LOCUS       BBa_K243012             1197 bp    DNA     linear       22-Nov-2024
DEFINITION  Strep-DigA-Middle Linker-Fok_i
ACCESSION   BBa_K243012
VERSION     BBa_K243012.1  
FEATURES             Location/Qualifiers
     CDS             1..24
                     /label=Strep-Tag II
     CDS             1..24
                     /label=Strep-Tag II
     misc_feature    1..24
                     /label=BBa_K157012
     misc_feature    25..30
                     /label=Scar 25
     misc_feature    25..30
                     /label=BBa_B0105
     misc_feature    31..582
                     /label=Digoxigenin tag
     misc_feature    31..582
                     /label=BBa_K243003
     misc_feature    583..588
                     /label=Scar 25
     misc_feature    583..588
                     /label=BBa_B0105
     misc_feature    589..612
                     /label=MiddleLinker
     misc_feature    589..612
                     /label=BBa_K243005
     misc_feature    613..618
                     /label=Scar 25
     misc_feature    613..618
                     /label=BBa_B0105
     CDS             619..1197
                     /label=Fok_i
     misc_feature    619..1197
                     /label=BBa_K243001
ORIGIN
        1 tggagccatc cgcagtttga aaaaaccggc gacgtgtacc acgacggcgc ctgccccgaa
       61 gtgaagcccg tggacaactt cgactggtcc cagtaccacg gcaagtggtg gcaggtggcc
      121 gcttatcccg accacatcac caagtacggc aagtgcggct gggccgagta cacccccgag
      181 ggcaagagcg tgaaggtgtc ccggtacagc gtgatccacg gcaaagagta cttcagcgag
      241 ggcaccgcct accctgtggg cgacagcaag atcggcaaga tctaccacag ctacaccatc
      301 ggcggcgtga cccaggtggg cgtgagcaac gtgctgtcca ccgacaacaa gaactacatc
      361 atcggctact tttgcagata cgacgaggac aagaagggcc actgggacgc cgtgtgggtg
      421 ctgtcccggt ccatggtgct gaccggcgag gccaagaccg ccgtggagaa ctacctgatc
      481 ggcagccccg tggtggacag ccagaaactg gtgtacagcg acttctccga ggccgcctgc
      541 aaagtgaaca acagcaactg gtcccacccc cagttcgaaa agaccggcgg tggttctggt
      601 ggtggttctg gtaccggcaa atctgaactg gaggagaaaa aatccgagct gcgccacaaa
      661 ctgaaatatg tgcctcacga gtatatcgaa ctgatcgaga tcgcccgtaa tagtacccaa
      721 gaccgtatcc tggaaatgaa agtgatggag ttcttcatga aagtctatgg ctatcgtggc
      781 aaacatctgg gtggtagccg taaaccagca ggtgccattt ataccgttgg ttccccgatc
      841 gattatggcg ttatcgtggc cacaaaagcg tattctggcg gttataatct gccgattggt
      901 caggctgatg agatggaacg ttatgtggaa gagaatcaga cccgtaacaa acatctgaac
      961 ccgaacgaat ggtggaaagt gtatccgtca agtgtcaccg agttcaaatt tctgttcgtg
     1021 agcggccact ttaaaggcaa ctataaagcc cagctgactc gtctgaacca tatcaccaat
     1081 agcaatgggg cagtgctgag tgttgaggaa ctgctgatcg gtggagaaat gatcaaagca
     1141 ggcaccctga ctctggaaga agttcgccgt aaattcaaca atggcgagat caatttt
//