BBa_K243007
1
SL-Fok_A
Short Linker-Fok_a
2009-10-13T11:00:00Z
2015-05-08T01:11:37Z
Combinated by cloning.
This part is the combination of the parts BBa_K243004(Short Linker) and the part BBa_K243000(the protein domain Foka). These combination allows a direct coupling of the protein domain to another part e.g. a tag for purification. The linker can secure a independent function of the protein domain when it is linked to another part.
false
false
_352_
0
4732
9
It's complicated
false
The length of the linker is important to guarantee the independent function of the protein domain when it is linked two another part.
false
Freiburg Bioware09
component2042072
1
BBa_K243000
component2042068
1
BBa_K243004
component2042070
1
BBa_B0105
annotation2042068
1
BBa_K243004
range2042068
1
1
12
annotation2042072
1
BBa_K243000
range2042072
1
19
597
annotation2042070
1
BBa_B0105
range2042070
1
13
18
BBa_B0105
1
Scar 25
RFC 25 Scar Sequence
2009-10-14T11:00:00Z
2015-08-31T04:07:21Z
BBF RFC 25
This is the scar produced by assembly using RFC 25.
If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
false
false
_1_
0
25
397
Not in stock
false
Simple DNA sequence
false
Randy Rettberg
annotation2041621
1
Scar 25
range2041621
1
1
6
BBa_K243004
1
ShortLinke
Short Linker (Gly-Gly-Ser-Gly)
2009-10-11T11:00:00Z
2015-05-08T01:11:37Z
Oligos synthesized by sigma. Hybridized by PCR.
This linker was used to connect two different parts. The sequence produced the aminoacids Gly-Gly-Ser-Gly.
false
true
_352_
0
4732
9
In stock
false
None.
false
Freiburg Bioware09
annotation2041881
1
Short Linker
range2041881
1
1
12
BBa_K243000
1
Fok_a
Protein domain (active) of the restriction endonuclease FokI
2009-10-07T11:00:00Z
2015-05-08T01:11:37Z
extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene
This part is used as the active domain of our universal restriction endonuclease. It cut double stranded DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001).
false
false
_352_
0
4732
9
It's complicated
true
Modifications of the vector (catalytic active heterodimer)
-heterodimeric aminio acids
* switch Glutamate 490/310-312 to Lysin (GAA->AAA)
* switch isoleucin 538/454-456 to Lysin (ATC->AAA)
false
Freiburg Bioware09
annotation2041869
1
Fok_a
range2041869
1
1
579
BBa_B0105_sequence
1
accggc
BBa_K243004_sequence
1
ggtggttctggt
BBa_K243007_sequence
1
ggtggttctggtaccggcaaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaacctgatggtgccatttataccgttggttccccgatcgattatggcgttatcgttgataccaaagcctatagcgggggttataacctgccaattggtcaggctgatgagatgcagcgttatgtgaaagagaaccagactcgtaacaaacacatcaacccgaacgaatggtggaaagtgtatccgtcaagcgttacagagttcaaattcctgttcgtgagcggccattttaaaggcaactataaagcacagctgacccgtctgaaccataaaaccaatagcaatggcgccgttctgtcagtagaagagctgctgattggcggtgaaatgatcaaagccgggaccctgacactggaagaagttcgccgtaaattcaacaatggggagatcaatttt
BBa_K243000_sequence
1
aaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaacctgatggtgccatttataccgttggttccccgatcgattatggcgttatcgttgataccaaagcctatagcgggggttataacctgccaattggtcaggctgatgagatgcagcgttatgtgaaagagaaccagactcgtaacaaacacatcaacccgaacgaatggtggaaagtgtatccgtcaagcgttacagagttcaaattcctgttcgtgagcggccattttaaaggcaactataaagcacagctgacccgtctgaaccataaaaccaatagcaatggcgccgttctgtcagtagaagagctgctgattggcggtgaaatgatcaaagccgggaccctgacactggaagaagttcgccgtaaattcaacaatggggagatcaatttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z