgtgcgc agcgccgggt 181 gggacgccgg tctcacctcg tgcaccgacg aggagatcgt ccggatgggc gcgagcgcgc 241 gcaccatcga ggagtacctg aagtccgaca agcccatcta cggcctgacg cagggcttcg 301 gtccgctggt gctgttcgac gccgactcgg agctggagca gggcggctcg ctgatctcgc 361 acctgggcac cggccagggc gcgccactgg ccccggaggt gtcgcggctg atcctctggc 421 tgcgcatcca gaacatgcgc aaggggtact cggcggtctc gccggtgttc tggcagaagc 481 tcgccgacct gtggaacaag gggttcaccc cggcgatccc ccggcacggc acggtcagcg 541 cgagcggcga cctgcaaccg ctggcgcacg ccgcgctcgc cttcaccggt gtcggcgagg 601 cgtggacccg ggacgccgac ggccggtggt ccaccgtgcc ggccgtggac gcgctcgccg 661 cgctgggggc ggagccgttc gactggccgg tgcgcgaggc gctggcgttc gtcaacggga 721 ccggcgcgag cctcgcggtg gctgtgctca accaccggtc cgccctgcgg ctggtccgcg 781 cctgcgccgt gctctccgcg cggctggcga ccctgctggg ggccaatccc gagcactacg 841 acgtggggca cggtgtcgcg cgcggccagg tcggtcagct gaccgcggcg gagtggatcc 901 ggcaggggct gccccggggc atggtgcgcg acggcagccg cccgctccag gagccgtaca 961 gcctgcggtg cgcgccgcag gtgctcggcg cggtgctcga ccagctcgac ggcgcgggcg 1021 acgtgctggc gcgggaggtc gacggctgcc aggacaaccc gatcacctac gagggcgagc 1081 tgctgcacgg cggcaacttc cacgccatgc cggtgggttt cgcctccgac cagatcgggt 1141 tggccatgca catggccgcc tacctggccg agcgccagct gggtctgctg gtcagcccgg 1201 tgaccaacgg cgacctgccg cccatgctca ccccgcgcgc cgggcgcggt gccgggctgg 1261 ccggggtgca gatcagcgcg acctcgttcg tctcgcggat ccggcagctg gtgttccccg 1321 cctcgctgac caccctgccg accaacggct ggaaccagga ccacgtgccg atggcgctca 1381 acggggcgaa ctcggtgttc gaggcgttgg agctcggctg gctgacggtc gggtcgctgg 1441 cggtgggcgt cgcgcagctc gcggccatga ccggccacgc cgcggagggc gtctgggcgg 1501 agctggccgg gatctgcccg ccgctggacg ccgaccgccc gctgggcgcc gaggtgcgcg 1561 ccgcgcgcga cctgctgtcc gcgcacgcgg accaactgct cgtcgacgag gcagacggga 1621 aggatttcgg ataataatac tagagacgga gaagcagcga aatgaccatc acgtcacctg 1681 cgccggcggg ccggctcaac aacgtccgcc cgatgacggg cgaggagtac ctggaatcac 1741 tgcgggacgg ccgagaggtc tacatctacg gcgagcgggt cgacgacgtc accacgcacc 1801 tggccttccg caacagcgtg cgctccatcg cgcggctcta cgacgtgctg cacgacccgg 1861 cgtccgaagg tgtgctgcgg gtgcccaccg acaccggcaa cggcgggttc acccacccgt 1921 tcttcaagac cgcccggtcg tcggaggacc tggtcgccgc gcgcgaggcc atcgtcggct 1981 ggcagcggct ggtgtacggg tggatgggcc gcaccccgga ctacaaggcg gcgttcttcg 2041 gcacgctcga cgccaacgcc gagttctacg ggccgttcga ggccaacgcc cgccgctggt 2101 accgcgacgc ccaggaacgg gtgctgtact tcaaccacgc gatcgtgcac ccgccggtcg 2161 accgggaccg gcccgccgac cggaccgccg acatctgcgt gcacgtggag gaggagaccg 2221 acagcgggtt gatcgtctcc ggcgccaagg tggtcgcgac cggctccgcg atgaccaacg 2281 cgaacctcat cgcgcactac gggcttccgg