LOCUS       BBa_K242900             2437 bp    DNA     linear       14-Nov-2024
DEFINITION  [p(T3/T7)-GFP-(E3 + E9 toxins)]
ACCESSION   BBa_K242900
VERSION     BBa_K242900.1  
FEATURES             Location/Qualifiers
     promoter        4..26
                     /label=T7 RNA polymerase promoter
     promoter        30..45
                     /label=T3 RNA polymerase promoter
     RBS             55..66
                     /label=rbs
     promoter        1..76
                     /label=BBa_K242100
     misc_feature    83..841
                     /label=BBa_E0044
     CDS             1..841
                     /label=BBa_K242101
     RBS             852..862
                     /label=RBS
     CDS             868..1186
                     /label=E3 RNase domain
     misc_feature    1187..1192
                     /label=stop
     CDS             850..1192
                     /label=BBa_K242200
     RBS             1201..1212
                     /label=rbs
     misc_feature    1221..1223
                     /label=start
     CDS             1221..1625
                     /label=E9 DNase Domain
     misc_feature    1626..1631
                     /label=stop
     CDS             1201..1631
                     /label=BBa_K242201
     misc_feature    1644..1647
                     /label=conserved
     RBS             1640..1651
                     /label=BBa_B0034
     misc_feature    2237..2268
                     /label=LVA
     CDS             1658..2236
                     /label=luxI
     misc_feature    1658..2275
                     /label=BBa_C0061
     misc_feature    2276..2300
                     /label=Help:Barcodes
     CDS             1658..2275
                     /label=BBa_C0061
     misc_feature    1640..2300
                     /label=BBa_C0261
     stem_loop       2320..2363
                     /label=stem_loop
     misc_feature    2309..2388
                     /label=BBa_B0010
     terminator      2309..2388
                     /label=BBa_B0010
     stem_loop       2404..2423
                     /label=T7 TE
     misc_feature    2430..2430
                     /label=stop
     polyA_site      2424..2437
                     /label=polya
     misc_feature    2397..2437
                     /label=BBa_B0012
     terminator      2397..2437
                     /label=BBa_B0012
     terminator      2309..2437
                     /label=BBa_B0015
ORIGIN
        1 tactaatacg actcactata gggagaaata ccctcactaa agggactcgt gcagaaagag
       61 gagaaatact agaatgtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      121 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      181 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      241 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      301 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      421 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      481 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      541 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      601 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      661 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      721 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
      781 ggatgaacta tacaaaaggc ctgctgcaaa cgacgaaaac tacgctgctg ctgtttaata
      841 atactagagg aaagaggaga aatactagat gaacgatgaa aagaataagc ccagaaaagg
      901 ttttaaagat tacgggcatg attatcatcc agctccgaaa actgagaata ttaaagggct
      961 tggtgatctt aagcctggga taccaaaaac accaaagcag aatggtggtg gaaaacgcaa
     1021 gcgctggact ggagataaag ggcgtaagat ttatgagtgg gattctcagc atggtgagct
     1081 tgaggggtat cgtgccagtg atggtcagca tcttggctca tttgacccta aaacaggcaa
     1141 tcagttgaaa ggtccagatc cgaaacgaaa tatcaagaaa tatctttaat aatactagag
     1201 aaagaggaga aatactagag atgaaggaga gtaaacggaa taagccaggg aaggcgacag
     1261 gtaaaggtaa accagttggt gataaatggc tggatgatgc aggtaaagat tcaggagcgc
     1321 caattccaga tcgcattgct gataagttgc gtgataaaga atttaaaagc ttcgacgatt
     1381 ttcggaaggc tgtatgggaa gaggtgtcga aagatcctga gcttagtaaa aatttaaacc
     1441 caagcaataa gtctagtgtt tcaaaaggtt attctccgtt tactccaaag aatcaacagg
     1501 tcggagggag aaaagtctat gaacttcatc atgacaagcc aattagtcaa ggtggtgagg
     1561 tttatgacat ggataatatc cgagtgacta cacctaagcg acatatcgat attcaccgag
     1621 gtaagtaata atactagaga aagaggagaa atactagatg actataatga taaaaaaatc
     1681 ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc gttatcaagt
     1741 gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat cagatgagta
     1801 tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg taagtggatg
     1861 ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc ctgaattgct
     1921 tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt ttgctgtagg
     1981 taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac tatttgaagc
     2041 tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa catcaacagc
     2101 aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag acaaagaaat
     2161 tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg aacagtttaa
     2221 aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt aataactctg
     2281 atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2341 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2401 actggctcac cttcgggtgg gcctttctgc gtttata
//