BBa_K241000
type: ComponentDefinition
persistent identity: BBa K241000
display id: BBa_K241000
version: 1
was generated by: iGEM to SBOL conversion
description: Nuclease construct, no sense promoter.
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:37Z
mutable provenance: Synthetic, Phage T7, Phage Lambda, Pseudomonas mendocina.
owned by: james
owned by: myers
mutable description: To tightly repress the nuclease genes (T7 gene 6, PmeI) an antisense promoter and the cI repressor are used. The promoter for expression of the integrase plasmid (the sense promoter) is not on this part. However, the design calls for the Lambda Pr promoter. The part is designed to selectively degrade DNA in vivo.
discontinued: false
dominant: false
group u list: _334_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The main design consideration was to have maximal repression of the toxic nuclease genes.
star: false
creator: John Heil
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K093013
component: BBa_K093001
component: BBa_R0011
component: BBa_K093004
component: BBa_B0015
component: BBa_K093006
sequence annotation: BBa_B0034
sequence annotation: BBa_K093001
sequence annotation: BBa_K093013
sequence annotation: BBa_K093004
sequence annotation: BBa_R0011
sequence annotation: BBa_K093006
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K241000_sequence/1

BBa_B0034
type: Component
persistent identity: component2297579
display id: component2297579
version: 1
top level: BBa_K241000
definition: BBa_B0034
access: public

BBa_K093013
type: Component
persistent identity: component2297584
display id: component2297584
version: 1
top level: BBa_K241000
definition: RBS-PmeIR
access: public

BBa_K093001
type: Component
persistent identity: component2297580
display id: component2297580
version: 1
top level: BBa_K241000
definition: T7Gene6
access: public

BBa_R0011
type: Component
persistent identity: component2297597
display id: component2297597
version: 1
top level: BBa_K241000
definition: lacI+pL
access: public

BBa_K093004
type: Component
persistent identity: component2297596
display id: component2297596
version: 1
top level: BBa_K241000
definition: rPlac+TT
access: public

BBa_B0015
type: Component
persistent identity: component2297615
display id: component2297615
version: 1
top level: BBa_K241000
definition: BBa_B0015
access: public

BBa_K093006
type: Component
persistent identity: component2297608
display id: component2297608
version: 1
top level: BBa_K241000
definition: RBS-cI
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2297579
display id: annotation2297579
version: 1
top level: BBa_K241000
location: https://synbiohub.org/public/igem/BBa_K241000/annotation2297579/range2297579/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K241000/annotation2297579/range2297579/1
type: Range
persistent identity: range2297579
display id: range2297579
version: 1
top level: BBa_K241000
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K093001
type: SequenceAnnotation
persistent identity: annotation2297580
display id: annotation2297580
version: 1
top level: BBa_K241000
location: https://synbiohub.org/public/igem/BBa_K241000/annotation2297580/range2297580/1
component: BBa_K093001

https://synbiohub.org/public/igem/BBa_K241000/annotation2297580/range2297580/1
type: Range
persistent identity: range2297580
display id: range2297580
version: 1
top level: BBa_K241000
direction: direction/reverse
start: 19
end: 921
orientation: inline

BBa_K093013
type: SequenceAnnotation
persistent identity: annotation2297584
display id: annotation2297584
version: 1
top level: BBa_K241000
location: https://synbiohub.org/public/igem/BBa_K241000/annotation2297584/range2297584/1
component: BBa_K093013

https://synbiohub.org/public/igem/BBa_K241000/annotation2297584/range2297584/1
type: Range
persistent identity: range2297584
display id: range2297584
version: 1
top level: BBa_K241000
direction: direction/reverse
start: 930
end: 1640
orientation: inline

BBa_K093004
type: SequenceAnnotation
persistent identity: annotation2297596
display id: annotation2297596
version: 1
top level: BBa_K241000
location: https://synbiohub.org/public/igem/BBa_K241000/annotation2297596/range2297596/1
component: BBa_K093004

https://synbiohub.org/public/igem/BBa_K241000/annotation2297596/range2297596/1
type: Range
persistent identity: range2297596
display id: range2297596
version: 1
top level: BBa_K241000
direction: direction/reverse
start: 1649
end: 1840
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2297597
display id: annotation2297597
version: 1
top level: BBa_K241000
location: https://synbiohub.org/public/igem/BBa_K241000/annotation2297597/range2297597/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K241000/annotation2297597/range2297597/1
type: Range
persistent identity: range2297597
display id: range2297597
version: 1
top level: BBa_K241000
direction: direction/forward
start: 1849
end: 1902
orientation: inline

