LOCUS       BBa_K238016              955 bp    DNA     linear       02-May-2024
DEFINITION  blue light promoter and RFP
ACCESSION   BBa_K238016
VERSION     BBa_K238016.1  
FEATURES             Location/Qualifiers
     promoter        28..33
                     /label=-35 box
     promoter        52..58
                     /label=-10 box
     misc_feature    34..51
                     /label=spacer
     misc_feature    53..59
                     /label=inverted repeat part 1
     misc_feature    67..73
                     /label=inverted repeat part 2
     misc_feature    64..65
                     /label=transcription start
     promoter        1..86
                     /label=BBa_K238013
     misc_feature    99..102
                     /label=conserved
     RBS             95..106
                     /label=BBa_B0034
     CDS             113..787
                     /label=mrfp1
     misc_feature    794..818
                     /label=Help:Barcodes
     CDS             113..818
                     /label=BBa_E1010
     stem_loop       838..881
                     /label=stem_loop
     misc_feature    827..906
                     /label=BBa_B0010
     terminator      827..906
                     /label=BBa_B0010
     stem_loop       922..941
                     /label=T7 TE
     misc_feature    948..948
                     /label=stop
     polyA_site      942..955
                     /label=polya
     misc_feature    915..955
                     /label=BBa_B0012
     terminator      915..955
                     /label=BBa_B0012
     terminator      827..955
                     /label=BBa_B0015
ORIGIN
        1 attgcaaaaa attaatttat cattctgtac acatatttcg tacaagtttg ctattgttac
       61 ttcacttaac attgattaac attttttact agagaaagag gagaaatact agatggcttc
      121 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
      181 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
      241 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
      301 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
      361 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
      421 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
      481 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
      541 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
      601 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
      661 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
      721 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
      781 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
      841 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      901 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//