##gff-version 3
##sequence-region BBa_K242901 1 5544
BBa_K242901	.	engineered_region	4322	5182	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K242901	.	CDS	4340	5020	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K242901	.	engineered_tag	5021	5045	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K242901	.	CDS	4340	5014	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K242901	.	terminator	5054	5133	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K242901	.	engineered_region	5054	5133	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242901	.	stem_loop	5065	5108	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242901	.	ribosome_entry_site	4322	4333	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K242901	.	conserved_region	4326	4329	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K242901	.	terminator	5142	5182	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K242901	.	engineered_region	5142	5182	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242901	.	stop_codon	5175	5175	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242901	.	stem_loop	5149	5168	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242901	.	polyA_site	5169	5182	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242901	.	engineered_region	2446	3306	.	+	0	ID=BBa_K094130;Name=BBa_K094130
BBa_K242901	.	terminator	3178	3257	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K094130
BBa_K242901	.	engineered_region	3178	3257	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242901	.	stem_loop	3189	3232	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242901	.	ribosome_entry_site	2446	2457	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K094130
BBa_K242901	.	conserved_region	2450	2453	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K242901	.	terminator	3266	3306	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K094130
BBa_K242901	.	engineered_region	3266	3306	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242901	.	stop_codon	3299	3299	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242901	.	stem_loop	3273	3292	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242901	.	polyA_site	3293	3306	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242901	.	CDS	2464	3169	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K094130
BBa_K242901	.	engineered_tag	3145	3169	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K242901	.	CDS	2464	3138	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K242901	.	engineered_region	3315	4313	.	+	0	ID=BBa_K116640;Name=BBa_K116640
BBa_K242901	.	engineered_region	3377	4313	.	+	0	ID=BBa_K116626;Name=BBa_K116626;Parent=BBa_K116640
BBa_K242901	.	engineered_region	3377	4176	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116626
BBa_K242901	.	CDS	3396	4151	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K242901	.	point_mutation	3887	3887	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K242901	.	CDS	3396	4145	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K242901	.	engineered_region	3396	4151	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K242901	.	engineered_tag	4152	4176	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K242901	.	point_mutation	3569	3569	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K242901	.	engineered_region	3396	3397	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K242901	.	ribosome_entry_site	3377	3389	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K242901	.	ribosome_entry_site	3383	3386	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K242901	.	ribosome_entry_site	3377	3389	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K242901	.	engineered_region	3377	3389	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K242901	.	terminator	4185	4313	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116626
BBa_K242901	.	terminator	4185	4264	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K242901	.	engineered_region	4185	4264	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242901	.	stem_loop	4196	4239	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242901	.	terminator	4273	4313	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K242901	.	engineered_region	4273	4313	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242901	.	stop_codon	4306	4306	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242901	.	stem_loop	4280	4299	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242901	.	polyA_site	4300	4313	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242901	.	promoter	3315	3368	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K116640
BBa_K242901	.	promoter	3334	3339	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K242901	.	non_covalent_binding_site	3340	3358	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K242901	.	engineered_region	3315	3368	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K242901	.	promoter	3357	3362	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K242901	.	non_covalent_binding_site	3315	3333	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K242901	.	engineered_region	1	2437	.	+	0	ID=BBa_K242900;Name=BBa_K242900
BBa_K242901	.	terminator	2309	2437	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K242900
BBa_K242901	.	terminator	2309	2388	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K242901	.	engineered_region	2309	2388	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242901	.	stem_loop	2320	2363	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242901	.	terminator	2397	2437	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K242901	.	engineered_region	2397	2437	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242901	.	stop_codon	2430	2430	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242901	.	stem_loop	2404	2423	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242901	.	polyA_site	2424	2437	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242901	.	CDS	1	841	.	+	0	ID=BBa_K242101;Name=BBa_K242101;Parent=BBa_K242900
BBa_K242901	.	promoter	1	76	.	+	0	ID=BBa_K242100;Name=BBa_K242100;Parent=BBa_K242101
BBa_K242901	.	promoter	30	45	.	+	0	ID=annotation2057295;Name=T3 RNA polymerase promoter;Parent=BBa_K242100
BBa_K242901	.	promoter	4	26	.	+	0	ID=annotation2057294;Name=T7 RNA polymerase promoter;Parent=BBa_K242100
BBa_K242901	.	ribosome_entry_site	55	66	.	+	0	ID=annotation2057296;Name=rbs;Parent=BBa_K242100
BBa_K242901	.	engineered_region	83	841	.	+	0	ID=BBa_E0044;Name=BBa_E0044;Parent=BBa_K242101