BBa_K093006
type: SequenceAnnotation
persistent identity: annotation2297608
display id: annotation2297608
version: 1
top level: BBa_K241000
location: https://synbiohub.org/public/igem/BBa_K241000/annotation2297608/range2297608/1
component: BBa_K093006

https://synbiohub.org/public/igem/BBa_K241000/annotation2297608/range2297608/1
type: Range
persistent identity: range2297608
display id: range2297608
version: 1
top level: BBa_K241000
direction: direction/reverse
start: 1912
end: 2704
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2297615
display id: annotation2297615
version: 1
top level: BBa_K241000
location: https://synbiohub.org/public/igem/BBa_K241000/annotation2297615/range2297615/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K241000/annotation2297615/range2297615/1
type: Range
persistent identity: range2297615
display id: range2297615
version: 1
top level: BBa_K241000
direction: direction/reverse
start: 2713
end: 2841
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

T7Gene6
type: ComponentDefinition
persistent identity: BBa K093001
display id: BBa_K093001
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Gene 6 exonuclease
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: T7 genomic DNA
owned by: james
owned by: myers
mutable description: This is the T7 gene 6 ORF, that codes for the T7 gene 6 5'-3' exonuclease. For properties of T7 gene 6 exonuclease see: Kerr and Sadowski "Gene 6 Exonuclease of Bacteriophage T7" The Journal of Biological Chemistry Vol. 247 No. 1 January 10, pp 311-318, 1972.
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We needed to change the stop codon to the standard stop codon TAA. We later realized that we should have added tandem TAA. The Tandem TAA stop codon is present in the composite parts containing this ORF. The ORF alone has the single TAA stop codon.
star: false
creator: John Heil, Shira Davis, Danielle Nash
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K093001_sequence/1

rPlac+TT
type: ComponentDefinition
persistent identity: BBa K093004
display id: BBa_K093004
version: 1
was generated by: iGEM to SBOL conversion
description: Convergent Promoter System: Reverse Module: rPlac+TT
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part contains a reverse BBa_R0011 followed by a forward TT, BBa_B0015.
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The forward and reverse efficiencies of TTs in the registry had to be considered, especially for this part's convergent partner, BBa_K093003. R0011 was used instead of R0010 to avoid catabolite repression. The idea is to place BBa_K093003 upstream of the gene of interest, that requires tight repression, and to place BBa_K093004 downstream. If the cells are grown in the presence of IPTG POlacI is induced and anti-sense mRNA is produced. Anti-sense mRNA will repress expression of the gene of interest through RNAi. This system also requires cI repressor (BBa_C0051) under control of POlacI (BBa_R0011). This system would be functional in a laqIq strain such as E. coli. TG1. O'Connor,C.D., & Timmins, K.N. (1987). Highly repressible expression system for cloning genes that specify potentialy toxic proteins. Journal of Bacteriology. 169, 4457-4462.
star: false
creator: John Heil, Danielle Nash, Shira Davis
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0010
component: BBa_K093008
sequence annotation: BBa_K093008
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K093004_sequence/1

BBa_B0012
type: Component
persistent identity: component1996703
display id: component1996703
version: 1
top level: rPlac+TT
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1996701
display id: component1996701
version: 1
top level: rPlac+TT
definition: BBa_B0010
access: public

BBa_K093008
type: Component
persistent identity: component1996700
display id: component1996700
version: 1
top level: rPlac+TT
definition: BBa_K093008
access: public

BBa_K093008
type: SequenceAnnotation
persistent identity: annotation1996700
display id: annotation1996700
version: 1
top level: rPlac+TT
location: https://synbiohub.org/public/igem/BBa_K093004/annotation1996700/range1996700/1
component: BBa_K093008

https://synbiohub.org/public/igem/BBa_K093004/annotation1996700/range1996700/1
type: Range
persistent identity: range1996700
display id: range1996700
version: 1
top level: rPlac+TT
direction: direction/reverse
start: 1
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1996701
display id: annotation1996701
version: 1
top level: rPlac+TT
location: https://synbiohub.org/public/igem/BBa_K093004/annotation1996701/range1996701/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K093004/annotation1996701/range1996701/1
type: Range
persistent identity: range1996701
display id: range1996701
version: 1
top level: rPlac+TT
direction: direction/forward
start: 64
end: 143
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1996703
display id: annotation1996703
version: 1
top level: rPlac+TT
location: https://synbiohub.org/public/igem/BBa_K093004/annotation1996703/range1996703/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K093004/annotation1996703/range1996703/1
type: Range
persistent identity: range1996703
display id: range1996703
version: 1
top level: rPlac+TT
direction: direction/forward
start: 152
end: 192
orientation: inline

