BBa_K157012
1
BBa_K157012
Strep affinity tag II; Freiburg standard
2008-10-25T11:00:00Z
2015-05-08T01:10:54Z
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
Strep tag as NgoMIV / AgeI protein fusion part, fusion to proteins facilitates detection, purification, immobilization.
false
false
_232_
0
1673
9
It's complicated
false
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
true
Kristian M??ller
annotation2041866
1
Strep-Tag II
range2041866
1
1
24
annotation2041865
1
Strep-Tag II
range2041865
1
1
24
BBa_K243001
1
Fok_i
Protein domain (inactive) of the restriction endonuclease FokI
2009-10-07T11:00:00Z
2015-05-08T01:11:37Z
extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene
This part is used as the inactive domain of our universal restriction endonuclease. It fused with the active protein domain of our universal restriction endonuclease(BBa_K243000)and linked with specific oligos.
false
false
_352_
0
4732
9
It's complicated
true
Modifications of the vector (catalytic inactive heterodimer)
-heterodimeric amino acids
* switch Glutamin 486/298-300 to Glutamate (CAA->GAA)
* switch Isoleucin 499/337-339 to Leucin (ATC->CTG)
-catalytic amino acids
* switch Aspartate 450/190-192 to Alanin (GAC->GCG)
* switch Aspartate 467/243-245 to Alanin (GAT->GCG)
false
Freiburg Bioware09
annotation2041872
1
Fok_i
range2041872
1
1
579
BBa_K243005
1
MiddleLink
Middle Linker ( Gly-Gly-Ser-Gly)x2
2009-10-13T11:00:00Z
2015-05-08T01:11:37Z
Oligos synthesized by sigma. Hybridized by PCR.
This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly .
false
false
_352_
0
4732
9
It's complicated
false
None.
false
Freiburg Bioware09
annotation2041884
1
MiddleLinker
range2041884
1
1
24
BBa_K243012
1
BBa_K243012
Strep-DigA-Middle Linker-Fok_i
2009-10-13T11:00:00Z
2015-05-08T01:11:37Z
Combined the parts by serial cloning steps.
These part units some of our parts.
false
false
_352_
0
4732
9
It's complicated
false
The choice of the linker, lipocalin tag and purification tag allows us serval different combinations.
false
Freiburg Bioware09
component2042179
1
BBa_K243005
component2042177
1
BBa_B0105
component2042181
1
BBa_B0105
component2042171
1
BBa_K157012
component2042175
1
BBa_K243003
component2042183
1
BBa_K243001
component2042173
1
BBa_B0105
annotation2042177
1
BBa_B0105
range2042177
1
583
588
annotation2042183
1
BBa_K243001
range2042183
1
619
1197
annotation2042173
1
BBa_B0105
range2042173
1
25
30
annotation2042175
1
BBa_K243003
range2042175
1
31
582
annotation2042179
1
BBa_K243005
range2042179
1
589
612
annotation2042181
1
BBa_B0105
range2042181
1
613
618
annotation2042171
1
BBa_K157012
range2042171
1
1
24
BBa_K243003
1
DigA
Digoxigenin binding protein (DigA)
2009-10-11T11:00:00Z
2015-05-08T01:11:37Z
synthesized by purimex
Digoxigenin is a steroid extracted from the plant Digitalis purpurea and D.lanata. Digoxigenin modified
oligonucleotides are widely used as high sensitivity probes for non-radioactive immunoassay s and hybridization
experiments. These DIG-probes are monitored by anti-DIG antibodies. These antibodies only show cross-reactivity with blossoms and leaves of D. spec(sole natural sources for DIG) ??? so they are suitable for analysis of any other biological species. Anti-DIG antibodies are either modified with fluorescent dyes (direct detection) or with an enzyme (indirect detection via enzyme-substrate reaction).
false
false
_352_
0
4732
9
It's complicated
true
none
false
Freiburg Bioware09
annotation2041878
1
Digoxigenin tag
range2041878
1
1
552
BBa_B0105
1
Scar 25
RFC 25 Scar Sequence
2009-10-14T11:00:00Z
2015-08-31T04:07:21Z
BBF RFC 25
This is the scar produced by assembly using RFC 25.
