LOCUS BBa_K242151 2946 bp DNA linear 22-Dec-2024 DEFINITION lacI promoter + lux I + TER + ptet-luxR-TER + lux pR - RFP ACCESSION BBa_K242151 VERSION BBa_K242151.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 806..837 /label=LVA CDS 227..805 /label=luxI misc_feature 227..844 /label=BBa_C0061 misc_feature 845..869 /label=Help:Barcodes CDS 227..844 /label=BBa_C0061 misc_feature 209..869 /label=BBa_C0261 stem_loop 889..932 /label=stem_loop misc_feature 878..957 /label=BBa_B0010 terminator 878..957 /label=BBa_B0010 stem_loop 973..992 /label=T7 TE misc_feature 999..999 /label=stop polyA_site 993..1006 /label=polya misc_feature 966..1006 /label=BBa_B0012 terminator 966..1006 /label=BBa_B0012 terminator 878..1006 /label=BBa_B0015 stem_loop 1896..1939 /label=stem_loop misc_feature 1885..1964 /label=BBa_B0010 terminator 1885..1964 /label=BBa_B0010 stem_loop 1980..1999 /label=T7 TE misc_feature 2006..2006 /label=stop polyA_site 2000..2013 /label=polya misc_feature 1973..2013 /label=BBa_B0012 terminator 1973..2013 /label=BBa_B0012 terminator 1885..2013 /label=BBa_B0015 misc_feature 1015..1033 /label=TetR 1 misc_feature 1015..1068 /label=BBa_R0040 promoter 1034..1039 /label=-35 misc_feature 1040..1058 /label=TetR 2 promoter 1057..1062 /label=-10 promoter 1015..1068 /label=BBa_R0040 RBS 1077..1089 /label=RBS-3\Weak RBS 1083..1086 /label=RBS misc_feature 1077..1089 /label=BBa_B0032 RBS 1077..1089 /label=BBa_B0032 misc_feature 1096..1097 /label=prefix misc_feature 1269..1269 /label=T misc_feature 1587..1587 /label=A CDS 1096..1845 /label=luxR misc_feature 1096..1851 /label=BBa_C0062 misc_feature 1852..1876 /label=Help:Barcodes CDS 1096..1851 /label=BBa_C0062 misc_feature 1077..1876 /label=BBa_K116616 stem_loop 1896..1939 /label=stem_loop misc_feature 1885..1964 /label=BBa_B0010 terminator 1885..1964 /label=BBa_B0010 stem_loop 1980..1999 /label=T7 TE misc_feature 2006..2006 /label=stop polyA_site 2000..2013 /label=polya misc_feature 1973..2013 /label=BBa_B0012 terminator 1973..2013 /label=BBa_B0012 terminator 1885..2013 /label=BBa_B0015 misc_feature 1077..2013 /label=BBa_K116626 misc_feature 1015..2013 /label=BBa_K116640 misc_feature 2022..2041 /label=LuxR/HSL promoter 2063..2069 /label=-10 misc_feature 2075..2075 /label=start promoter 2041..2046 /label=-35 misc_feature 2022..2077 /label=BBa_R1062 promoter 2022..2077 /label=BBa_R1062 misc_feature 2090..2093 /label=conserved RBS 2086..2097 /label=BBa_B0034 CDS 2104..2778 /label=mrfp1 misc_feature 2785..2809 /label=Help:Barcodes CDS 2104..2784 /label=BBa_E1010 stem_loop 2829..2872 /label=stem_loop misc_feature 2818..2897 /label=BBa_B0010 terminator 2818..2897 /label=BBa_B0010 stem_loop 2913..2932 /label=T7 TE misc_feature 2939..2939 /label=stop polyA_site 2933..2946 /label=polya misc_feature 2906..2946 /label=BBa_B0012 terminator 2906..2946 /label=BBa_B0012 misc_feature 2086..2946 /label=BBa_I13507 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat 241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg 301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc 361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt 421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc 481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt 541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact 601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac 661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga 721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga 781 acagtttaaa aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta 841 ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat aaaacgaaag 901 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 961 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta 1021 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagtcac 1081 acaggaaagt actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa 1141 attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg 1201 gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt taaatctgat 1261 atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta 1321 ataaaatatg atcctatagt agattattct aactccaatc attcaccaat taattggaat 1381 atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca 1441 tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg 1501 cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt 1561 atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca 1621 aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc 1681 gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact 1741 ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct 1801 aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata acactgatag 1861 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 1921 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 1981 gctcaccttc gggtgggcct ttctgcgttt atatactaga gacctgtagg atcgtacagg 2041 ttgacacaag aaaatggttt gttgatactc gaataaatac tagagaaaga ggagaaatac 2101 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa 2161 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa 2221 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac 2281 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc 2341 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc 2401 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc 2461 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa 2521 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt 2581 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc 2641 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg 2701 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt 2761 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca 2821 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2881 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2941 tttata //