LOCUS       BBa_K242002              885 bp    DNA     linear       05-Apr-2025
DEFINITION  P2 and P4 transactivators (cox & ogr) IPTG inducible
ACCESSION   BBa_K242002
VERSION     BBa_K242002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..224
                     /label=ogr native rbs
     CDS             225..443
                     /label=ogr orf
     misc_feature    441..446
                     /label=double translation stop
     CDS             209..446
                     /label=BBa_K242000
     RBS             455..469
                     /label=native rbs for cox
     misc_feature    743..748
                     /label=double terminator
     misc_feature    470..745
                     /label=cox orf
     CDS             455..748
                     /label=BBa_K242001
     stem_loop       768..811
                     /label=stem_loop
     misc_feature    757..836
                     /label=BBa_B0010
     terminator      757..836
                     /label=BBa_B0010
     stem_loop       852..871
                     /label=T7 TE
     misc_feature    878..878
                     /label=stop
     polyA_site      872..885
                     /label=polya
     misc_feature    845..885
                     /label=BBa_B0012
     terminator      845..885
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg aagaggtgct cgcgatgttt cattgtcctt
      241 tatgccagca tgccgcacat gcgcgtacaa gtcgctatat cactgacacg acaaaagagc
      301 gttatcatca gtgccagaac gtgaattgca gcgccacgtt catcacttat gagtcggtac
      361 agcgatacat cgtgaagccg ggagaagtcc acgccgtaag gccgcacccg ttgccatcag
      421 ggcagcaaat tatgtggatg taataatact agaggggcta gaggacgaca tgagcaagca
      481 agtaacactc atgactgatg cgattcctta tcaggagttc gcaaaactaa taggaaaatc
      541 gacaggagcg gttcgtcgga tgatcgataa aggaaagctg cctgtaattg atatgaccga
      601 tccacaatca gcttcaggtc gtgcaggtga atattgggta taccttccgg catggaataa
      661 cggactaaaa ctggcttatg aaagccgccc taaagagatt cgtgacggct ggttgatgtg
      721 gttaggtctc ggtgaaccac gttaataata ctagagccag gcatcaaata aaacgaaagg
      781 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      841 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//