LOCUS       BBa_K242052             2181 bp    DNA     linear       15-Jun-2024
DEFINITION  RFP+Kanamycin+Ter
ACCESSION   BBa_K242052
VERSION     BBa_K242052.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     misc_feature    1..1069
                     /label=BBa_J04450
     CDS             1226..2044
                     /label=kanamycin resistance
     misc_feature    1078..2044
                     /label=BBa_P1003
     stem_loop       2064..2107
                     /label=stem_loop
     misc_feature    2053..2132
                     /label=BBa_B0010
     terminator      2053..2132
                     /label=BBa_B0010
     stem_loop       2148..2167
                     /label=T7 TE
     misc_feature    2174..2174
                     /label=stop
     polyA_site      2168..2181
                     /label=polya
     misc_feature    2141..2181
                     /label=BBa_B0012
     terminator      2141..2181
                     /label=BBa_B0012
     terminator      2053..2181
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagctg
     1081 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct
     1141 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1201 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcccg
     1261 tccgcgctta aactccaaca tggacgctga tttatatggg tataaatggg ctcgcgataa
     1321 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     1381 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtccgtct
     1441 caactggctg acggagttta tgcctctccc gaccatcaag cattttatcc gtactcctga
     1501 tgatgcgtgg ttactcacca ccgcgattcc tgggaaaaca gccttccagg tattagaaga
     1561 atatcctgat tcaggtgaaa atattgttga tgcgctggcc gtgttcctgc gccggttaca
     1621 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgt gtatttcgtc ttgctcaggc
     1681 gcaatcacgc atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     1741 ctggcctgtt gaacaagtct ggaaagaaat gcacaagctc ttgccattct caccggattc
     1801 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     1861 aggttgtatt gatgttggac gggtcggaat cgcagaccgt taccaggacc ttgccattct
     1921 ttggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     1981 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     2041 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2101 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2161 gtgggccttt ctgcgtttat a
//