LOCUS BBa_K242901 5544 bp DNA linear 25-Nov-2024 DEFINITION (pT3/T7-GFP-E3-E9-LuxI-T)+(pTet-LuxR-T)+(plux-RFP)+(plux-antisenseT 3/T7-T) ACCESSION BBa_K242901 VERSION BBa_K242901.1 FEATURES Location/Qualifiers promoter 4..26 /label=T7 RNA polymerase promoter promoter 30..45 /label=T3 RNA polymerase promoter RBS 55..66 /label=rbs promoter 1..76 /label=BBa_K242100 misc_feature 83..841 /label=BBa_E0044 CDS 1..841 /label=BBa_K242101 RBS 852..862 /label=RBS CDS 868..1186 /label=E3 RNase domain misc_feature 1187..1192 /label=stop CDS 850..1192 /label=BBa_K242200 RBS 1201..1212 /label=rbs misc_feature 1221..1223 /label=start CDS 1221..1625 /label=E9 DNase Domain misc_feature 1626..1631 /label=stop CDS 1201..1631 /label=BBa_K242201 misc_feature 1644..1647 /label=conserved RBS 1640..1651 /label=BBa_B0034 misc_feature 2237..2268 /label=LVA CDS 1658..2236 /label=luxI misc_feature 1658..2275 /label=BBa_C0061 misc_feature 2276..2300 /label=Help:Barcodes CDS 1658..2275 /label=BBa_C0061 misc_feature 1640..2300 /label=BBa_C0261 stem_loop 2320..2363 /label=stem_loop misc_feature 2309..2388 /label=BBa_B0010 terminator 2309..2388 /label=BBa_B0010 stem_loop 2404..2423 /label=T7 TE misc_feature 2430..2430 /label=stop polyA_site 2424..2437 /label=polya misc_feature 2397..2437 /label=BBa_B0012 terminator 2397..2437 /label=BBa_B0012 terminator 2309..2437 /label=BBa_B0015 misc_feature 1..2437 /label=BBa_K242900 misc_feature 2450..2453 /label=conserved RBS 2446..2457 /label=BBa_B0034 CDS 2464..3138 /label=mrfp1 misc_feature 3145..3169 /label=Help:Barcodes CDS 2464..3169 /label=BBa_E1010 stem_loop 3189..3232 /label=stem_loop misc_feature 3178..3257 /label=BBa_B0010 terminator 3178..3257 /label=BBa_B0010 stem_loop 3273..3292 /label=T7 TE misc_feature 3299..3299 /label=stop polyA_site 3293..3306 /label=polya misc_feature 3266..3306 /label=BBa_B0012 terminator 3266..3306 /label=BBa_B0012 misc_feature 2446..3306 /label=BBa_K094130 misc_feature 3315..3333 /label=TetR 1 misc_feature 3315..3368 /label=BBa_R0040 promoter 3334..3339 /label=-35 misc_feature 3340..3358 /label=TetR 2 promoter 3357..3362 /label=-10 promoter 3315..3368 /label=BBa_R0040 RBS 3377..3389 /label=RBS-3\Weak RBS 3383..3386 /label=RBS misc_feature 3377..3389 /label=BBa_B0032 RBS 3377..3389 /label=BBa_B0032 misc_feature 3396..3397 /label=prefix misc_feature 3569..3569 /label=T misc_feature 3887..3887 /label=A CDS 3396..4145 /label=luxR misc_feature 3396..4151 /label=BBa_C0062 misc_feature 4152..4176 /label=Help:Barcodes CDS 3396..4151 /label=BBa_C0062 misc_feature 3377..4176 /label=BBa_K116616 stem_loop 4196..4239 /label=stem_loop misc_feature 4185..4264 /label=BBa_B0010 terminator 4185..4264 /label=BBa_B0010 stem_loop 4280..4299 /label=T7 TE misc_feature 4306..4306 /label=stop polyA_site 4300..4313 /label=polya misc_feature 4273..4313 /label=BBa_B0012 terminator 4273..4313 /label=BBa_B0012 terminator 4185..4313 /label=BBa_B0015 misc_feature 3377..4313 /label=BBa_K116626 misc_feature 3315..4313 /label=BBa_K116640 misc_feature 4326..4329 /label=conserved RBS 4322..4333 /label=BBa_B0034 CDS 4340..5014 /label=mrfp1 misc_feature 5021..5045 /label=Help:Barcodes CDS 4340..5020 /label=BBa_E1010 stem_loop 5065..5108 /label=stem_loop misc_feature 5054..5133 /label=BBa_B0010 terminator 5054..5133 /label=BBa_B0010 stem_loop 5149..5168 /label=T7 TE misc_feature 5175..5175 /label=stop polyA_site 5169..5182 /label=polya misc_feature 5142..5182 /label=BBa_B0012 terminator 5142..5182 /label=BBa_B0012 misc_feature 4322..5182 /label=BBa_I13507 misc_feature 5191..5210 /label=LuxR/HSL promoter 5232..5238 /label=-10 misc_feature 5244..5244 /label=start promoter 5210..5215 /label=-35 misc_feature 5191..5246 /label=BBa_R1062 promoter 5191..5246 /label=BBa_R1062 stem_loop 5427..5470 /label=stem_loop misc_feature 5416..5495 /label=BBa_B0010 terminator 5416..5495 /label=BBa_B0010 stem_loop 5511..5530 /label=T7 TE misc_feature 5537..5537 /label=stop polyA_site 5531..5544 /label=polya misc_feature 5504..5544 /label=BBa_B0012 terminator 5504..5544 /label=BBa_B0012 misc_feature 5191..5544 /label=BBa_K242251 ORIGIN 1 tactaatacg actcactata gggagaaata ccctcactaa agggactcgt gcagaaagag 61 gagaaatact agaatgtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc 121 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg 181 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact 241 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag 301 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt 361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa 421 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga 481 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat 541 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga 601 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt 661 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga 721 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat 781 ggatgaacta tacaaaaggc ctgctgcaaa cgacgaaaac tacgctgctg ctgtttaata 841 atactagagg aaagaggaga aatactagat gaacgatgaa aagaataagc ccagaaaagg 901 ttttaaagat tacgggcatg attatcatcc agctccgaaa actgagaata ttaaagggct 961 tggtgatctt aagcctggga taccaaaaac accaaagcag aatggtggtg gaaaacgcaa 1021 gcgctggact ggagataaag ggcgtaagat ttatgagtgg gattctcagc atggtgagct 1081 tgaggggtat cgtgccagtg atggtcagca tcttggctca tttgacccta aaacaggcaa 1141 tcagttgaaa ggtccagatc cgaaacgaaa tatcaagaaa tatctttaat aatactagag 1201 aaagaggaga aatactagag atgaaggaga gtaaacggaa taagccaggg aaggcgacag 1261 gtaaaggtaa accagttggt gataaatggc tggatgatgc aggtaaagat tcaggagcgc 1321 caattccaga tcgcattgct gataagttgc gtgataaaga atttaaaagc ttcgacgatt 1381 ttcggaaggc tgtatgggaa gaggtgtcga aagatcctga gcttagtaaa aatttaaacc 1441 caagcaataa gtctagtgtt tcaaaaggtt attctccgtt tactccaaag aatcaacagg 1501 tcggagggag aaaagtctat gaacttcatc atgacaagcc aattagtcaa ggtggtgagg 1561 tttatgacat ggataatatc cgagtgacta cacctaagcg acatatcgat attcaccgag 1621 gtaagtaata atactagaga aagaggagaa atactagatg actataatga taaaaaaatc 1681 ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc gttatcaagt 1741 gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat cagatgagta 1801 tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg taagtggatg 1861 ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc ctgaattgct 1921 tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt ttgctgtagg 1981 taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac tatttgaagc 2041 tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa catcaacagc 2101 aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag acaaagaaat 2161 tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg aacagtttaa 2221 aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt aataactctg 2281 atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2341 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2401 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaaaga ggagaaatac 2461 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa 2521 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa 2581 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac 2641 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc 2701 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc 2761 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc 2821 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa 2881 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt 2941 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc 3001 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg 3061 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt 3121 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca 3181 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3241 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3301 tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 3361 ctgagcacta ctagagtcac acaggaaagt actagatgaa aaacataaat gccgacgaca 3421 catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct 3481 tatctgatat gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc 3541 attctatggt taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat 3601 attatgatga cgctaattta ataaaatatg atcctatagt agattattct aactccaatc 3661 attcaccaat taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg 3721 taattaaaga agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg 3781 ctaacaatgg cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata 3841 gtttattttt acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt 3901 atcgaaaaat aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag 3961 aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt 4021 gcagtgagcg tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa 4081 accgctgcca aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta 4141 aaaattaata acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa 4201 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 4261 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 4321 gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt 4381 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga 4441 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct 4501 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa 4561 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga 4621 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca 4681 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc 4741 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga 4801 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga 4861 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa 4921 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta 4981 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag 5041 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 5101 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 5161 ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt tgacacaaga 5221 aaatggtttg ttgatactcg aataaatact agagcctcaa tgtcacttac gagcataatg 5281 ccctcctttg gtttcgtgct aaatgataat cataaaggcc acccatgtaa gcgtaaggtt 5341 cagcggtacc cagcgcagag gtgaaaatct tcttagccat aatgttaatc tcctttaggt 5401 ttcgttttac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 5461 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 5521 cgggtgggcc tttctgcgtt tata //