BBa_K243001
1
Fok_i
Protein domain (inactive) of the restriction endonuclease FokI
2009-10-07T11:00:00Z
2015-05-08T01:11:37Z
extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene
This part is used as the inactive domain of our universal restriction endonuclease. It fused with the active protein domain of our universal restriction endonuclease(BBa_K243000)and linked with specific oligos.
false
false
_352_
0
4732
9
It's complicated
true
Modifications of the vector (catalytic inactive heterodimer)
-heterodimeric amino acids
* switch Glutamin 486/298-300 to Glutamate (CAA->GAA)
* switch Isoleucin 499/337-339 to Leucin (ATC->CTG)
-catalytic amino acids
* switch Aspartate 450/190-192 to Alanin (GAC->GCG)
* switch Aspartate 467/243-245 to Alanin (GAT->GCG)
false
Freiburg Bioware09
annotation2041872
1
Fok_i
range2041872
1
1
579
BBa_K157011
1
His
His affinity tag; Freiburg standard
2008-10-25T11:00:00Z
2015-05-08T01:10:54Z
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008
His-tag; fusion to proteins facilitates detection, purification, immobilization;
NgoMIV / AgeI protein fusion part.
false
true
_232_
0
1673
9
It's complicated
false
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
true
Kristian M??ller
annotation2040626
1
His-Tag
range2040626
1
1
18
BBa_K243006
1
LongLinker
Long Linker (Gly-Gly-Ser-Gly)x3
2009-10-13T11:00:00Z
2015-05-08T01:11:37Z
Oligos synthesized by sigma. Hybridized by PCR.
This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly.
false
false
_352_
0
4732
9
It's complicated
false
None.
false
Freiburg Bioware09
annotation2041937
1
Long Linker
range2041937
1
1
36
BBa_K157004
1
BBa_K157004
Fluoresceine-A-binding
2008-10-25T11:00:00Z
2015-05-08T01:10:54Z
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from pieris brassicae), originally designed by Arne Skerra [see Ref. 1,2].
false
false
_232_
0
1673
9
It's complicated
false
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
false
iGEM Team Freiburg 2008
annotation2040659
1
Lipocalin FluA
range2040659
1
1
522
BBa_K243016
1
BBa_K243016
His-FluA-Long Linker-Fok_i
2009-10-13T11:00:00Z
2015-05-08T01:11:37Z
Combined the parts by serial cloning steps.
These part units some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain foka, make it possible to purifier and detect the function of the protein in one step. The fluorescineA tag allows the measurement by quenching and the coupling to a anticalin linked oligo.
false
false
_352_
0
4732
9
It's complicated
false
The choice of the linker, lipocalin tag and purification tag allows us several different combinations.
false
Freiburg Bioware09
component2042670
1
BBa_B0105
component2042664
1
BBa_K157004
component2042662
1
BBa_B0105
component2042672
1
BBa_K243001
component2042668
1
BBa_K243006
component2042660
1
BBa_K157011
component2042666
1
BBa_B0105
annotation2042670
1
BBa_B0105
range2042670
1
589
594
annotation2042668
1
BBa_K243006
range2042668
1
553
588
annotation2042664
1
BBa_K157004
range2042664
1
25
546
annotation2042662
1
BBa_B0105
range2042662
1
19
24
annotation2042660
1
BBa_K157011
range2042660
1
1
18
annotation2042666
1
BBa_B0105
range2042666
1
547
552
annotation2042672
1
BBa_K243001
range2042672
1
595
1173
BBa_B0105
1
Scar 25
RFC 25 Scar Sequence
2009-10-14T11:00:00Z
2015-08-31T04:07:21Z
BBF RFC 25
This is the scar produced by assembly using RFC 25.
If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
false
false
_1_
0
25
397
Not in stock
false
Simple DNA sequence
false
Randy Rettberg
annotation2041621
1
Scar 25
range2041621
1
1
6
BBa_B0105_sequence
1
accggc
BBa_K243016_sequence
1
catcatcatcatcatcataccggcgacgtgtaccacgacggcgcctgccccgaagtgaagcccgtggacaacttcgactggtcccagtaccacggcaagtggtgggaggtggccaagtaccccagccccaacggcaagtatggcaagtgcggctgggccgagtacacccccgagggcaagagcgtgaaggtgtccagatacgacgtgatccacggcaaagaatacttcatggaaggcaccgcctaccccgtgggcgacagcaagatcggcaagatctaccacagccggaccgtgggcggctacaccagaaagaccgtgttcaacgtgctgtccaccgacaacaagaactacatcatcggctacagctgccgctacgacgaggacaagaagggccactgggaccacgtgtgggtgctgtcccggtccatggtgctgaccggcgaggccaagaccgccgtggagaactacctgatcggcagccccgtggtggacagccagaaactggtgtacagcgacttcagcgaggccgcctgcaaagtgaacaacaccggcggtggttctggtggtggttctggtggtggttctggtaccggcaaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaaccagcaggtgccatttataccgttggttccccgatcgattatggcgttatcgtggccacaaaagcgtattctggcggttataatctgccgattggtcaggctgatgagatggaacgttatgtggaagagaatcagacccgtaacaaacatctgaacccgaacgaatggtggaaagtgtatccgtcaagtgtcaccgagttcaaatttctgttcgtgagcggccactttaaaggcaactataaagcccagctgactcgtctgaaccatatcaccaatagcaatggggcagtgctgagtgttgaggaactgctgatcggtggagaaatgatcaaagcaggcaccctgactctggaagaagttcgccgtaaattcaacaatggcgagatcaatttt
BBa_K243001_sequence
1
aaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaaccagcaggtgccatttataccgttggttccccgatcgattatggcgttatcgtggccacaaaagcgtattctggcggttataatctgccgattggtcaggctgatgagatggaacgttatgtggaagagaatcagacccgtaacaaacatctgaacccgaacgaatggtggaaagtgtatccgtcaagtgtcaccgagttcaaatttctgttcgtgagcggccactttaaaggcaactataaagcccagctgactcgtctgaaccatatcaccaatagcaatggggcagtgctgagtgttgaggaactgctgatcggtggagaaatgatcaaagcaggcaccctgactctggaagaagttcgccgtaaattcaacaatggcgagatcaatttt
BBa_K243006_sequence
1
ggtggttctggtggtggttctggtggtggttctggt
BBa_K157011_sequence
1
catcatcatcatcatcat
BBa_K157004_sequence
1
gacgtgtaccacgacggcgcctgccccgaagtgaagcccgtggacaacttcgactggtcccagtaccacggcaagtggtgggaggtggccaagtaccccagccccaacggcaagtatggcaagtgcggctgggccgagtacacccccgagggcaagagcgtgaaggtgtccagatacgacgtgatccacggcaaagaatacttcatggaaggcaccgcctaccccgtgggcgacagcaagatcggcaagatctaccacagccggaccgtgggcggctacaccagaaagaccgtgttcaacgtgctgtccaccgacaacaagaactacatcatcggctacagctgccgctacgacgaggacaagaagggccactgggaccacgtgtgggtgctgtcccggtccatggtgctgaccggcgaggccaagaccgccgtggagaactacctgatcggcagccccgtggtggacagccagaaactggtgtacagcgacttcagcgaggccgcctgcaaagtgaacaac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z