LOCUS       BBa_K243036             1218 bp    DNA     linear       17-May-2024
DEFINITION  His-Dig-Split Linker-Fok_a
ACCESSION   BBa_K243036
VERSION     BBa_K243036.1  
FEATURES             Location/Qualifiers
     CDS             1..18
                     /label=His-Tag
     misc_feature    1..18
                     /label=BBa_K157011
     misc_feature    19..24
                     /label=Scar 25
     misc_feature    19..24
                     /label=BBa_B0105
     misc_feature    25..576
                     /label=Digoxigenin tag
     misc_feature    25..576
                     /label=BBa_K243003
     misc_feature    577..582
                     /label=Scar 25
     misc_feature    577..582
                     /label=BBa_B0105
     CDS             583..633
                     /label=Split-Fluorophore Linker
     misc_feature    583..633
                     /label=BBa_K157009
     misc_feature    634..639
                     /label=Scar 25
     misc_feature    634..639
                     /label=BBa_B0105
     CDS             640..1218
                     /label=Fok_a
     misc_feature    640..1218
                     /label=BBa_K243000
ORIGIN
        1 catcatcatc atcatcatac cggcgacgtg taccacgacg gcgcctgccc cgaagtgaag
       61 cccgtggaca acttcgactg gtcccagtac cacggcaagt ggtggcaggt ggccgcttat
      121 cccgaccaca tcaccaagta cggcaagtgc ggctgggccg agtacacccc cgagggcaag
      181 agcgtgaagg tgtcccggta cagcgtgatc cacggcaaag agtacttcag cgagggcacc
      241 gcctaccctg tgggcgacag caagatcggc aagatctacc acagctacac catcggcggc
      301 gtgacccagg tgggcgtgag caacgtgctg tccaccgaca acaagaacta catcatcggc
      361 tacttttgca gatacgacga ggacaagaag ggccactggg acgccgtgtg ggtgctgtcc
      421 cggtccatgg tgctgaccgg cgaggccaag accgccgtgg agaactacct gatcggcagc
      481 cccgtggtgg acagccagaa actggtgtac agcgacttct ccgaggccgc ctgcaaagtg
      541 aacaacagca actggtccca cccccagttc gaaaagaccg gccgaccagc ctgtaagatt
      601 ccaaatgacc tgaagcagaa agttatgaat cacaccggca aatctgaact ggaggagaaa
      661 aaatccgagc tgcgccacaa actgaaatat gtgcctcacg agtatatcga actgatcgag
      721 atcgcccgta atagtaccca agaccgtatc ctggaaatga aagtgatgga gttcttcatg
      781 aaagtctatg gctatcgtgg caaacatctg ggtggtagcc gtaaacctga tggtgccatt
      841 tataccgttg gttccccgat cgattatggc gttatcgttg ataccaaagc ctatagcggg
      901 ggttataacc tgccaattgg tcaggctgat gagatgcagc gttatgtgaa agagaaccag
      961 actcgtaaca aacacatcaa cccgaacgaa tggtggaaag tgtatccgtc aagcgttaca
     1021 gagttcaaat tcctgttcgt gagcggccat tttaaaggca actataaagc acagctgacc
     1081 cgtctgaacc ataaaaccaa tagcaatggc gccgttctgt cagtagaaga gctgctgatt
     1141 ggcggtgaaa tgatcaaagc cgggaccctg acactggaag aagttcgccg taaattcaac
     1201 aatggggaga tcaatttt
//