LOCUS       BBa_K238021             4284 bp    DNA     linear       14-Nov-2024
DEFINITION  promoter + sam8 + sam5 + COMT
ACCESSION   BBa_K238021
VERSION     BBa_K238021.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             44..57
                     /label=rbs
     CDS             58..1593
                     /label=sam8
     misc_feature    44..1593
                     /label=BBa_K238027
     RBS             1602..1617
                     /label=rbs
     CDS             1618..3159
                     /label=sam5
     misc_feature    1602..3159
                     /label=BBa_K238007
     misc_feature    44..3159
                     /label=BBa_K238019
     misc_feature    1..3159
                     /label=BBa_K238020
     misc_feature    3168..3170
                     /label=SacI
     RBS             3171..3177
                     /label=rbs
     RBS             3168..3177
                     /label=BBa_J15001
     CDS             3184..4284
                     /label=COMT coding region
     CDS             3184..4284
                     /label=BBa_I742107
     misc_feature    3168..4284
                     /label=BBa_I742109
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gaggaggttg ggacaagatg
       61 acgcaggtcg tggaacgtca ggctgatcgg ctcagcagca gggagtacct ggcccgggtc
      121 gtgcgcagcg ccgggtggga cgccggtctc acctcgtgca ccgacgagga gatcgtccgg
      181 atgggcgcga gcgcgcgcac catcgaggag tacctgaagt ccgacaagcc catctacggc
      241 ctgacgcagg gcttcggtcc gctggtgctg ttcgacgccg actcggagct ggagcagggc
      301 ggctcgctga tctcgcacct gggcaccggc cagggcgcgc cactggcccc ggaggtgtcg
      361 cggctgatcc tctggctgcg catccagaac atgcgcaagg ggtactcggc ggtctcgccg
      421 gtgttctggc agaagctcgc cgacctgtgg aacaaggggt tcaccccggc gatcccccgg
      481 cacggcacgg tcagcgcgag cggcgacctg caaccgctgg cgcacgccgc gctcgccttc
      541 accggtgtcg gcgaggcgtg gacccgggac gccgacggcc ggtggtccac cgtgccggcc
      601 gtggacgcgc tcgccgcgct gggggcggag ccgttcgact ggccggtgcg cgaggcgctg
      661 gcgttcgtca acgggaccgg cgcgagcctc gcggtggctg tgctcaacca ccggtccgcc
      721 ctgcggctgg tccgcgcctg cgccgtgctc tccgcgcggc tggcgaccct gctgggggcc
      781 aatcccgagc actacgacgt ggggcacggt gtcgcgcgcg gccaggtcgg tcagctgacc
      841 gcggcggagt ggatccggca ggggctgccc cggggcatgg tgcgcgacgg cagccgcccg
      901 ctccaggagc cgtacagcct gcggtgcgcg ccgcaggtgc tcggcgcggt gctcgaccag
      961 ctcgacggcg cgggcgacgt gctggcgcgg gaggtcgacg gctgccagga caacccgatc
     1021 acctacgagg gcgagctgct gcacggcggc aacttccacg ccatgccggt gggtttcgcc
     1081 tccgaccaga tcgggttggc catgcacatg gccgcctacc tggccgagcg ccagctgggt
     1141 ctgctggtca gcccggtgac caacggcgac ctgccgccca tgctcacccc gcgcgccggg
     1201 cgcggtgccg ggctggccgg ggtgcagatc agcgcgacct cgttcgtctc gcggatccgg
     1261 cagctggtgt tccccgcctc gctgaccacc ctgccgacca acggctggaa ccaggaccac
     1321 gtgccgatgg cgctcaacgg ggcgaactcg gtgttcgagg cgttggagct cggctggctg
     1381 acggtcgggt cgctggcggt gggcgtcgcg cagctcgcgg ccatgaccgg ccacgccgcg
     1441 gagggcgtct gggcggagct ggccgggatc tgcccgccgc tggacgccga ccgcccgctg
     1501 ggcgccgagg tgcgcgccgc gcgcgacctg ctgtccgcgc acgcggacca actgctcgtc
     1561 gacgaggcag acgggaagga tttcggataa taatactaga gacggagaag cagcgaaatg
     1621 accatcacgt cacctgcgcc ggcgggccgg ctcaacaacg tccgcccgat gacgggcgag