If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
false
false
_1_
0
25
397
Not in stock
false
Simple DNA sequence
false
Randy Rettberg
annotation2041621
1
Scar 25
range2041621
1
1
6
BBa_B0105_sequence
1
accggc
BBa_K157012_sequence
1
tggagccatccgcagtttgaaaaa
BBa_K243005_sequence
1
ggtggttctggtggtggttctggt
BBa_K243001_sequence
1
aaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaaccagcaggtgccatttataccgttggttccccgatcgattatggcgttatcgtggccacaaaagcgtattctggcggttataatctgccgattggtcaggctgatgagatggaacgttatgtggaagagaatcagacccgtaacaaacatctgaacccgaacgaatggtggaaagtgtatccgtcaagtgtcaccgagttcaaatttctgttcgtgagcggccactttaaaggcaactataaagcccagctgactcgtctgaaccatatcaccaatagcaatggggcagtgctgagtgttgaggaactgctgatcggtggagaaatgatcaaagcaggcaccctgactctggaagaagttcgccgtaaattcaacaatggcgagatcaatttt
BBa_K243012_sequence
1
tggagccatccgcagtttgaaaaaaccggcgacgtgtaccacgacggcgcctgccccgaagtgaagcccgtggacaacttcgactggtcccagtaccacggcaagtggtggcaggtggccgcttatcccgaccacatcaccaagtacggcaagtgcggctgggccgagtacacccccgagggcaagagcgtgaaggtgtcccggtacagcgtgatccacggcaaagagtacttcagcgagggcaccgcctaccctgtgggcgacagcaagatcggcaagatctaccacagctacaccatcggcggcgtgacccaggtgggcgtgagcaacgtgctgtccaccgacaacaagaactacatcatcggctacttttgcagatacgacgaggacaagaagggccactgggacgccgtgtgggtgctgtcccggtccatggtgctgaccggcgaggccaagaccgccgtggagaactacctgatcggcagccccgtggtggacagccagaaactggtgtacagcgacttctccgaggccgcctgcaaagtgaacaacagcaactggtcccacccccagttcgaaaagaccggcggtggttctggtggtggttctggtaccggcaaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaaccagcaggtgccatttataccgttggttccccgatcgattatggcgttatcgtggccacaaaagcgtattctggcggttataatctgccgattggtcaggctgatgagatggaacgttatgtggaagagaatcagacccgtaacaaacatctgaacccgaacgaatggtggaaagtgtatccgtcaagtgtcaccgagttcaaatttctgttcgtgagcggccactttaaaggcaactataaagcccagctgactcgtctgaaccatatcaccaatagcaatggggcagtgctgagtgttgaggaactgctgatcggtggagaaatgatcaaagcaggcaccctgactctggaagaagttcgccgtaaattcaacaatggcgagatcaatttt
BBa_K243003_sequence
1
gacgtgtaccacgacggcgcctgccccgaagtgaagcccgtggacaacttcgactggtcccagtaccacggcaagtggtggcaggtggccgcttatcccgaccacatcaccaagtacggcaagtgcggctgggccgagtacacccccgagggcaagagcgtgaaggtgtcccggtacagcgtgatccacggcaaagagtacttcagcgagggcaccgcctaccctgtgggcgacagcaagatcggcaagatctaccacagctacaccatcggcggcgtgacccaggtgggcgtgagcaacgtgctgtccaccgacaacaagaactacatcatcggctacttttgcagatacgacgaggacaagaagggccactgggacgccgtgtgggtgctgtcccggtccatggtgctgaccggcgaggccaagaccgccgtggagaactacctgatcggcagccccgtggtggacagccagaaactggtgtacagcgacttctccgaggccgcctgcaaagtgaacaacagcaactggtcccacccccagttcgaaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z