LOCUS       BBa_K243017             1224 bp    DNA     linear       25-Nov-2024
DEFINITION  Strep-DigA-Split Linker-Fok_a
ACCESSION   BBa_K243017
VERSION     BBa_K243017.1  
FEATURES             Location/Qualifiers
     CDS             1..24
                     /label=Strep-Tag II
     CDS             1..24
                     /label=Strep-Tag II
     misc_feature    1..24
                     /label=BBa_K157012
     misc_feature    25..30
                     /label=Scar 25
     misc_feature    25..30
                     /label=BBa_B0105
     misc_feature    31..582
                     /label=Digoxigenin tag
     misc_feature    31..582
                     /label=BBa_K243003
     misc_feature    583..588
                     /label=Scar 25
     misc_feature    583..588
                     /label=BBa_B0105
     CDS             589..639
                     /label=Split-Fluorophore Linker
     misc_feature    589..639
                     /label=BBa_K157009
     misc_feature    640..645
                     /label=Scar 25
     misc_feature    640..645
                     /label=BBa_B0105
     CDS             646..1224
                     /label=Fok_a
     misc_feature    646..1224
                     /label=BBa_K243000
ORIGIN
        1 tggagccatc cgcagtttga aaaaaccggc gacgtgtacc acgacggcgc ctgccccgaa
       61 gtgaagcccg tggacaactt cgactggtcc cagtaccacg gcaagtggtg gcaggtggcc
      121 gcttatcccg accacatcac caagtacggc aagtgcggct gggccgagta cacccccgag
      181 ggcaagagcg tgaaggtgtc ccggtacagc gtgatccacg gcaaagagta cttcagcgag
      241 ggcaccgcct accctgtggg cgacagcaag atcggcaaga tctaccacag ctacaccatc
      301 ggcggcgtga cccaggtggg cgtgagcaac gtgctgtcca ccgacaacaa gaactacatc
      361 atcggctact tttgcagata cgacgaggac aagaagggcc actgggacgc cgtgtgggtg
      421 ctgtcccggt ccatggtgct gaccggcgag gccaagaccg ccgtggagaa ctacctgatc
      481 ggcagccccg tggtggacag ccagaaactg gtgtacagcg acttctccga ggccgcctgc
      541 aaagtgaaca acagcaactg gtcccacccc cagttcgaaa agaccggccg accagcctgt
      601 aagattccaa atgacctgaa gcagaaagtt atgaatcaca ccggcaaatc tgaactggag
      661 gagaaaaaat ccgagctgcg ccacaaactg aaatatgtgc ctcacgagta tatcgaactg
      721 atcgagatcg cccgtaatag tacccaagac cgtatcctgg aaatgaaagt gatggagttc
      781 ttcatgaaag tctatggcta tcgtggcaaa catctgggtg gtagccgtaa acctgatggt
      841 gccatttata ccgttggttc cccgatcgat tatggcgtta tcgttgatac caaagcctat
      901 agcgggggtt ataacctgcc aattggtcag gctgatgaga tgcagcgtta tgtgaaagag
      961 aaccagactc gtaacaaaca catcaacccg aacgaatggt ggaaagtgta tccgtcaagc
     1021 gttacagagt tcaaattcct gttcgtgagc ggccatttta aaggcaacta taaagcacag
     1081 ctgacccgtc tgaaccataa aaccaatagc aatggcgccg ttctgtcagt agaagagctg
     1141 ctgattggcg gtgaaatgat caaagccggg accctgacac tggaagaagt tcgccgtaaa
     1201 ttcaacaatg gggagatcaa tttt
//