     1681 gagtacctgg aatcactgcg ggacggccga gaggtctaca tctacggcga gcgggtcgac
     1741 gacgtcacca cgcacctggc cttccgcaac agcgtgcgct ccatcgcgcg gctctacgac
     1801 gtgctgcacg acccggcgtc cgaaggtgtg ctgcgggtgc ccaccgacac cggcaacggc
     1861 gggttcaccc acccgttctt caagaccgcc cggtcgtcgg aggacctggt cgccgcgcgc
     1921 gaggccatcg tcggctggca gcggctggtg tacgggtgga tgggccgcac cccggactac
     1981 aaggcggcgt tcttcggcac gctcgacgcc aacgccgagt tctacgggcc gttcgaggcc
     2041 aacgcccgcc gctggtaccg cgacgcccag gaacgggtgc tgtacttcaa ccacgcgatc
     2101 gtgcacccgc cggtcgaccg ggaccggccc gccgaccgga ccgccgacat ctgcgtgcac
     2161 gtggaggagg agaccgacag cgggttgatc gtctccggcg ccaaggtggt cgcgaccggc
     2221 tccgcgatga ccaacgcgaa cctcatcgcg cactacgggc ttccggtgcg ggacaagaag
     2281 ttcggcctgg tgttcacggt cccgatgaac tcgcccggcc tcaagctcat ctgccgcacc
     2341 tcctacgagc tgatggtcgc gacgcagggc tcgcccttcg actacccgct gtcgagccgg
     2401 ctcgacgaga acgactcgat catgatcttc gaccgggtgc tggtgccctg ggagaacgtg
     2461 ttcatgtacg acgcgggcgc ggccaactcc ttcgccaccg ggtcaggctt cctcgaacgc
     2521 ttcaccttcc acggctgcac ccgcctcgcg gtcaagctgg acttcatcgc cggctgcgtc
     2581 atgaaggcgg tggaggtcac cggcaccacg cacttccggg gcgtgcaggc gcaggtcggc
     2641 gaagtgctca actggcgcga cgtgttctgg ggcctgtccg acgcgatggc caagtcgccg
     2701 aactcgtggg tcggcggctc ggtgcagccg aacctcaact acgggctcgc gtaccgcacc
     2761 ttcatgggcg tgggctaccc gcgcatcaag gagatcatcc agcagaccct cggcagcggg
     2821 ttgatctacc tgaactcctc ggccgccgac tggaagaacc ccgacgtccg cccgtacctc
     2881 gaccgctacc tgcgcggctc gcggggcatc caggcgatcg accgggtcaa gctgctgaag
     2941 ctgctgtggg acgcggtcgg caccgagttc gccggccggc acgagctcta cgagcgcaac
     3001 tacggcggcg accacgaggg catccgggtg cagaccctgc aagcgtacca ggcgaacggc
     3061 caagccgccg cgctcaaggg tttcgccgag cagtgcatgt ccgagtacga cctcgacggc
     3121 tggacgaggc ccgacctgat caaccccggc acctaataat actagagctc aaggaggtac
     3181 tagatgggtt caacaggtga aactcaaata acaccaaccc acatatcaga tgaagaagca
     3241 aacctcttcg ccatgcaact agcaagtgct tcagttcttc ccatgatttt gaaatcagct
     3301 cttgaacttg atctcttaga aatcattgct aaagctggac ctggtgctca aatttcacct
     3361 attgaaattg cttctcagct accaacaact aaccctgatg caccagttat gttggaccga
     3421 atgttgcgtc tcttggcttg ttacataatc ctcacatgtt cagttcgtac tcaacaagat
     3481 ggaaaggttc agagacttta tggtttggct actgttgcta agtatttggt taagaatgaa
     3541 gatggtgtat ccatttctgc tcttaatctc atgaatcagg ataaagtgct catggaaagc
     3601 tggtaccacc taaaagatgc agtccttgat gggggcattc cattcaacaa ggcttatgga
     3661 atgacagcct ttgaatacca tggaacagat ccaaggttta acaaggtttt caacaagggg
     3721 atgtctgatc actctaccat cacaatgaag aaaattcttg agacctacac aggttttgaa
     3781 ggccttaaat ctcttgttga tgtaggtggt ggtactggag ctgtaattaa cacgattgtc
     3841 tcaaaatatc ccactataaa gggtataaat tttgatttac cccatgtcat tgaagatgct
     3901 ccatcttatc caggagttga gcatgttggt ggagacatgt ttgtcagtat tccaaaggct
     3961 gatgctgttt ttatgaagtg gatttgtcat gactggagtg atgagcactg cttgaaattt
     4021 ttgaagaact gctatgaggc actgccagac aatggaaaag tgattgtggc agaatgcata
     4081 cttccagtgg ctccagattc aagcctggcc acaaaaggtg tggttcacat tgatgtgatc
     4141 atgttggctc ataatcctgg tgggaaagag agaacacaaa aagagtttga ggatcttgcc
     4201 aaaggtgctg gattccaagg tttcaaagtc cattgtaatg ctttcaacac atacatcatg
     4261 gagtttctta agaaggttta ataa
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