##gff-version 3
##sequence-region BBa_K242150 1 2738
BBa_K242150	.	terminator	670	798	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K242150	.	terminator	670	749	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K242150	.	stem_loop	681	724	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242150	.	engineered_region	670	749	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242150	.	terminator	758	798	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K242150	.	engineered_region	758	798	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242150	.	stop_codon	791	791	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242150	.	stem_loop	765	784	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242150	.	polyA_site	785	798	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242150	.	engineered_region	1	661	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K242150	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K242150	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K242150	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K242150	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K242150	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K242150	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K242150	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K242150	.	terminator	1677	1805	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K242150	.	terminator	1677	1756	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K242150	.	stem_loop	1688	1731	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242150	.	engineered_region	1677	1756	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242150	.	terminator	1765	1805	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K242150	.	engineered_region	1765	1805	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242150	.	stop_codon	1798	1798	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242150	.	stem_loop	1772	1791	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242150	.	polyA_site	1792	1805	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242150	.	engineered_region	1878	2738	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K242150	.	terminator	2698	2738	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K242150	.	engineered_region	2698	2738	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242150	.	stop_codon	2731	2731	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242150	.	stem_loop	2705	2724	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242150	.	polyA_site	2725	2738	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242150	.	ribosome_entry_site	1878	1889	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K242150	.	conserved_region	1882	1885	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K242150	.	CDS	1896	2576	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K242150	.	CDS	1896	2570	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K242150	.	engineered_tag	2577	2601	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K242150	.	terminator	2610	2689	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K242150	.	stem_loop	2621	2664	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242150	.	engineered_region	2610	2689	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242150	.	engineered_region	807	1805	.	+	0	ID=BBa_K116640;Name=BBa_K116640
BBa_K242150	.	promoter	807	860	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K116640
BBa_K242150	.	promoter	826	831	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K242150	.	non_covalent_binding_site	832	850	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K242150	.	engineered_region	807	860	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K242150	.	non_covalent_binding_site	807	825	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K242150	.	promoter	849	854	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K242150	.	engineered_region	869	1805	.	+	0	ID=BBa_K116626;Name=BBa_K116626;Parent=BBa_K116640
BBa_K242150	.	engineered_region	869	1668	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116626
BBa_K242150	.	CDS	888	1643	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K242150	.	CDS	888	1637	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K242150	.	engineered_region	888	1643	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K242150	.	engineered_tag	1644	1668	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K242150	.	point_mutation	1061	1061	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K242150	.	engineered_region	888	889	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K242150	.	point_mutation	1379	1379	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K242150	.	ribosome_entry_site	869	881	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K242150	.	ribosome_entry_site	875	878	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K242150	.	engineered_region	869	881	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K242150	.	ribosome_entry_site	869	881	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K242150	.	terminator	1677	1805	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116626
BBa_K242150	.	terminator	1677	1756	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K242150	.	stem_loop	1688	1731	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242150	.	engineered_region	1677	1756	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242150	.	terminator	1765	1805	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K242150	.	engineered_region	1765	1805	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242150	.	stop_codon	1798	1798	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242150	.	stem_loop	1772	1791	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242150	.	polyA_site	1792	1805	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242150	.	promoter	1814	1869	.	+	0	ID=BBa_R1062;Name=BBa_R1062
BBa_K242150	.	promoter	1833	1838	.	+	0	ID=annotation2101;Name=-35;Parent=BBa_R1062
BBa_K242150	.	engineered_region	1814	1869	.	+	0	ID=annotation7077;Name=BBa_R1062;Parent=BBa_R1062
BBa_K242150	.	start_codon	1867	1867	.	+	0	ID=annotation2100;Name=start;Parent=BBa_R1062
BBa_K242150	.	promoter	1855	1861	.	+	0	ID=annotation2099;Name=-10;Parent=BBa_R1062
BBa_K242150	.	non_covalent_binding_site	1814	1833	.	+	0	ID=annotation2098;Name=LuxR/HSL;Parent=BBa_R1062
>BBa_K242150
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagt
cacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacct
gtaggatcgtacaggttgacacaagaaaatggtttgttgatactcgaataaatactagagaaagaggagaaatactaga
tggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt
cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccg
ctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcc
cggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttac
cgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaag
gtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaa
accggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgtt
gaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata