LOCUS BBa_K238022 4421 bp DNA linear 14-Nov-2024 DEFINITION promoter + sam8+ sam5 + COMT + terminator ACCESSION BBa_K238022 VERSION BBa_K238022.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 RBS 44..57 /label=rbs CDS 58..1593 /label=sam8 misc_feature 44..1593 /label=BBa_K238027 RBS 1602..1617 /label=rbs CDS 1618..3159 /label=sam5 misc_feature 1602..3159 /label=BBa_K238007 misc_feature 44..3159 /label=BBa_K238019 misc_feature 1..3159 /label=BBa_K238020 misc_feature 3168..3170 /label=SacI RBS 3171..3177 /label=rbs RBS 3168..3177 /label=BBa_J15001 CDS 3184..4284 /label=COMT coding region CDS 3184..4284 /label=BBa_I742107 misc_feature 3168..4284 /label=BBa_I742109 misc_feature 1..4284 /label=BBa_K238021 stem_loop 4304..4347 /label=stem_loop misc_feature 4293..4372 /label=BBa_B0010 terminator 4293..4372 /label=BBa_B0010 stem_loop 4388..4407 /label=T7 TE misc_feature 4414..4414 /label=stop polyA_site 4408..4421 /label=polya misc_feature 4381..4421 /label=BBa_B0012 terminator 4381..4421 /label=BBa_B0012 terminator 4293..4421 /label=BBa_B0015 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gaggaggttg ggacaagatg 61 acgcaggtcg tggaacgtca ggctgatcgg ctcagcagca gggagtacct ggcccgggtc 121 gtgcgcagcg ccgggtggga cgccggtctc acctcgtgca ccgacgagga gatcgtccgg 181 atgggcgcga gcgcgcgcac catcgaggag tacctgaagt ccgacaagcc catctacggc 241 ctgacgcagg gcttcggtcc gctggtgctg ttcgacgccg actcggagct ggagcagggc 301 ggctcgctga tctcgcacct gggcaccggc cagggcgcgc cactggcccc ggaggtgtcg 361 cggctgatcc tctggctgcg catccagaac atgcgcaagg ggtactcggc ggtctcgccg 421 gtgttctggc agaagctcgc cgacctgtgg aacaaggggt tcaccccggc gatcccccgg 481 cacggcacgg tcagcgcgag cggcgacctg caaccgctgg cgcacgccgc gctcgccttc 541 accggtgtcg gcgaggcgtg gacccgggac gccgacggcc ggtggtccac cgtgccggcc 601 gtggacgcgc tcgccgcgct gggggcggag ccgttcgact ggccggtgcg cgaggcgctg 661 gcgttcgtca acgggaccgg cgcgagcctc gcggtggctg tgctcaacca ccggtccgcc 721 ctgcggctgg tccgcgcctg cgccgtgctc tccgcgcggc tggcgaccct gctgggggcc 781 aatcccgagc actacgacgt ggggcacggt gtcgcgcgcg gccaggtcgg tcagctgacc 841 gcggcggagt ggatccggca ggggctgccc cggggcatgg tgcgcgacgg cagccgcccg 901 ctccaggagc cgtacagcct gcggtgcgcg ccgcaggtgc tcggcgcggt gctcgaccag 961 ctcgacggcg cgggcgacgt gctggcgcgg gaggtcgacg gctgccagga caacccgatc 1021 acctacgagg gcgagctgct gcacggcggc aacttccacg ccatgccggt gggtttcgcc 1081 tccgaccaga tcgggttggc catgcacatg gccgcctacc tggccgagcg ccagctgggt 1141 ctgctggtca gcccggtgac caacggcgac ctgccgccca tgctcacccc gcgcgccggg 1201 cgcggtgccg ggctggccgg ggtgcagatc agcgcgacct cgttcgtctc gcggatccgg 1261 cagctggtgt tccccgcctc gctgaccacc ctgccgacca acggctggaa ccaggaccac 1321 gtgccgatgg cgctcaacgg ggcgaactcg gtgttcgagg cgttggagct cggctggctg 1381 acggtcgggt cgctggcggt gggcgtcgcg cagctcgcgg ccatgaccgg ccacgccgcg 1441 gagggcgtct gggcggagct ggccgggatc tgcccgccgc tggacgccga ccgcccgctg 1501 ggcgccgagg tgcgcgccgc gcgcgacctg ctgtccgcgc acgcggacca actgctcgtc 1561 gacgaggcag acgggaagga tttcggataa taatactaga gacggagaag cagcgaaatg 1621 accatcacgt cacctgcgcc ggcgggccgg ctcaacaacg tccgcccgat gacgggcgag 1681 gagtacctgg aatcactgcg ggacggccga gaggtctaca tctacggcga gcgggtcgac 1741 gacgtcacca cgcacctggc cttccgcaac agcgtgcgct ccatcgcgcg gctctacgac 1801 gtgctgcacg acccggcgtc cgaaggtgtg ctgcgggtgc ccaccgacac cggcaacggc 1861 gggttcaccc acccgttctt caagaccgcc cggtcgtcgg aggacctggt cgccgcgcgc 1921 gaggccatcg tcggctggca gcggctggtg tacgggtgga tgggccgcac cccggactac 1981 aaggcggcgt tcttcggcac gctcgacgcc aacgccgagt tctacgggcc gttcgaggcc 2041 aacgcccgcc gctggtaccg cgacgcccag gaacgggtgc tgtacttcaa ccacgcgatc 2101 gtgcacccgc cggtcgaccg ggaccggccc gccgaccgga ccgccgacat ctgcgtgcac 2161 gtggaggagg agaccgacag cgggttgatc gtctccggcg ccaaggtggt cgcgaccggc 2221 tccgcgatga ccaacgcgaa cctcatcgcg cactacgggc ttccggtgcg ggacaagaag 2281 ttcggcctgg tgttcacggt cccgatgaac tcgcccggcc tcaagctcat ctgccgcacc 2341 tcctacgagc tgatggtcgc gacgcagggc tcgcccttcg actacccgct gtcgagccgg 2401 ctcgacgaga acgactcgat catgatcttc gaccgggtgc tggtgccctg ggagaacgtg 2461 ttcatgtacg acgcgggcgc ggccaactcc ttcgccaccg ggtcaggctt cctcgaacgc 2521 ttcaccttcc acggctgcac ccgcctcgcg gtcaagctgg acttcatcgc cggctgcgtc 2581 atgaaggcgg tggaggtcac cggcaccacg cacttccggg gcgtgcaggc gcaggtcggc 2641 gaagtgctca actggcgcga cgtgttctgg ggcctgtccg acgcgatggc caagtcgccg 2701 aactcgtggg tcggcggctc ggtgcagccg aacctcaact acgggctcgc gtaccgcacc 2761 ttcatgggcg tgggctaccc gcgcatcaag gagatcatcc agcagaccct cggcagcggg 2821 ttgatctacc tgaactcctc ggccgccgac tggaagaacc ccgacgtccg cccgtacctc 2881 gaccgctacc tgcgcggctc gcggggcatc caggcgatcg accgggtcaa gctgctgaag 2941 ctgctgtggg acgcggtcgg caccgagttc gccggccggc acgagctcta cgagcgcaac 3001 tacggcggcg accacgaggg catccgggtg cagaccctgc aagcgtacca ggcgaacggc 3061 caagccgccg cgctcaaggg tttcgccgag cagtgcatgt ccgagtacga cctcgacggc 3121 tggacgaggc ccgacctgat caaccccggc acctaataat actagagctc aaggaggtac 3181 tagatgggtt caacaggtga aactcaaata acaccaaccc acatatcaga tgaagaagca 3241 aacctcttcg ccatgcaact agcaagtgct tcagttcttc ccatgatttt gaaatcagct 3301 cttgaacttg atctcttaga aatcattgct aaagctggac ctggtgctca aatttcacct 3361 attgaaattg cttctcagct accaacaact aaccctgatg caccagttat gttggaccga 3421 atgttgcgtc tcttggcttg ttacataatc ctcacatgtt cagttcgtac tcaacaagat 3481 ggaaaggttc agagacttta tggtttggct actgttgcta agtatttggt taagaatgaa 3541 gatggtgtat ccatttctgc tcttaatctc atgaatcagg ataaagtgct catggaaagc 3601 tggtaccacc taaaagatgc agtccttgat gggggcattc cattcaacaa ggcttatgga 3661 atgacagcct ttgaatacca tggaacagat ccaaggttta acaaggtttt caacaagggg 3721 atgtctgatc actctaccat cacaatgaag aaaattcttg agacctacac aggttttgaa 3781 ggccttaaat ctcttgttga tgtaggtggt ggtactggag ctgtaattaa cacgattgtc 3841 tcaaaatatc ccactataaa gggtataaat tttgatttac cccatgtcat tgaagatgct 3901 ccatcttatc caggagttga gcatgttggt ggagacatgt ttgtcagtat tccaaaggct 3961 gatgctgttt ttatgaagtg gatttgtcat gactggagtg atgagcactg cttgaaattt 4021 ttgaagaact gctatgaggc actgccagac aatggaaaag tgattgtggc agaatgcata 4081 cttccagtgg ctccagattc aagcctggcc acaaaaggtg tggttcacat tgatgtgatc 4141 atgttggctc ataatcctgg tgggaaagag agaacacaaa aagagtttga ggatcttgcc 4201 aaaggtgctg gattccaagg tttcaaagtc cattgtaatg ctttcaacac atacatcatg 4261 gagtttctta agaaggttta ataatactag agccaggcat caaataaaac gaaaggctca 4321 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 4381 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //