LOCUS       BBa_K243022             1278 bp    DNA     linear       11-Dec-2025
DEFINITION  DsbA-Strep-DigA-Split Linker-Fok_a
ACCESSION   BBa_K243022
VERSION     BBa_K243022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..54
                     /label=Dsba signal sequence
     misc_feature    1..54
                     /label=BBa_K243002
     misc_feature    55..60
                     /label=Scar 25
     misc_feature    55..60
                     /label=BBa_B0105
     CDS             61..84
                     /label=Strep-Tag II
     CDS             61..84
                     /label=Strep-Tag II
     misc_feature    61..84
                     /label=BBa_K157012
     misc_feature    85..90
                     /label=Scar 25
     misc_feature    85..90
                     /label=BBa_B0105
     misc_feature    91..642
                     /label=Digoxigenin tag
     misc_feature    91..642
                     /label=BBa_K243003
     misc_feature    643..648
                     /label=Scar 25
     misc_feature    643..648
                     /label=BBa_B0105
     CDS             649..699
                     /label=Split-Fluorophore Linker
     misc_feature    649..699
                     /label=BBa_K157009
     CDS             700..1278
                     /label=Fok_a
     misc_feature    700..1278
                     /label=BBa_K243000
ORIGIN
        1 aaaaagattt ggctggcgct ggctggttta gttttagcgt ttagcgcatc ggcgaccggc
       61 tggagccatc cgcagtttga aaaaaccggc gacgtgtacc acgacggcgc ctgccccgaa
      121 gtgaagcccg tggacaactt cgactggtcc cagtaccacg gcaagtggtg gcaggtggcc
      181 gcttatcccg accacatcac caagtacggc aagtgcggct gggccgagta cacccccgag
      241 ggcaagagcg tgaaggtgtc ccggtacagc gtgatccacg gcaaagagta cttcagcgag
      301 ggcaccgcct accctgtggg cgacagcaag atcggcaaga tctaccacag ctacaccatc
      361 ggcggcgtga cccaggtggg cgtgagcaac gtgctgtcca ccgacaacaa gaactacatc
      421 atcggctact tttgcagata cgacgaggac aagaagggcc actgggacgc cgtgtgggtg
      481 ctgtcccggt ccatggtgct gaccggcgag gccaagaccg ccgtggagaa ctacctgatc
      541 ggcagccccg tggtggacag ccagaaactg gtgtacagcg acttctccga ggccgcctgc
      601 aaagtgaaca acagcaactg gtcccacccc cagttcgaaa agaccggccg accagcctgt
      661 aagattccaa atgacctgaa gcagaaagtt atgaatcaca aatctgaact ggaggagaaa
      721 aaatccgagc tgcgccacaa actgaaatat gtgcctcacg agtatatcga actgatcgag
      781 atcgcccgta atagtaccca agaccgtatc ctggaaatga aagtgatgga gttcttcatg
      841 aaagtctatg gctatcgtgg caaacatctg ggtggtagcc gtaaacctga tggtgccatt
      901 tataccgttg gttccccgat cgattatggc gttatcgttg ataccaaagc ctatagcggg
      961 ggttataacc tgccaattgg tcaggctgat gagatgcagc gttatgtgaa agagaaccag
     1021 actcgtaaca aacacatcaa cccgaacgaa tggtggaaag tgtatccgtc aagcgttaca
     1081 gagttcaaat tcctgttcgt gagcggccat tttaaaggca actataaagc acagctgacc
     1141 cgtctgaacc ataaaaccaa tagcaatggc gccgttctgt cagtagaaga gctgctgatt
     1201 ggcggtgaaa tgatcaaagc cgggaccctg acactggaag aagttcgccg taaattcaac
     1261 aatggggaga tcaatttt
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