LOCUS       BBa_K239009             1045 bp    DNA     linear       05-Nov-2024
DEFINITION  GFP test devise for Spy promoter
ACCESSION   BBa_K239009
VERSION     BBa_K239009.1  
FEATURES             Location/Qualifiers
     promoter        150..157
                     /label=-10
     promoter        128..136
                     /label=-35
     misc_feature    4..22
                     /label=BaeR
     misc_feature    66..81
                     /label=BaeR
     misc_feature    107..120
                     /label=CpxR
     promoter        1..162
                     /label=BBa_K239001
     misc_feature    175..178
                     /label=conserved
     RBS             171..182
                     /label=BBa_B0034
     CDS             189..908
                     /label=GFP protein
     CDS             189..908
                     /label=BBa_E0040
     stem_loop       928..971
                     /label=stem_loop
     misc_feature    917..996
                     /label=BBa_B0010
     terminator      917..996
                     /label=BBa_B0010
     stem_loop       1012..1031
                     /label=T7 TE
     misc_feature    1038..1038
                     /label=stop
     polyA_site      1032..1045
                     /label=polya
     misc_feature    1005..1045
                     /label=BBa_B0012
     terminator      1005..1045
                     /label=BBa_B0012
ORIGIN
        1 agttcttcat aatctctgca aaatcatcgt gttgtaatat tctctcatca ctctccatca
       61 aattttcttt ttttctccat aattggcgca aaagtgtttt ttacactttc attgttttac
      121 cgttgctctg attaattgac gctaaagtca gtaaagttaa tctactagag aaagaggaga
      181 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      241 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      301 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      361 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
      421 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      481 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
      541 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
      601 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
      661 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
      721 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
      781 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
      841 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
      901 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      961 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1021 tcgggtgggc ctttctgcgt ttata
//