LOCUS BBa_K242150 2738 bp DNA linear 22-Nov-2024 DEFINITION (no promoter - AHL) + (ptet - luxR) + (quorum sensing inducible RFP) ACCESSION BBa_K242150 VERSION BBa_K242150.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 598..629 /label=LVA CDS 19..597 /label=luxI misc_feature 19..636 /label=BBa_C0061 misc_feature 637..661 /label=Help:Barcodes CDS 19..636 /label=BBa_C0061 misc_feature 1..661 /label=BBa_C0261 stem_loop 681..724 /label=stem_loop misc_feature 670..749 /label=BBa_B0010 terminator 670..749 /label=BBa_B0010 stem_loop 765..784 /label=T7 TE misc_feature 791..791 /label=stop polyA_site 785..798 /label=polya misc_feature 758..798 /label=BBa_B0012 terminator 758..798 /label=BBa_B0012 terminator 670..798 /label=BBa_B0015 stem_loop 1688..1731 /label=stem_loop misc_feature 1677..1756 /label=BBa_B0010 terminator 1677..1756 /label=BBa_B0010 stem_loop 1772..1791 /label=T7 TE misc_feature 1798..1798 /label=stop polyA_site 1792..1805 /label=polya misc_feature 1765..1805 /label=BBa_B0012 terminator 1765..1805 /label=BBa_B0012 terminator 1677..1805 /label=BBa_B0015 misc_feature 807..825 /label=TetR 1 misc_feature 807..860 /label=BBa_R0040 promoter 826..831 /label=-35 misc_feature 832..850 /label=TetR 2 promoter 849..854 /label=-10 promoter 807..860 /label=BBa_R0040 RBS 869..881 /label=RBS-3\Weak RBS 875..878 /label=RBS misc_feature 869..881 /label=BBa_B0032 RBS 869..881 /label=BBa_B0032 misc_feature 888..889 /label=prefix misc_feature 1061..1061 /label=T misc_feature 1379..1379 /label=A CDS 888..1637 /label=luxR misc_feature 888..1643 /label=BBa_C0062 misc_feature 1644..1668 /label=Help:Barcodes CDS 888..1643 /label=BBa_C0062 misc_feature 869..1668 /label=BBa_K116616 stem_loop 1688..1731 /label=stem_loop misc_feature 1677..1756 /label=BBa_B0010 terminator 1677..1756 /label=BBa_B0010 stem_loop 1772..1791 /label=T7 TE misc_feature 1798..1798 /label=stop polyA_site 1792..1805 /label=polya misc_feature 1765..1805 /label=BBa_B0012 terminator 1765..1805 /label=BBa_B0012 terminator 1677..1805 /label=BBa_B0015 misc_feature 869..1805 /label=BBa_K116626 misc_feature 807..1805 /label=BBa_K116640 misc_feature 1814..1833 /label=LuxR/HSL promoter 1855..1861 /label=-10 misc_feature 1867..1867 /label=start promoter 1833..1838 /label=-35 misc_feature 1814..1869 /label=BBa_R1062 promoter 1814..1869 /label=BBa_R1062 misc_feature 1882..1885 /label=conserved RBS 1878..1889 /label=BBa_B0034 CDS 1896..2570 /label=mrfp1 misc_feature 2577..2601 /label=Help:Barcodes CDS 1896..2576 /label=BBa_E1010 stem_loop 2621..2664 /label=stem_loop misc_feature 2610..2689 /label=BBa_B0010 terminator 2610..2689 /label=BBa_B0010 stem_loop 2705..2724 /label=T7 TE misc_feature 2731..2731 /label=stop polyA_site 2725..2738 /label=polya misc_feature 2698..2738 /label=BBa_B0012 terminator 2698..2738 /label=BBa_B0012 misc_feature 1878..2738 /label=BBa_I13507 ORIGIN 1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 661 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 721 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 781 ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca 841 gtgatagaga tactgagcac tactagagtc acacaggaaa gtactagatg aaaaacataa 901 atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc aataatgata 961 ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat ttactcgcga 1021 tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat taccctaaaa 1081 aatggaggca atattatgat gacgctaatt taataaaata tgatcctata gtagattatt 1141 ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct gtaaataaaa 1201 aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg tttagtttcc 1261 ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca gaaaaagaca 1321 actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt gttccttctc 1381 tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac gatttaacca 1441 aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa 1501 aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg caaatgaaac 1561 tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga gcaattgatt 1621 gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta ctagagccag 1681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1801 ttatatacta gagacctgta ggatcgtaca ggttgacaca agaaaatggt ttgttgatac 1861 tcgaataaat actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca 1921 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa 1981 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta 2041 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt 2101 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg 2161 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc 2221 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact 2281 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac 2341 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag 2401 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc 2461 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca 2521 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct 2581 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc 2641 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 2701 cactggctca ccttcgggtg ggcctttctg cgtttata //