RBS-cI
type: ComponentDefinition
persistent identity: BBa K093006
display id: BBa_K093006
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor with RBS
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: registry plasmids
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: cI repressor, BBa_C0051, with Elowitz RBS, BBa_B0034.
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Needed for downstream applications.
star: false
creator: John Heil
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_K093006_sequence/1

BBa_B0034
type: Component
persistent identity: component2244027
display id: component2244027
version: 1
top level: RBS-cI
definition: BBa_B0034
access: public

BBa_C0051
type: Component
persistent identity: component2244031
display id: component2244031
version: 1
top level: RBS-cI
definition: cI lam
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244027
display id: annotation2244027
version: 1
top level: RBS-cI
location: https://synbiohub.org/public/igem/BBa_K093006/annotation2244027/range2244027/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K093006/annotation2244027/range2244027/1
type: Range
persistent identity: range2244027
display id: range2244027
version: 1
top level: RBS-cI
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2244031
display id: annotation2244031
version: 1
top level: RBS-cI
location: https://synbiohub.org/public/igem/BBa_K093006/annotation2244031/range2244031/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K093006/annotation2244031/range2244031/1
type: Range
persistent identity: range2244031
display id: range2244031
version: 1
top level: RBS-cI
direction: direction/reverse
start: 19
end: 793
orientation: inline

RBS-PmeIR
type: ComponentDefinition
persistent identity: BBa K093013
display id: BBa_K093013
version: 1
was generated by: iGEM to SBOL conversion
description: PmeI endonuclease with RBS
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: composite part
owned by: james
owned by: myers
mutable description: PmeI with RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We wanted to express PmeI. That requires a RBS.
star: false
creator: John Heil, Danielle Nash
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_K093002
sequence annotation: BBa_B0034
sequence annotation: BBa_K093002
sequence: https://synbiohub.org/public/igem/BBa_K093013_sequence/1

BBa_B0034
type: Component
persistent identity: component1996874
display id: component1996874
version: 1
top level: RBS-PmeIR
definition: BBa_B0034
access: public

BBa_K093002
type: Component
persistent identity: component1996875
display id: component1996875
version: 1
top level: RBS-PmeIR
definition: PmeIR
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1996874
display id: annotation1996874
version: 1
top level: RBS-PmeIR
location: https://synbiohub.org/public/igem/BBa_K093013/annotation1996874/range1996874/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K093013/annotation1996874/range1996874/1
type: Range
persistent identity: range1996874
display id: range1996874
version: 1
top level: RBS-PmeIR
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K093002
type: SequenceAnnotation
persistent identity: annotation1996875
display id: annotation1996875
version: 1
top level: RBS-PmeIR
location: https://synbiohub.org/public/igem/BBa_K093013/annotation1996875/range1996875/1
component: BBa_K093002

https://synbiohub.org/public/igem/BBa_K093013/annotation1996875/range1996875/1
type: Range
persistent identity: range1996875
display id: range1996875
version: 1
top level: RBS-PmeIR
direction: direction/reverse
start: 19
end: 711
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

PmeIR
type: ComponentDefinition
persistent identity: BBa K093002
display id: BBa_K093002
version: 1
was generated by: iGEM to SBOL conversion
description: PmeI endonuclease
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: Synthetic from GENEART
owned by: james
owned by: myers
mutable description: This is the PmeI ORF, coding for the PmeI restriction endonuclease gene with the recognition sequence: GTTTAAAC
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The stop codon was changed to TAA.
star: false
creator: John Heil
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K093002_sequence/1