tgcgggacaa gaagttcggc ctggtgttca 2341 cggtcccgat gaactcgccc ggcctcaagc tcatctgccg cacctcctac gagctgatgg 2401 tcgcgacgca gggctcgccc ttcgactacc cgctgtcgag ccggctcgac gagaacgact 2461 cgatcatgat cttcgaccgg gtgctggtgc cctgggagaa cgtgttcatg tacgacgcgg 2521 gcgcggccaa ctccttcgcc accgggtcag gcttcctcga acgcttcacc ttccacggct 2581 gcacccgcct cgcggtcaag ctggacttca tcgccggctg cgtcatgaag gcggtggagg 2641 tcaccggcac cacgcacttc cggggcgtgc aggcgcaggt cggcgaagtg ctcaactggc 2701 gcgacgtgtt ctggggcctg tccgacgcga tggccaagtc gccgaactcg tgggtcggcg 2761 gctcggtgca gccgaacctc aactacgggc tcgcgtaccg caccttcatg ggcgtgggct 2821 acccgcgcat caaggagatc atccagcaga ccctcggcag cgggttgatc tacctgaact 2881 cctcggccgc cgactggaag aaccccgacg tccgcccgta cctcgaccgc tacctgcgcg 2941 gctcgcgggg catccaggcg atcgaccggg tcaagctgct gaagctgctg tgggacgcgg 3001 tcggcaccga gttcgccggc cggcacgagc tctacgagcg caactacggc ggcgaccacg 3061 agggcatccg ggtgcagacc ctgcaagcgt accaggcgaa cggccaagcc gccgcgctca 3121 agggtttcgc cgagcagtgc atgtccgagt acgacctcga cggctggacg aggcccgacc 3181 tgatcaaccc cggcacctaa taatactaga gctcaaggag gtactagatg ggttcaacag 3241 gtgaaactca aataacacca acccacatat cagatgaaga agcaaacctc ttcgccatgc 3301 aactagcaag tgcttcagtt cttcccatga ttttgaaatc agctcttgaa cttgatctct 3361 tagaaatcat tgctaaagct ggacctggtg ctcaaatttc acctattgaa attgcttctc 3421 agctaccaac aactaaccct gatgcaccag ttatgttgga ccgaatgttg cgtctcttgg 3481 cttgttacat aatcctcaca tgttcagttc gtactcaaca agatggaaag gttcagagac 3541 tttatggttt ggctactgtt gctaagtatt tggttaagaa tgaagatggt gtatccattt 3601 ctgctcttaa tctcatgaat caggataaag tgctcatgga aagctggtac cacctaaaag 3661 atgcagtcct tgatgggggc attccattca acaaggctta tggaatgaca gcctttgaat 3721 accatggaac agatccaagg tttaacaagg ttttcaacaa ggggatgtct gatcactcta 3781 ccatcacaat gaagaaaatt cttgagacct acacaggttt tgaaggcctt aaatctcttg 3841 ttgatgtagg tggtggtact ggagctgtaa ttaacacgat tgtctcaaaa tatcccacta 3901 taaagggtat aaattttgat ttaccccatg tcattgaaga tgctccatct tatccaggag 3961 ttgagcatgt tggtggagac atgtttgtca gtattccaaa ggctgatgct gtttttatga 4021 agtggatttg tcatgactgg agtgatgagc actgcttgaa atttttgaag aactgctatg 4081 aggcactgcc agacaatgga aaagtgattg tggcagaatg catacttcca gtggctccag 4141 attcaagcct ggccacaaaa ggtgtggttc acattgatgt gatcatgttg gctcataatc 4201 ctggtgggaa agagagaaca caaaaagagt ttgaggatct tgccaaaggt gctggattcc 4261 aaggtttcaa agtccattgt aatgctttca acacatacat catggagttt cttaagaagg 4321 tttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 4381 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 4441 tcgggtgggc ctttctgcgt ttata //