LOCUS       BBa_K243018             1218 bp    DNA     linear       25-Nov-2024
DEFINITION  His-DigA-Split Linker-Fok_i
ACCESSION   BBa_K243018
VERSION     BBa_K243018.1  
FEATURES             Location/Qualifiers
     CDS             1..18
                     /label=His-Tag
     misc_feature    1..18
                     /label=BBa_K157011
     misc_feature    19..24
                     /label=Scar 25
     misc_feature    19..24
                     /label=BBa_B0105
     misc_feature    25..576
                     /label=Digoxigenin tag
     misc_feature    25..576
                     /label=BBa_K243003
     misc_feature    577..582
                     /label=Scar 25
     misc_feature    577..582
                     /label=BBa_B0105
     CDS             583..633
                     /label=Split-Fluorophore Linker
     misc_feature    583..633
                     /label=BBa_K157009
     misc_feature    634..639
                     /label=Scar 25
     misc_feature    634..639
                     /label=BBa_B0105
     CDS             640..1218
                     /label=Fok_i
     misc_feature    640..1218
                     /label=BBa_K243001
ORIGIN
        1 catcatcatc atcatcatac cggcgacgtg taccacgacg gcgcctgccc cgaagtgaag
       61 cccgtggaca acttcgactg gtcccagtac cacggcaagt ggtggcaggt ggccgcttat
      121 cccgaccaca tcaccaagta cggcaagtgc ggctgggccg agtacacccc cgagggcaag
      181 agcgtgaagg tgtcccggta cagcgtgatc cacggcaaag agtacttcag cgagggcacc
      241 gcctaccctg tgggcgacag caagatcggc aagatctacc acagctacac catcggcggc
      301 gtgacccagg tgggcgtgag caacgtgctg tccaccgaca acaagaacta catcatcggc
      361 tacttttgca gatacgacga ggacaagaag ggccactggg acgccgtgtg ggtgctgtcc
      421 cggtccatgg tgctgaccgg cgaggccaag accgccgtgg agaactacct gatcggcagc
      481 cccgtggtgg acagccagaa actggtgtac agcgacttct ccgaggccgc ctgcaaagtg
      541 aacaacagca actggtccca cccccagttc gaaaagaccg gccgaccagc ctgtaagatt
      601 ccaaatgacc tgaagcagaa agttatgaat cacaccggca aatctgaact ggaggagaaa
      661 aaatccgagc tgcgccacaa actgaaatat gtgcctcacg agtatatcga actgatcgag
      721 atcgcccgta atagtaccca agaccgtatc ctggaaatga aagtgatgga gttcttcatg
      781 aaagtctatg gctatcgtgg caaacatctg ggtggtagcc gtaaaccagc aggtgccatt
      841 tataccgttg gttccccgat cgattatggc gttatcgtgg ccacaaaagc gtattctggc
      901 ggttataatc tgccgattgg tcaggctgat gagatggaac gttatgtgga agagaatcag
      961 acccgtaaca aacatctgaa cccgaacgaa tggtggaaag tgtatccgtc aagtgtcacc
     1021 gagttcaaat ttctgttcgt gagcggccac tttaaaggca actataaagc ccagctgact
     1081 cgtctgaacc atatcaccaa tagcaatggg gcagtgctga gtgttgagga actgctgatc
     1141 ggtggagaaa tgatcaaagc aggcaccctg actctggaag aagttcgccg taaattcaac
     1201 aatggcgaga tcaatttt
//