BBa_I757008
1
BBa_I757008
mVenus-YFP_FusionPart
2007-10-23T11:00:00Z
2015-08-31T04:08:07Z
synthetic DNA from GeneArt
# NgoMIV / AgeI protein fusion part
# iGEM Team Freiburg 2007
false
false
_125_
0
1675
9
Not in stock
false
between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions
false
Freiburg iGEM 2007 team
BBa_K243032
1
Fok_a-Venu
Fok_a-Venus
2009-10-19T11:00:00Z
2015-05-08T01:11:37Z
Combiantion of our prtein domain Fok_a and the mVenus-YFP_FusionPartfrom the Team Freiburg07
This is our protein domain Fok_a from our universal endonuclease, which we linked with an YFP-part.
false
false
_352_
0
4732
9
It's complicated
true
Designed according the RFC25
false
Freiburg Bioware09
component2055454
1
BBa_B0105
component2055452
1
BBa_K243000
component2055455
1
BBa_I757008
annotation2055454
1
BBa_B0105
range2055454
1
580
585
annotation2055452
1
BBa_K243000
range2055452
1
1
579
annotation2055455
1
BBa_I757008
range2055455
1
586
1299
BBa_B0105
1
Scar 25
RFC 25 Scar Sequence
2009-10-14T11:00:00Z
2015-08-31T04:07:21Z
BBF RFC 25
This is the scar produced by assembly using RFC 25.
If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
false
false
_1_
0
25
397
Not in stock
false
Simple DNA sequence
false
Randy Rettberg
annotation2041621
1
Scar 25
range2041621
1
1
6
BBa_K243000
1
Fok_a
Protein domain (active) of the restriction endonuclease FokI
2009-10-07T11:00:00Z
2015-05-08T01:11:37Z
extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene
This part is used as the active domain of our universal restriction endonuclease. It cut double stranded DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001).
false
false
_352_
0
4732
9
It's complicated
true
Modifications of the vector (catalytic active heterodimer)
-heterodimeric aminio acids
* switch Glutamate 490/310-312 to Lysin (GAA->AAA)
* switch isoleucin 538/454-456 to Lysin (ATC->AAA)
false
Freiburg Bioware09
annotation2041869
1
Fok_a
range2041869
1
1
579
BBa_B0105_sequence
1
accggc
BBa_K243000_sequence
1
aaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaacctgatggtgccatttataccgttggttccccgatcgattatggcgttatcgttgataccaaagcctatagcgggggttataacctgccaattggtcaggctgatgagatgcagcgttatgtgaaagagaaccagactcgtaacaaacacatcaacccgaacgaatggtggaaagtgtatccgtcaagcgttacagagttcaaattcctgttcgtgagcggccattttaaaggcaactataaagcacagctgacccgtctgaaccataaaaccaatagcaatggcgccgttctgtcagtagaagagctgctgattggcggtgaaatgatcaaagccgggaccctgacactggaagaagttcgccgtaaattcaacaatggggagatcaatttt
BBa_I757008_sequence
1
atgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaactgatttgcaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgggctatggcctgcaatgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacaacagccataacgtgtatatcaccgcggataaacagaaaaacggcatcaaagcgaactttaaaatccgccacaacattgaagatggcggcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagctaccagagcaaactgagcaaagatccgaacgaaaaacgtgaccatatggtgctgctggaatttgtgaccgcggccggtattacccatggcatggatgaactgtataaa
BBa_K243032_sequence
1
aaatctgaactggaggagaaaaaatccgagctgcgccacaaactgaaatatgtgcctcacgagtatatcgaactgatcgagatcgcccgtaatagtacccaagaccgtatcctggaaatgaaagtgatggagttcttcatgaaagtctatggctatcgtggcaaacatctgggtggtagccgtaaacctgatggtgccatttataccgttggttccccgatcgattatggcgttatcgttgataccaaagcctatagcgggggttataacctgccaattggtcaggctgatgagatgcagcgttatgtgaaagagaaccagactcgtaacaaacacatcaacccgaacgaatggtggaaagtgtatccgtcaagcgttacagagttcaaattcctgttcgtgagcggccattttaaaggcaactataaagcacagctgacccgtctgaaccataaaaccaatagcaatggcgccgttctgtcagtagaagagctgctgattggcggtgaaatgatcaaagccgggaccctgacactggaagaagttcgccgtaaattcaacaatggggagatcaattttaccggcatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaaactgaccctgaaactgatttgcaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgggctatggcctgcaatgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgcaggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggtgaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacaacagccataacgtgtatatcaccgcggataaacagaaaaacggcatcaaagcgaactttaaaatccgccacaacattgaagatggcggcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataaccattatctgagctaccagagcaaactgagcaaagatccgaacgaaaaacgtgaccatatggtgctgctggaatttgtgaccgcggccggtattacccatggcatggatgaactgtataaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z