BBa_K239003
1
BBa_K239003
Modified HypB promoter, activated by Fnr during oxygen deficiency
2009-06-21T11:00:00Z
2015-05-08T01:11:36Z
Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.
Sequence contains 2 Fnr binding sites but no specific promoter sequence. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism.
false
false
_375_
0
4247
9
Not in stock
false
Optimisation of Fnr binding sites, modification of position 2 bp.
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed.
Sequence starts 3 bp before the first Fnr binding site and ends 35 bp after second Fnr binding site.
false
Axel Nystrom
annotation2006684
1
Fnr
range2006684
1
110
123
annotation2006683
1
Fnr
range2006683
1
4
17
BBa_K239003_sequence
1
gaattgatcgaaatcaaggccgcaaaacacggcgcaaaacgcgtaactggggtctggctcaaaattggcgcattttcttgtgtcgaaaccagctctcttgccttttgttttgatctggatcaacggcagcgtggcggaaggttgtaaactgcacctcg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z