BBa_K242051 1 BBa_K242051 Essential region of P4 bacteriophage genome 2009-10-19T11:00:00Z 2015-05-08T01:11:37Z This sequence comes from P4 bacteriophage genome from positions (0 to 18)+(4090 to 11624) http://www.ncbi.nlm.nih.gov/nuccore/9627507?report=graph&from=1&to=9087 Bacteriophage P4 genome without the non-essential region nor the integrase gene. The use of this part is similar to a plasmid. You can clone standard parts in this viral vector. You can use this vector with a system for P4 production to encapsidate your constructions and transduce them. false false _340_ 0 3074 9 Not in stock true We remove all forbiden restriction sites, (EcoRI,XbaI,SpeI,PstI), from P4 genome but it means remove the integrase also. For our project it was useful because removing the integrase gene we expect P4 to be maintained as a plasmid. However it could mean a reduction in the stability of the plasmid without selection. false Enrique Paz annotation2057348 1 cos site range2057348 1 7507 7560 BBa_K242051_sequence 1 ccggactcaaacatcaaaataagttaatgataaaaaacaaataataaaacacaacaatgaaatatgcccccttttgtgcccccactgtttttctgaccaatctattttcagcccatcaataaatcggaaagttaaatcatttttaatcagtaagtttggatccgtagctcggatccaaaccagtgcatcttttatccacataaaaaattttttttcgaaagaactgttcacactgttcacctttctgttttctccttttatttcagagtgataggtggtgaataatgggtgaagggtgaacattcgattcttcacctccggcattctgccgatgtgactcataccggtgattaatcctccgcactgaaatcactcaggaagaaaaaagttttttttgatttgattgttcacactgttcacctttcgtttttctcttttaatttcagtgtgataacgggtgaatatacggtgaagggtgaacagtggattgttcaccttcgggggatatcgggataaaaaaagaccggcagatgccggtcaggtgggtcaggctgttgtagggtcgtcacattttggcagccagtcgccgtagctttcctctttcagcgtcaggttggtctgtatcccctgtttggtatggcgtttctcgtaattcagtccgtattccttcagcatcaccggcagccccagcccgaacattttcagactgagtacattccggtagccgtttgcctccatgtaggccagataggcgtgatagaggtatttacggtaattgcgcgggatgatactggcgttccccatatacatgccgctggtctgcggcagggtttccagatagccgataaaatcaaacgtcgggtcggcatcccgtttgatgttcagtgcctcgtctgagttctgctgggactgaagcagtgaccgggcgagcatcgggtcgctgaacttctgcatcaggtgacgcacgatgaccgccagctcgcgggtgattttgtccttaagctgcgggtcgcgctcctgcggggctatctgttccgggaagtgaataatcacccgtcggcgtgacacgccgccgctgcggtcggtgaagcgcatcgggttattgttcacggccagaatcaccgccgggatgtgcgtggagtacgcatcccggtatttcgggtcaacggacaccgcatcgccgccggtgatggccttgagtccggcaccgtcgccgctccatttttcctggtccggcaggcgtatcagtgagaagccagttaacgcggcacgttcacgcggggattccagcgtctcgatggtggccgacgtggcgttatcctccccggccagcagggtggctatttcggccatgatacttttgccgctgccgccgggaccggtcacctccagaaagagctgccagtcgtagcggtttgccagcaccataaacagtgcagccagaatcacgtcgcgtttttccgcacggccaccggcggcacggtcaagccagcgccagaaggcgggggcgtgggtttccagcgtttcaccgtccaccggcggggtgaaatccacatcgcacagggtgcgcatccagtgtgacggactgtgcgggtggaacgtgccgttctgcgtgtcgagcacgccgttacgaaagccaatcaggcggcgggagggggcttcctgctgcggaataatcagcttcagggtatccaccacggaggccaccttcccggaggagaacggcgcacgcagacgctgaaacagcccggccacatcccgggcaaagtcctgtggcggcagcaccttccagacaccattttcatagcgggacagaagctggccgttggcatcgaccgcgagcgcctcgccgtaatgctcatagatacgcatggccttttcgctggtactcatggcggaaaactccgcttcgctcatggtgtcgaacgggctttcagccggtggccggatggcatcgtaaatggccttacgggtggcctccccgccgtactgcgtgaaggcatcattccagtcaccgaagaccggcggcagggcaacaacaccttcacacgcatctgcggctgcggcggcttttttctggccgtcaccactgaggtcacggtcagcggcaaggacaatctgacaggcggggtgcttctgccgggcaaggctggccagagaaaggaggttcacggaagaaagcgccaccatcaccgtttcaccggtcaggtgatgtacggtaagtgcggtcgcgtatccctccgctatccacagacgttttccggcctgattctgtccttcaagggtgtgacaggtgcccctgacctgtccgcctttcagggtgcgcttacggccgtcagcactgattaactgaaggttaaccagttcgccgctgtcgtcatacagtggcaccacaaggtcaccggcgcgccagctcacgccaccggctctgtgtgtgccggtcagcatccggcattcccggccgggaaagcccttgcgggtcaggtaggcgttaccggttccggtacgggttttcgccatcagggtttgtgccagtgcggcggcgttcttccgggcagcgtctgtttcatcaacggcggcggtcgtcactgccgggtcagccggtggcaggctgccggtcacggcagccacctttgcggccgcgtcggacggggaaacaccaaaaaccttttcaaccagtttcaggccgtcaccggcaccacactgattgcagtaccaggtgccgcgcccctccctgtcatcaaaacggaagcggtcactcccgccacagaccggacagggctgatgacggttcttcagcacctgaatccccagcgccgggagaatacgcggccagtggccgagcgcatggctgacggtggcggttacgttcattttcatggtgttgttctccttcagtgcagtaccggcgcttttatgtgacgggcacagagttcatccatcacaaccagcccgagaaaggacagcgacggcgcggccttcagggggccggattccattaaatcttccagcagggcacaggctatctgacgccctttttcctcaccgtgctggcgcagataaaagccttccagctcagcggcgatggccgcctccagtgactcaagggtgagatgcgggtagcggtgctgacgttcgcacacggtcagccaggcacaggcgacagcgcgacggtaaagggcagcgcgtaagacgggcggtaagggtgttttcatttgcttttctccctgtgacagatgactgcattccgtgccggttgcattaactgataaggcatatctgcgtctcctgaagacgtgcgtatccctgcgcgaatacgcacatttaatttttcgggggtcgttttttaattacagataattgcggtaactgttatccggggtggtttccgggtcaggctccgtgcggggaatttcccgccattcccgcgccaccggtgctgcccggctgaccggaacagtgtcctgcgggtaaatatccagatatttttcccgccatttctgtaattccgggtctccggccatttctttcagtaccgcatgccggtttacggggctgcgtttaaacaggtcaggacggtcacaggtaaattcccgcagaaaacgccccagcgggatgtctgtggtgcgtccgtcagcgaggatacgcacaaggatactgaatttacggcggtacgggttccagacaatgtccgggcagcggtacggcatttcccacggaataccgtcttccagaatgccgaccacggccacatcgggaaaaccggcagaacggtaaatctcaccgggctggggaaaatcaaacatgcgtcctgtctccccggtctttctgctgggcgagaaaatcgcggcacaggcctttggctttcagctcattcagcacaaaatcaatatcttcattcaggtagctgaaaatatgtggaatgtagagctgatgcaggccggagagttcacggtgaatcaaatcacccccaacaaaccgggatacggcgctggcgcggttgagcttatggtaagcctcaatgctgaggtgttcacgggcgtcatgacgcgctgagacggtctgaggggcttttttattacgcacgggacacctccaccaccggcagacgggcagcaagggagagcacatagtcacggacaagggaacggcgggcactgcgttcatcaccggcgacggtgcgaagcatacagatacggggatgacggtctgcgcgacggacagccgcaaacacaaagacaaattcagggtgtgagggggtaagggttgtagccatgatggcagcctcctgtgaatagcaaataacgctatcgccggagttctcacgctcgatggcgatagcccagacgggggtgagaataccggcttcacaggataccggccagcccggaggctgccccgcctgagctaccattgactctgcggcataatgagcggacgcgggcaggatgcacggaatgccatctgcacgactgaccacacaccacaccataatctggcgctctgtggcattgattgcgacacaaaaaaagacgcgtggcgcgtcatatgtcgcctgtgaattgctcgggttctcacgcccggctgccgattttgcggcaggcgaaaaactatatccgcaaatgccggaaaaaggcaagccagaaaaagggagtttttgcagagcgggcatcatcatgcgtcgtacccccgtttgcgtccggcaatgcgtccggccatccatgcggtgacttcagagtgcagccaggccacatttttaccgccaagactcacctgcggcggaaattcccccttacggatgagttcgtagatggtcgagcgtgacaggccgcacaggtgcatcacttccggcacacgtaaaaaacgctcctgcgtgatgtccggcagcggcatcagtggcgtcactggggcgggagacggggaagaaaaaacagcttgcatcgggctacctcgttaatgtccatacagcaccggataagtccgtccggcttcgggtagcgctttattttgtgaatattttcagcagacgcaacaggggggatttgttcaggctgtcttacaatggctgtgtgttttttgttcatctccacttaaagtcatttaaagccacttaaagcaatttgtaatttttatagtgaaatacaaatcgtttcttcttattcattcccggcgaattaataaaaacaaacagtagtaaacagcacaaaaagcccatcaacgggtgaacagtggtgaacagacggtgaacagtcattactgcgattgttcaccctttaacttactgtattacttatcttttttattaaggtgaacagaggtgaacagtaaaatataaaaaaacaaacagtaagccggtttttcctgcgaccttttcctggcttgccggtctgaggatgagtctcctgtgtcagggctggcacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttcttaacctgaagtgacgaggagccggaaaatgtctgaccacactatccctgaatatctgcaacccgcactggcacaactggaaaaggccagagccgcccatcttgagaacgcccgcctgatggatgagaccgtcacggccattgaacgggcagagcaggaaaaaaatgcgctggcgcaggccgacggaaacgacgctgacgactggcgcacggcctttcgtgcagccggtggtgtcctgagcgacgagctgaaacagcgccacattgagcgcgtggcacgccgggagctggtacaggaatatgacaatctggccgtggtgctgaatttcgaacgtgaacgcctgaaaggggcgtgtgacagcacggccaccgcctaccggaaggcacatcatcaccttctgagtctgtatgcagagcatgagctggaacacgccctgaatgaaacctgtgaggcgcttgtccgggcaatgcatctgagcattctggtacaggaaaatccgctcgccaacaccaccggccatcagggctacgtcgcaccggaaaaggctgtcatgcagcaggtgaaatcatcgctggaacagaaaattaaacagatgcaaatcagcctcaccggcgagccggttctccggctgaccggactgtcagcggcaacactcccgcacatggattatgaggtggcaggcacaccggcacagcgcaaggtgtggcaggacaaaatagaccagcagggagcagagcttaaggccagagggctgctgtcatgatttactgtccgtcgtgtggacatgttgctcacacccgtcgcgcacatttcatggacgatggcaccaagataatgattgcacagtgccggaatatttattgctctgcgacatttgaagcgagtgaaagctttttctctgacagtaaagattcaggaatggaatacatttcaggcaaacagagataccgcgattcactgacgtcagcctcctgcggtatgaaacgcccgaaaagaatgcttgttaccggatattgttgtcggagatgtaaaggccttgcactgtcaagaacatcgcggcgtctgtctcaggaagtcaccgagcgtttttatgtgtgcacggatccgggctgtggtctggtgtttaaaacgcttcagaccatcaaccgcttcattgtccgcccggtcacgccggacgaactggcagaacgcctgcatgaaaaacaggaactgccgccagtacggttaaaaacacaatcatattcgctgcgtctggaatgagggctgccggttaacaccggccgtcgccgcacaccgtatttttattcttcagcatgatgagaaagagataacgatggaaagcacagccttacagcaggcctttgacacctgtcagaataacaaagcagcatggctgcaacgcaaaaatgagctggcagcggccgaacaggaatatctgcggcttctgtcaggagaaggcagaaacgtcagtcgcctggacgaattacgcaatattatcgaagtcagaaaatggcaggtgaatcaggccgccggtcgttatattcgttcgcatgaagccgttcagcacatcagcatccgcgaccggctgaatgattttatgcagcagcacggcacagcactggcggccgcactggcaccggagctgatgggctacagtgagctgacggccattgcccgaaactgtgccatacagcgtgccacagatgccctgcgtgaagcccttctgtcctggcttgcgaagggtgaaaaaattaattattccgcacaggatagcgacattttaacgaccatcggattcaggcctgacgtggcttcggtggatgacagccgtgaaaaattcacccctgcgcagaacatgattttttcgcgtaaaagtgcgcaactggcatcacgtcagtcagtgtaaaattccccgaaaatccgcccgtttttactgaaaaaagccatgcatcgataaggtgcatggctttgcatgcgttttcctgcctcattttctgcaaaccgcgccattcccggcgcggtctgagcgtgtcagtgcaactgcattaaaaccgccccgcaaagcgggcgggcgaggcggggaaagcaccgcgcgcaaaccgacaagttagttaattatttgtgtagtcaaagtgccttcagtacatacctcgttaatacattggagcataatgaagaaaatctatgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z