BBa_K243027
1
Fos
His-FOS
2009-10-18T11:00:00Z
2015-05-08T01:11:37Z
Ordered by Mr.gene.
The heterodimeric Fos complex is a transcriptional activator involved in the signal transduction pathway. Via leucin zipper they interact among each other and with their bipartite domain, rich of basic amino acids, they bind DNA (Abate et al., Mol Cell Biol. 1991 July).
false
false
_352_
0
4732
9
It's complicated
false
none.
false
Freiburg Bioware09
annotation2061297
1
Fos
range2061297
1
1
216
BBa_K243000
1
Fok_a
Protein domain (active) of the restriction endonuclease FokI
2009-10-07T11:00:00Z
2015-05-08T01:11:37Z
extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene
This part is used as the active domain of our universal restriction endonuclease. It cut double stranded DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001).
false
false
_352_
0
4732
9
It's complicated
true
Modifications of the vector (catalytic active heterodimer)
-heterodimeric aminio acids
* switch Glutamate 490/310-312 to Lysin (GAA->AAA)
* switch isoleucin 538/454-456 to Lysin (ATC->AAA)
false
Freiburg Bioware09
annotation2041869
1
Fok_a
range2041869
1
1
579
BBa_B0105
1
Scar 25
RFC 25 Scar Sequence
2009-10-14T11:00:00Z
2015-08-31T04:07:21Z
BBF RFC 25
This is the scar produced by assembly using RFC 25.
If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
false
false
_1_
0
25
397
Not in stock
false
Simple DNA sequence
false
Randy Rettberg
annotation2041621
1
Scar 25
range2041621
1
1
6
BBa_K157009
1
linker
Split fluorophore linker; Freiburg standard
2008-10-25T11:00:00Z
2015-05-08T01:10:54Z
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008
Originally, this linker was used for fusion to the N-terminus of the C-terminal half of split fluorophores: Protein interactions can be examined by BiFC[1] using complementary, non-fluorescent fragments of fluorophores[2]. Therefore, it is essential that the C-terminal fragment of the fluorophore is not restricted too much in its mobility. The linker allows orientation and adaption of the C-terminal fragment to the N-terminal fragment of the split fluorophore and, thus, the reassembly of a working fluorescent protein. It has already been used in BiFC assays and is known to serve this purpose well[3]; anyway, another, more flexible linker that could be used instead is our ???GGGGS-linker??? (Part Bba_K157010).References see "Part Design".
false
false
_232_
0
1673
9
It's complicated
false
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
false
iGEM Team Freiburg 2008
annotation2040643
1
Split-Fluorophore Linker
range2040643
1
1
51
BBa_K243028
1
BBa_K243028
FOS-Split Linker-Fok_a
2009-10-18T11:00:00Z
2015-05-08T01:11:37Z
Combined by serial cloning steps.
we fused Splitli-Fok_a with Fos, linked to an His-tag. In a mixture of the expressed protein with Jun and Fok_i and the target DNA simultaneously the dimerization of Fos-Splitli-Fok_a with Jun should lead to the interaction of Fos-Splitli-Fok_a with the DNA. After binding and hence activation of the Fok_i and Fok_a partners, the DNA should be cutted. As a prospect the additional feature of photo-switchability of Fos can be generated, allowing regulation of the enzyme???s activity. Introduction of cysteine residues as reactive sites into Fos and fusion of thiol-reactive cross-linkers to them results in intramolecular cross-linking of the Fos protein. By a photo-switch between cis and trans conformations these cross-linkers can change their end to end distance. Thus isomerization can be used to promote helix folding or unfolding of Fos (Woolley et al., Bioinformatics Advance Access published October 17, 2006).
false
false
_352_
0
4732
9
It's complicated
false
none.
false
Freiburg Bioware09
component2052884
1
BBa_B0105
component2052882
1
BBa_K243027
component2052890
1
BBa_K243000
component2052888
1
BBa_B0105
component2052886
1
BBa_K157009
annotation2052890
1
BBa_K243000
range2052890
1
280
858
annotation2052882
1
BBa_K243027
range2052882
1
1
216
annotation2052888
1
BBa_B0105
range2052888
1
274
279
annotation2052884
1
BBa_B0105
range2052884
1
217
222
annotation2052886
1
BBa_K157009
range2052886
1
223
273
BBa_B0105_sequence
1
accggc
BBa_K243027_sequence
1
catcatcatcatcatcatggatccggagaagaaaaacgtcgtattcgtcgtgaacgtaataaaatggcggcggcgaaaagccgtaatcgtcgtcgtgaactgaccgataccctgcaagcggaaaccgatcagctggaagatgaaaaaagcgcgctgcaaaccgaaattgcgaatctgctgaaagaaaaagaaaaactggaatttattctggcggcg
BBa_K243028_sequence
1
catcatcatcatcatcatggatccggagaagaaaaacgtcgtattcgtcgtgaacgtaataaaatggcggcggcgaaaagccgtaatcgtcgtcgtgaactgaccgataccctgcaagcggaaaccgatcagctggaagatgaaaaaagcgcgctgcaaaccgaaattgcgaatctgctgaaagaaaaagaaaaactggaatttattctggcggcgaccggccgaccagcctgtaagattccaaatgacctgaagcagaaagttatgaatcacaccggcaaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaacctgatggtgccatttataccgttggttccccgatcgattatggcgttatcgttgataccaaagcctatagcgggggttataacctgccaattggtcaggctgatgagatgcagcgttatgtgaaagagaaccagactcgtaacaaacacatcaacccgaacgaatggtggaaagtgtatccgtcaagcgttacagagttcaaattcctgttcgtgagcggccattttaaaggcaactataaagcacagctgacccgtctgaaccataaaaccaatagcaatggcgccgttctgtcagtagaagagctgctgattggcggtgaaatgatcaaagccgggaccctgacactggaagaagttcgccgtaaattcaacaatggggagatcaatttt
BBa_K243000_sequence
1
aaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaacctgatggtgccatttataccgttggttccccgatcgattatggcgttatcgttgataccaaagcctatagcgggggttataacctgccaattggtcaggctgatgagatgcagcgttatgtgaaagagaaccagactcgtaacaaacacatcaacccgaacgaatggtggaaagtgtatccgtcaagcgttacagagttcaaattcctgttcgtgagcggccattttaaaggcaactataaagcacagctgacccgtctgaaccataaaaccaatagcaatggcgccgttctgtcagtagaagagctgctgattggcggtgaaatgatcaaagccgggaccctgacactggaagaagttcgccgtaaattcaacaatggggagatcaatttt
BBa_K157009_sequence
1
cgaccagcctgtaagattccaaatgacctgaagcagaaagttatgaatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z