some of the genes were provided by the Chris French Lab from the university of Edinburgh
this is an intermediate step in making a testing device for ferrulic acid production.
the genes sam 5 and 8 were provided by the Chris French Lab from the university of Edinburgh
this is an intermediate in making a testing device for the production of ferrulic acid.
Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
COMT (caffeic acid-O-methyl transferase) with ribosome binding site. Enzyme methylates -OH on C4 of the caffeic acid aromatic ring to produce ferulic acid. COMT is the third gene in the vanillin biosynthesis pathway,
Used COMT cDNA as genomic DNA contains introns
Alfalfa cDNA Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
COMT coding sequence Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa), synthesised by Geneart
Used cDNA as coding gene in Alfalfa genome contains introns.
strong synthetic E. coli ribosome binding site with SacI site.
This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
the genes were provided by the Chris French lab from the universisty of Edinburgh
this is the first step in making the testing device. this device test the transformation of tyrosin to ferrulic acid.
lab of Prof. C. French of the University of Edinburgh
This is an improved version of the Sam5 gene (BBa_I742105). The restriction site for PstI that was initially found at location 1436 was mutated out. The mutagenesis was done by Mr. Rashed Al Naber under supervision of Prof. C. French of the University of Edinburgh, in March 2008, using BioBrick BBa_I742105 as a template (this BioBrick was prepared by the Edinburgh iGEM 2007 team but was not submitted to the Registry).
the PstI restriction site was mutated out.
provided by the Chris French lab of the university of Edinburgh.
This is the first enzyme in the 5 step pathway to make vanillin from tyrosin. the original part was designed by Edinburgh team and can be found at number BBa_I742106
Conversion of the iGEM parts registry to SBOL2.1