LOCUS BBa_K238017 2784 bp DNA linear 01-Jun-2024 DEFINITION EF ligation with terminator ACCESSION BBa_K238017 VERSION BBa_K238017.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=SacI RBS 4..10 /label=rbs RBS 1..10 /label=BBa_J15001 CDS 17..1789 /label=fcs coding region CDS 17..1789 /label=BBa_I742114 misc_feature 1798..1800 /label=SacI RBS 1801..1807 /label=rbs RBS 1798..1807 /label=BBa_J15001 CDS 1814..2641 /label=ech coding region misc_feature 1814..1816 /label=start misc_feature 2642..2647 /label=stop CDS 1814..2647 /label=BBa_I742112 stem_loop 2667..2710 /label=stem_loop misc_feature 2656..2735 /label=BBa_B0010 terminator 2656..2735 /label=BBa_B0010 stem_loop 2751..2770 /label=T7 TE misc_feature 2777..2777 /label=stop polyA_site 2771..2784 /label=polya misc_feature 2744..2784 /label=BBa_B0012 terminator 2744..2784 /label=BBa_B0012 ORIGIN 1 ctcaaggagg tactagatgc gtagcctgga accgctggcc gaactgccgg cacgtctgct 61 ggatcgtctg gtgcattggg cgcgtgtgcg tccggaacag acctttattg cggcgcgtca 121 ggcgggtggt gattggcgtc gtgtgagcta tcgcgaaatg ctggatggcg tgcgtgcgat 181 tgcgcagggc ctgctgtctt atggcctgag cgcggataaa ccgctggccc tgctgtctgg 241 caacgatatt gaacatctgc aagtggcgct gggcgcaatg tatgcgggca ttccgtattg 301 cccggtgagc ccggcgtata gcctgctgtc tcaggatttt gcgaaactgc gtcatgtgtg 361 cgatctgctg caaccgggcc tggtttttgt gtctgatgcg ccggcatatc agcgtgcgat 421 tgatgcggtt ctgccgccgg aaaccccgct gattagcgtg cgtggccagg ttccgggccg 481 tacgcagatt agctttgcga gcctgctgca taccagcgcg ggcaccgaag cggatgcggc 541 gtttaacgcg accggtccgg atagcattgc gaaatttctg tttaccagcg gcagcaccaa 601 actgccgaaa gcggtgatta ccacccagcg tatgctgtgc gcgaaccagc agatgctgct 661 gcaaaccttt ccggtgtttg gcgaagaacc gccggtgctg gttgattggc tgccgtggaa 721 ccacaccttt ggcggcagcc ataacgtggg cattgtgctg tataacggcg gcacctttta 781 tctggatgat ggcaaaccga ccgcccaggg ctttgcacag acgctgcgca acctgaaaga 841 aattagcccg accgcgtatc tgaccgtgcc gaaaggctgg gaagaactgg tgaacgcgct 901 ggaacaggat gcggcgctgc gtgaacgttt ttttgcgcgc atgaaactgt ttttttttgc 961 ggcggcaggc ctgagccaga gcgtgtggga tcgtctggat cgtgtggcgg aacaacattg 1021 tggcgaacgt attcgcatga tggcgggcct gggcatgacc gaagcggcac cgagctgcac 1081 ctttaccacc ggtccgctgt ctatggcggg ctatattggc ctgccggcac cgggttgtga 1141 agtgcgtctg gtgccggtgg atggcaaatt tgaaggccgt tttcgtggcc cgcatatcat 1201 gccgggttat tggcgtgcac cggaacagac cgcggaagtg tttgatgaac agggctatta 1261 ttgcagcggt gatgcgatca aactggccga tccgcatcag ccgcattggg gcctgatgtt 1321 tgatggccgt attgcggaag attttaaact gagcagcggc gtgtttgtgt ccgtgggccc 1381 gctgcgtaat cgtgcggttc tggaaggcag cccgtatgtg caggatctgg tgattgcggc 1441 accggatcgt gaatgcctgg gcgccctggt ttttccgcgt gcgtatgaat gccgtcgtct 1501 gagcggcctg aacgcggaag cgtctgatgc ggaagtgctg aacagcgcac cggttcgtca 1561 gtggtttggc gattggctgc aacgtctgaa ccgtgaagcg accggcaatg cgagccgtct 1621 ggaatggatc gcggtgctgg atgaaccggc gagcattgat cgtggcgaaa ttaccgataa 1681 aggcagcatt aaccagcgcg cggtgctgca atggcgtgcg gcgaaagtgg aagcgctgta 1741 tcgtggcgaa gatccgacca ttctgcgtgc gggtccgaaa ccgtaataat actagagctc 1801 aaggaggtac tagatgagca aatatgaagg ccgttggacc accgtgaaag tggaaattga 1861 agaaggcatt gcctgggtta ttctgaaccg tccggaaaaa cgtaacgcca tgagcccgac 1921 cctgaaccgt gaaatgattg atgtgctgga aaccctggaa caggatccgg cggcgggtgt 1981 tctggttctg accggtgcgg gtgaagcgtg gaccgcgggc atggatctga aagaatattt 2041 ccgtgaagtg gatgcgggtc cggaaattct gcaagaaaaa attcgtcgcg aagcgagcca 2101 gtggcagtgg aaactgctgc gtatgtatgc gaaaccgacc attgccatgg tgaacggctg 2161 gtgctttggc ggcggtttta gcccgctggt tgcgtgcgat ctggccattt gcgcggatga 2221 agcgaccttt ggcctgagcg agattaactg gggcattccg ccgggtaacc tggtgagcaa 2281 agccatggcg gataccgtgg gccatcgtca gagcctgtat tatatcatga ccggcaaaac 2341 ctttggtggc cagaaagcgg cggaaatggg cctggtgaac gaaagcgtgc cgctggccca 2401 gctgcgtgaa gtgaccattg aactggcccg taacctgctg gaaaaaaatc cggtggtgct 2461 gcgtgcggcg aaacatggct ttaaacgttg ccgtgaactg acctgggaac agaacgaaga 2521 ttatctgtac gcgaaactgg atcagagccg tctgctggat accgaaggcg gccgtgaaca 2581 gggcatgaaa cagtttctgg atgataaaag cattaaaccg ggcctgcaag cgtataaacg 2641 ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2701 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 2761 cgggtgggcc tttctgcgtt tata //