LOCUS       BBa_K238015              970 bp    DNA     linear       05-May-2024
DEFINITION  blue light promoter and GFP
ACCESSION   BBa_K238015
VERSION     BBa_K238015.1  
FEATURES             Location/Qualifiers
     promoter        28..33
                     /label=-35 box
     promoter        52..58
                     /label=-10 box
     misc_feature    34..51
                     /label=spacer
     misc_feature    53..59
                     /label=inverted repeat part 1
     misc_feature    67..73
                     /label=inverted repeat part 2
     misc_feature    64..65
                     /label=transcription start
     promoter        1..86
                     /label=BBa_K238013
     RBS             95..107
                     /label=RBS-3\Weak
     RBS             101..104
                     /label=RBS
     misc_feature    95..107
                     /label=BBa_B0032
     RBS             95..107
                     /label=BBa_B0032
     CDS             114..833
                     /label=GFP protein
     CDS             114..833
                     /label=BBa_E0040
     stem_loop       853..896
                     /label=stem_loop
     misc_feature    842..921
                     /label=BBa_B0010
     terminator      842..921
                     /label=BBa_B0010
     stem_loop       937..956
                     /label=T7 TE
     misc_feature    963..963
                     /label=stop
     polyA_site      957..970
                     /label=polya
     misc_feature    930..970
                     /label=BBa_B0012
     terminator      930..970
                     /label=BBa_B0012
ORIGIN
        1 attgcaaaaa attaatttat cattctgtac acatatttcg tacaagtttg ctattgttac
       61 ttcacttaac attgattaac attttttact agagtcacac aggaaagtac tagatgcgta
      121 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      181 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      241 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      301 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      361 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      421 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      481 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      541 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      601 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      661 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      721 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
      781 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
      841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      961 tgcgtttata
//