BBa_K093008
type: ComponentDefinition
persistent identity: BBa K093008
display id: BBa_K093008
version: 1
was generated by: iGEM to SBOL conversion
description: reverse BBa_R0011
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: reverse compliments of BBa_R0011
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: for downstream application
star: false
creator: John Heil
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: -10
sequence annotation: lacO1
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K093008_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1992540
display id: annotation1992540
version: 1
top level: BBa_K093008
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992540/range1992540/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K093008/annotation1992540/range1992540/1
type: Range
persistent identity: range1992540
display id: range1992540
version: 1
top level: BBa_K093008
direction: direction/reverse
start: 8
end: 14
orientation: inline

lacO1
type: SequenceAnnotation
persistent identity: annotation1992543
display id: annotation1992543
version: 1
top level: BBa_K093008
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992543/range1992543/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K093008/annotation1992543/range1992543/1
type: Range
persistent identity: range1992543
display id: range1992543
version: 1
top level: BBa_K093008
direction: direction/reverse
start: 15
end: 29
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1992541
display id: annotation1992541
version: 1
top level: BBa_K093008
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992541/range1992541/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K093008/annotation1992541/range1992541/1
type: Range
persistent identity: range1992541
display id: range1992541
version: 1
top level: BBa_K093008
direction: direction/reverse
start: 30
end: 35
orientation: inline

lacO1
type: SequenceAnnotation
persistent identity: annotation1992542
display id: annotation1992542
version: 1
top level: BBa_K093008
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992542/range1992542/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K093008/annotation1992542/range1992542/1
type: Range
persistent identity: range1992542
display id: range1992542
version: 1
top level: BBa_K093008
direction: direction/reverse
start: 36
end: 52
orientation: inline