BBa_K242901	.	CDS	850	1192	.	+	0	ID=BBa_K242200;Name=BBa_K242200;Parent=BBa_K242900
BBa_K242901	.	CDS	868	1186	.	+	0	ID=annotation2056254;Name=E3 RNase domain;Parent=BBa_K242200
BBa_K242901	.	ribosome_entry_site	852	862	.	+	0	ID=annotation2056253;Name=RBS;Parent=BBa_K242200
BBa_K242901	.	stop_codon	1187	1192	.	+	0	ID=annotation2056273;Name=stop;Parent=BBa_K242200
BBa_K242901	.	engineered_region	1640	2300	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K242900
BBa_K242901	.	CDS	1658	2275	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K242901	.	CDS	1658	2236	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K242901	.	engineered_tag	2276	2300	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K242901	.	engineered_region	1658	2275	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K242901	.	tag	2237	2268	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K242901	.	ribosome_entry_site	1640	1651	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K242901	.	conserved_region	1644	1647	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K242901	.	CDS	1201	1631	.	+	0	ID=BBa_K242201;Name=BBa_K242201;Parent=BBa_K242900
BBa_K242901	.	CDS	1221	1625	.	+	0	ID=annotation2033945;Name=E9 DNase Domain;Parent=BBa_K242201
BBa_K242901	.	stop_codon	1626	1631	.	+	0	ID=annotation2033946;Name=stop;Parent=BBa_K242201
BBa_K242901	.	start_codon	1221	1223	.	+	0	ID=annotation2033944;Name=start;Parent=BBa_K242201
BBa_K242901	.	ribosome_entry_site	1201	1212	.	+	0	ID=annotation2033943;Name=rbs;Parent=BBa_K242201
BBa_K242901	.	mature_transcript_region	5191	5544	.	+	0	ID=BBa_K242251;Name=BBa_K242251
BBa_K242901	.	terminator	5504	5544	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K242251
BBa_K242901	.	engineered_region	5504	5544	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K242901	.	stop_codon	5537	5537	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K242901	.	stem_loop	5511	5530	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K242901	.	polyA_site	5531	5544	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K242901	.	terminator	5416	5495	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K242251
BBa_K242901	.	engineered_region	5416	5495	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K242901	.	stem_loop	5427	5470	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K242901	.	promoter	5191	5246	.	+	0	ID=BBa_R1062;Name=BBa_R1062;Parent=BBa_K242251
BBa_K242901	.	promoter	5232	5238	.	+	0	ID=annotation2099;Name=-10;Parent=BBa_R1062
BBa_K242901	.	promoter	5210	5215	.	+	0	ID=annotation2101;Name=-35;Parent=BBa_R1062
BBa_K242901	.	non_covalent_binding_site	5191	5210	.	+	0	ID=annotation2098;Name=LuxR/HSL;Parent=BBa_R1062
BBa_K242901	.	start_codon	5244	5244	.	+	0	ID=annotation2100;Name=start;Parent=BBa_R1062
BBa_K242901	.	engineered_region	5191	5246	.	+	0	ID=annotation7077;Name=BBa_R1062;Parent=BBa_R1062
>BBa_K242901
tactaatacgactcactatagggagaaataccctcactaaagggactcgtgcagaaagaggagaaatactagaatgtac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaaaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagaggaaagaggagaaatactaga
tgaacgatgaaaagaataagcccagaaaaggttttaaagattacgggcatgattatcatccagctccgaaaactgagaa
tattaaagggcttggtgatcttaagcctgggataccaaaaacaccaaagcagaatggtggtggaaaacgcaagcgctgg
actggagataaagggcgtaagatttatgagtgggattctcagcatggtgagcttgaggggtatcgtgccagtgatggtc
agcatcttggctcatttgaccctaaaacaggcaatcagttgaaaggtccagatccgaaacgaaatatcaagaaatatct
ttaataatactagagaaagaggagaaatactagagatgaaggagagtaaacggaataagccagggaaggcgacaggtaa
aggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgat
aagttgcgtgataaagaatttaaaagcttcgacgattttcggaaggctgtatgggaagaggtgtcgaaagatcctgagc
ttagtaaaaatttaaacccaagcaataagtctagtgtttcaaaaggttattctccgtttactccaaagaatcaacaggt
cggagggagaaaagtctatgaacttcatcatgacaagccaattagtcaaggtggtgaggtttatgacatggataatatc
cgagtgactacacctaagcgacatatcgatattcaccgaggtaagtaataatactagagaaagaggagaaatactagat
gactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaa
gtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcag
aatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgct
gaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgct
gtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacg
ctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttcc
ttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaa
cagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagt
gtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaag
aggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgt
taacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagtt
accaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaac
acccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaaga
cggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc
aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaag
acggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccac
ctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaa
gactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagt
gtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccc
tatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagat
gaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaa
tgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaat
ctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcc
tatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatct
ccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggct
tcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacc
attaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaa
agagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagc
gtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaat
tttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaagaggagaaatactagatggct
tcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaa
tcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgcc
gttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggac
tacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgtta
cccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtcc
ggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaa
atcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccgg
ttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaaca
gtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttgac
acaagaaaatggtttgttgatactcgaataaatactagagcctcaatgtcacttacgagcataatgccctcctttggtt
tcgtgctaaatgataatcataaaggccacccatgtaagcgtaaggttcagcggtacccagcgcagaggtgaaaatcttc
ttagccataatgttaatctcctttaggtttcgttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata