LOCUS       BBa_K241000             2841 bp    DNA     linear       22-Dec-2024
DEFINITION  Nuclease construct, no sense promoter.
ACCESSION   BBa_K241000
VERSION     BBa_K241000.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..921
                     /label=BBa_K093001
     misc_feature    934..937
                     /label=conserved
     RBS             930..941
                     /label=BBa_B0034
     CDS             948..1640
                     /label=BBa_K093002
     misc_feature    930..1640
                     /label=BBa_K093013
     promoter        1656..1662
                     /label=-10
     promoter        1678..1683
                     /label=-35
     misc_feature    1684..1700
                     /label=lacO1
     misc_feature    1663..1677
                     /label=lacO1
     promoter        1649..1703
                     /label=BBa_K093008
     stem_loop       1723..1766
                     /label=stem_loop
     misc_feature    1712..1791
                     /label=BBa_B0010
     terminator      1712..1791
                     /label=BBa_B0010
     stem_loop       1807..1826
                     /label=T7 TE
     misc_feature    1833..1833
                     /label=stop
     polyA_site      1827..1840
                     /label=polya
     misc_feature    1800..1840
                     /label=BBa_B0012
     terminator      1800..1840
                     /label=BBa_B0012
     misc_feature    1649..1840
                     /label=BBa_K093004
     misc_feature    1851..1867
                     /label=lac O1
     promoter        1868..1873
                     /label=-35
     misc_feature    1874..1890
                     /label=lac O1
     promoter        1891..1896
                     /label=-10
     misc_feature    1849..1902
                     /label=BBa_R0011
     promoter        1849..1902
                     /label=BBa_R0011
     misc_feature    1916..1919
                     /label=conserved
     RBS             1912..1923
                     /label=BBa_B0034
     CDS             1933..2640
                     /label=cI lambda
     misc_feature    2641..2673
                     /label=LVA
     misc_feature    2680..2704
                     /label=Help:Barcodes
     CDS             1930..2704
                     /label=BBa_C0051
     misc_feature    1912..2704
                     /label=BBa_K093006
     stem_loop       2724..2767
                     /label=stem_loop
     misc_feature    2713..2792
                     /label=BBa_B0010
     terminator      2713..2792
                     /label=BBa_B0010
     stem_loop       2808..2827
                     /label=T7 TE
     misc_feature    2834..2834
                     /label=stop
     polyA_site      2828..2841
                     /label=polya
     misc_feature    2801..2841
                     /label=BBa_B0012
     terminator      2801..2841
                     /label=BBa_B0012
     terminator      2713..2841
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat ggcacttctt gaccttaaac aattctatga gttacgtgaa
       61 ggctgcgacg acaagggtat ccttgtgatg gacggcgact ggctggtctt ccaagctatg
      121 agtgctgctg agtttgatgc ctcttgggag gaagagattt ggcaccgatg ctgtgaccac
      181 gctaaggccc gtcagattct tgaggattcc attaagtcct acgagacccg taagaaggct
      241 tgggcaggtg ctccaattgt ccttgcgttc accgatagtg ttaactggcg taaagaactg
      301 gttgacccga actataaggc taaccgtaag gccgtgaaga aacctgtagg gtactttgag
      361 ttccttgatg ctctctttga gcgcgaagag ttctattgca tccgtgagcc tatgcttgag
      421 ggtgatgacg ttatgggagt tattgcttcc aatccgtctg ccttcggtgc tcgtaaggct
      481 gtaatcatct cttgcgataa ggactttaag accatcccta actgtgactt cctgtggtgt
      541 accactggta acatcctgac tcagaccgaa gagtccgctg actggtggca cctcttccag
      601 accatcaagg gtgacatcac tgatggttac tcagggattg ctggatgggg tgataccgcc
      661 gaggacttct tgaataaccc gttcataacc gagcctaaaa cgtctgtgct taagtccggt
      721 aagaacaaag gccaagaggt tactaaatgg gttaaacgcg accctgagcc tcatgagacg
      781 ctttgggact gcattaagtc cattggcgcg aaggctggta tgaccgaaga ggatattatc
      841 aagcagggcc aaatggctcg aatcctacgg ttcaacgagt acaactttat tgacaaggag
      901 atttacctgt ggagaccgta atactagaga aagaggagaa atactagatg acaacaaact
      961 ccccctcaga cgtcggcatg atcgacgagt gtctgtccat cgtccgaacg tcgcttgcac
     1021 gatgtttcca acagcaggcc ccaagcattc aagcctcatg gccactttca ggacgcgccg
     1081 tatctgagat tggaggccgc ctagtcgaga gtttcgtttt agcacgactc ccgcatgaac
     1141 tgagcaccac gccttttgac ggccagattc tatgtgaaat acctgaatcc ggcagagcga
     1201 tggaagacat tgcggtgacc ttcatcggcc cacatggaag ggctcgacta ctcatcgacg
     1261 tcaagggtca taacgaatac cgcacgggat cgagacccaa tttggcttcg atccgaaaat
     1321 gtctggaact ctatcgcagc tcctcacata ccgttgatga gctcgttgtc ttcttctgcc
     1381 gttaccgccc atccgtccac ccggatcatc acgcacaagc ggtcgaatat cacgttctgc
     1441 ccgagtcgtt aatgagcagg gattttcctg cttcgtgccc tgagcgaaag caacctggat
     1501 ccagccaata tcggagtggc ggccagttgc tgcttgccag ggaaaacaac atacggttag
     1561 tgaatcgttc aaggtcggag ttcgttcaac ttctggaggg tctccagtca cgccttcaac
     1621 gggggcgaag tacggtttaa tactagagtg tgctcagtat cttgttatcc gctcacaatg
     1681 tcaattgtta tccgctcaca atttactaga gccaggcatc aaataaaacg aaaggctcag
     1741 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1801 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa ttgtgagcgg
     1861 ataacaattg acattgtgag cggataacaa gatactgagc acatactaga gaaagaggag
     1921 aaatactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt
     1981 cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc
     2041 gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca
     2101 ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt
     2161 agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca
     2221 cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct
     2281 gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc
     2341 agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc
     2401 aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca
     2461 ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg
     2521 gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat
     2581 gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc
     2641 gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga
     2701 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2761 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2821 gtgggccttt ctgcgtttat a
//