https://synbiohub.org/public/igem/BBa_K241000_sequence/1
type: Sequence
persistent identity: BBa K241000 sequence
display id: BBa_K241000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcacttcttgaccttaaacaattctatgagttacgtgaaggctgcgacgacaagggtatccttgtgatggacggcgactggctggtcttccaagctatgagtgctgctgagtttgatgcctcttgggaggaagagatttggcaccgatgctgtgaccacgctaaggcccgtcagattcttgaggattccattaagtcctacgagacccgtaagaaggcttgggcaggtgctccaattgtccttgcgttcaccgatagtgttaactggcgtaaagaactggttgacccgaactataaggctaaccgtaaggccgtgaagaaacctgtagggtactttgagttccttgatgctctctttgagcgcgaagagttctattgcatccgtgagcctatgcttgagggtgatgacgttatgggagttattgcttccaatccgtctgccttcggtgctcgtaaggctgtaatcatctcttgcgataaggactttaagaccatccctaactgtgacttcctgtggtgtaccactggtaacatcctgactcagaccgaagagtccgctgactggtggcacctcttccagaccatcaagggtgacatcactgatggttactcagggattgctggatggggtgataccgccgaggacttcttgaataacccgttcataaccgagcctaaaacgtctgtgcttaagtccggtaagaacaaaggccaagaggttactaaatgggttaaacgcgaccctgagcctcatgagacgctttgggactgcattaagtccattggcgcgaaggctggtatgaccgaagaggatattatcaagcagggccaaatggctcgaatcctacggttcaacgagtacaactttattgacaaggagatttacctgtggagaccgtaatactagagaaagaggagaaatactagatgacaacaaactccccctcagacgtcggcatgatcgacgagtgtctgtccatcgtccgaacgtcgcttgcacgatgtttccaacagcaggccccaagcattcaagcctcatggccactttcaggacgcgccgtatctgagattggaggccgcctagtcgagagtttcgttttagcacgactcccgcatgaactgagcaccacgccttttgacggccagattctatgtgaaatacctgaatccggcagagcgatggaagacattgcggtgaccttcatcggcccacatggaagggctcgactactcatcgacgtcaagggtcataacgaataccgcacgggatcgagacccaatttggcttcgatccgaaaatgtctggaactctatcgcagctcctcacataccgttgatgagctcgttgtcttcttctgccgttaccgcccatccgtccacccggatcatcacgcacaagcggtcgaatatcacgttctgcccgagtcgttaatgagcagggattttcctgcttcgtgccctgagcgaaagcaacctggatccagccaatatcggagtggcggccagttgctgcttgccagggaaaacaacatacggttagtgaatcgttcaaggtcggagttcgttcaacttctggagggtctccagtcacgccttcaacgggggcgaagtacggtttaatactagagtgtgctcagtatcttgttatccgctcacaatgtcaattgttatccgctcacaatttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093001_sequence/1
type: Sequence
persistent identity: BBa K093001 sequence
display id: BBa_K093001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcacttcttgaccttaaacaattctatgagttacgtgaaggctgcgacgacaagggtatccttgtgatggacggcgactggctggtcttccaagctatgagtgctgctgagtttgatgcctcttgggaggaagagatttggcaccgatgctgtgaccacgctaaggcccgtcagattcttgaggattccattaagtcctacgagacccgtaagaaggcttgggcaggtgctccaattgtccttgcgttcaccgatagtgttaactggcgtaaagaactggttgacccgaactataaggctaaccgtaaggccgtgaagaaacctgtagggtactttgagttccttgatgctctctttgagcgcgaagagttctattgcatccgtgagcctatgcttgagggtgatgacgttatgggagttattgcttccaatccgtctgccttcggtgctcgtaaggctgtaatcatctcttgcgataaggactttaagaccatccctaactgtgacttcctgtggtgtaccactggtaacatcctgactcagaccgaagagtccgctgactggtggcacctcttccagaccatcaagggtgacatcactgatggttactcagggattgctggatggggtgataccgccgaggacttcttgaataacccgttcataaccgagcctaaaacgtctgtgcttaagtccggtaagaacaaaggccaagaggttactaaatgggttaaacgcgaccctgagcctcatgagacgctttgggactgcattaagtccattggcgcgaaggctggtatgaccgaagaggatattatcaagcagggccaaatggctcgaatcctacggttcaacgagtacaactttattgacaaggagatttacctgtggagaccgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093004_sequence/1
type: Sequence
persistent identity: BBa K093004 sequence
display id: BBa_K093004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtgctcagtatcttgttatccgctcacaatgtcaattgttatccgctcacaatttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093006_sequence/1
type: Sequence
persistent identity: BBa K093006 sequence
display id: BBa_K093006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093013_sequence/1
type: Sequence
persistent identity: BBa K093013 sequence
display id: BBa_K093013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgacaacaaactccccctcagacgtcggcatgatcgacgagtgtctgtccatcgtccgaacgtcgcttgcacgatgtttccaacagcaggccccaagcattcaagcctcatggccactttcaggacgcgccgtatctgagattggaggccgcctagtcgagagtttcgttttagcacgactcccgcatgaactgagcaccacgccttttgacggccagattctatgtgaaatacctgaatccggcagagcgatggaagacattgcggtgaccttcatcggcccacatggaagggctcgactactcatcgacgtcaagggtcataacgaataccgcacgggatcgagacccaatttggcttcgatccgaaaatgtctggaactctatcgcagctcctcacataccgttgatgagctcgttgtcttcttctgccgttaccgcccatccgtccacccggatcatcacgcacaagcggtcgaatatcacgttctgcccgagtcgttaatgagcagggattttcctgcttcgtgccctgagcgaaagcaacctggatccagccaatatcggagtggcggccagttgctgcttgccagggaaaacaacatacggttagtgaatcgttcaaggtcggagttcgttcaacttctggagggtctccagtcacgccttcaacgggggcgaagtacggtttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093002_sequence/1
type: Sequence
persistent identity: BBa K093002 sequence
display id: BBa_K093002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacaacaaactccccctcagacgtcggcatgatcgacgagtgtctgtccatcgtccgaacgtcgcttgcacgatgtttccaacagcaggccccaagcattcaagcctcatggccactttcaggacgcgccgtatctgagattggaggccgcctagtcgagagtttcgttttagcacgactcccgcatgaactgagcaccacgccttttgacggccagattctatgtgaaatacctgaatccggcagagcgatggaagacattgcggtgaccttcatcggcccacatggaagggctcgactactcatcgacgtcaagggtcataacgaataccgcacgggatcgagacccaatttggcttcgatccgaaaatgtctggaactctatcgcagctcctcacataccgttgatgagctcgttgtcttcttctgccgttaccgcccatccgtccacccggatcatcacgcacaagcggtcgaatatcacgttctgcccgagtcgttaatgagcagggattttcctgcttcgtgccctgagcgaaagcaacctggatccagccaatatcggagtggcggccagttgctgcttgccagggaaaacaacatacggttagtgaatcgttcaaggtcggagttcgttcaacttctggagggtctccagtcacgccttcaacgggggcgaagtacggtttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093008_sequence/1
type: Sequence
persistent identity: BBa K093008 sequence
display id: BBa_K093008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtgctcagtatcttgttatccgctcacaatgtcaattgttatccgctcacaatt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z