LOCUS       BBa_K239015             1116 bp    DNA     linear       22-Nov-2024
DEFINITION  GFP test devise for DegP promoter (BBa_K239000)
ACCESSION   BBa_K239015
VERSION     BBa_K239015.1  
FEATURES             Location/Qualifiers
     misc_feature    4..19
                     /label=CpxR
     misc_feature    154..171
                     /label=CpxR
     misc_feature    164..178
                     /label=CpxR
     promoter        201..206
                     /label=-35 box
     promoter        223..227
                     /label=-10 box
     promoter        1..233
                     /label=BBa_K239000
     misc_feature    246..249
                     /label=conserved
     RBS             242..253
                     /label=BBa_B0034
     CDS             260..979
                     /label=GFP protein
     CDS             260..979
                     /label=BBa_E0040
     stem_loop       999..1042
                     /label=stem_loop
     misc_feature    988..1067
                     /label=BBa_B0010
     terminator      988..1067
                     /label=BBa_B0010
     stem_loop       1083..1102
                     /label=T7 TE
     misc_feature    1109..1109
                     /label=stop
     polyA_site      1103..1116
                     /label=polya
     misc_feature    1076..1116
                     /label=BBa_B0012
     terminator      1076..1116
                     /label=BBa_B0012
ORIGIN
        1 tcagtaaatt accgtcagat tctcctgagt ttccgctatg ggaatattat taccgttgcc
       61 gcctgctgga ggattatatc agcggtatga ccgacctcta tgcgtgggat gaataccgac
      121 gtctgatggc cgtagaacaa taaccaggct tttgtaaaga cgaacaataa atttttacct
      181 tttgcagaaa ctttagttcg gaacttcagg ctataaaacg aatctgaaga acatactaga
      241 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      301 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      361 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      421 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      481 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      541 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      601 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      661 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      721 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      781 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      841 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      901 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      961 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1021 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1081 ctggctcacc ttcgggtggg cctttctgcg tttata
//