##gff-version 3
##sequence-region BBa_K242151 1 2946
BBa_K242151	.	terminator	878	1006	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K242151	.	terminator	878	957	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K242151	.	stem_loop	889	932	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242151	.	engineered_region	878	957	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242151	.	terminator	966	1006	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K242151	.	engineered_region	966	1006	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242151	.	stop_codon	999	999	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242151	.	stem_loop	973	992	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242151	.	polyA_site	993	1006	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242151	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K242151	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K242151	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K242151	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K242151	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K242151	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K242151	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K242151	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K242151	.	engineered_region	209	869	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K242151	.	CDS	227	844	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K242151	.	engineered_region	227	844	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K242151	.	tag	806	837	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K242151	.	engineered_tag	845	869	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K242151	.	CDS	227	805	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K242151	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K242151	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K242151	.	promoter	2022	2077	.	+	0	ID=BBa_R1062;Name=BBa_R1062
BBa_K242151	.	promoter	2041	2046	.	+	0	ID=annotation2101;Name=-35;Parent=BBa_R1062
BBa_K242151	.	engineered_region	2022	2077	.	+	0	ID=annotation7077;Name=BBa_R1062;Parent=BBa_R1062
BBa_K242151	.	start_codon	2075	2075	.	+	0	ID=annotation2100;Name=start;Parent=BBa_R1062
BBa_K242151	.	promoter	2063	2069	.	+	0	ID=annotation2099;Name=-10;Parent=BBa_R1062
BBa_K242151	.	non_covalent_binding_site	2022	2041	.	+	0	ID=annotation2098;Name=LuxR/HSL;Parent=BBa_R1062
BBa_K242151	.	engineered_region	2086	2946	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K242151	.	terminator	2906	2946	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K242151	.	engineered_region	2906	2946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242151	.	stop_codon	2939	2939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242151	.	stem_loop	2913	2932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242151	.	polyA_site	2933	2946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242151	.	ribosome_entry_site	2086	2097	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K242151	.	conserved_region	2090	2093	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K242151	.	CDS	2104	2784	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K242151	.	CDS	2104	2778	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K242151	.	engineered_tag	2785	2809	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K242151	.	terminator	2818	2897	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K242151	.	stem_loop	2829	2872	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242151	.	engineered_region	2818	2897	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242151	.	terminator	1885	2013	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K242151	.	terminator	1885	1964	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K242151	.	stem_loop	1896	1939	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242151	.	engineered_region	1885	1964	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242151	.	terminator	1973	2013	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K242151	.	engineered_region	1973	2013	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242151	.	stop_codon	2006	2006	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242151	.	stem_loop	1980	1999	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242151	.	polyA_site	2000	2013	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242151	.	engineered_region	1015	2013	.	+	0	ID=BBa_K116640;Name=BBa_K116640
BBa_K242151	.	promoter	1015	1068	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K116640
BBa_K242151	.	promoter	1034	1039	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K242151	.	non_covalent_binding_site	1040	1058	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K242151	.	engineered_region	1015	1068	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K242151	.	non_covalent_binding_site	1015	1033	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K242151	.	promoter	1057	1062	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K242151	.	engineered_region	1077	2013	.	+	0	ID=BBa_K116626;Name=BBa_K116626;Parent=BBa_K116640
BBa_K242151	.	engineered_region	1077	1876	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116626
BBa_K242151	.	CDS	1096	1851	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K242151	.	CDS	1096	1845	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K242151	.	engineered_region	1096	1851	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K242151	.	engineered_tag	1852	1876	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K242151	.	point_mutation	1269	1269	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K242151	.	engineered_region	1096	1097	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K242151	.	point_mutation	1587	1587	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K242151	.	ribosome_entry_site	1077	1089	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K242151	.	ribosome_entry_site	1083	1086	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K242151	.	engineered_region	1077	1089	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K242151	.	ribosome_entry_site	1077	1089	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K242151	.	terminator	1885	2013	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116626
BBa_K242151	.	terminator	1885	1964	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K242151	.	stem_loop	1896	1939	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242151	.	engineered_region	1885	1964	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242151	.	terminator	1973	2013	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K242151	.	engineered_region	1973	2013	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242151	.	stop_codon	2006	2006	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242151	.	stem_loop	1980	1999	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242151	.	polyA_site	2000	2013	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K242151
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgactataat
gataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaag
caaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatattt
atgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgt
ttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaa
aatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtc
aaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcg
tattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaa
aaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtga
tagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgaaaaacat
aaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatct
gatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatattt
caatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtaga
ttattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgta
attaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgc
ttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgt
tccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaa
gaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtca
ctttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacagg
agcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttgacacaagaaaa
tggtttgttgatactcgaataaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagag
ttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtc
cgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcccc
gcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaa
ggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacg
gtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatggg
ttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaa
gacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaa
ccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca
ctccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata