##gff-version 3
##sequence-region BBa_K245004 1 2261
BBa_K245004	.	tag	2226	2243	.	+	0	ID=annotation2021216;Name=His tag
BBa_K245004	.	sequence_feature	2119	2261	.	+	0	ID=annotation2021209;Name=modified prefix
BBa_K245004	.	promoter	2133	2151	.	+	0	ID=annotation2021212;Name=T7 promoter
BBa_K245004	.	sequence_feature	2125	2132	.	+	0	ID=annotation2021211;Name=NotI
BBa_K245004	.	sequence_feature	70	77	.	+	0	ID=annotation2021204;Name=NotI
BBa_K245004	.	sequence_feature	244	863	.	+	0	ID=annotation2021206;Name=pBR233 origin
BBa_K245004	.	sequence_feature	1	82	.	+	0	ID=annotation2021198;Name=modified sufix
BBa_K245004	.	promoter	1920	1948	.	+	0	ID=annotation2021208;Name=amp promoter
BBa_K245004	.	sequence_feature	1	6	.	+	0	ID=annotation2021199;Name=XmaI
BBa_K245004	.	sequence_feature	2119	2124	.	+	0	ID=annotation2021210;Name=EcoRI
BBa_K245004	.	sequence_feature	2244	2249	.	+	0	ID=annotation2021217;Name=XbaI
BBa_K245004	.	stem_loop	22	69	.	+	0	ID=annotation2021203;Name=T7 terminator
BBa_K245004	.	CDS	1018	1878	.	+	0	ID=annotation2021207;Name=amp resistance marker
BBa_K245004	.	start_codon	2220	2222	.	+	0	ID=annotation2021215;Name=start
BBa_K245004	.	stop_codon	19	21	.	+	0	ID=annotation2021202;Name=stop
BBa_K245004	.	sequence_feature	2250	2255	.	+	0	ID=annotation2021218;Name=NgoMIV
BBa_K245004	.	sequence_feature	77	82	.	+	0	ID=annotation2021205;Name=PstI
BBa_K245004	.	ribosome_entry_site	2208	2214	.	+	0	ID=annotation2021214;Name=rbs
BBa_K245004	.	non_covalent_binding_site	2151	2178	.	+	0	ID=annotation2021213;Name=lacO regulatory site
BBa_K245004	.	sequence_feature	13	18	.	+	0	ID=annotation2021201;Name=SpeI
BBa_K245004	.	sequence_feature	7	12	.	+	0	ID=annotation2021200;Name=BspEI
BBa_K245004	.	sequence_feature	2256	2261	.	+	0	ID=annotation2021197;Name=AgeI
>BBa_K245004
cccgggtccggaactagttaactagcataaccccttggggcctctaaacggggctagaggggttttttggcggccgctg
caggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaa
tacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaa
aaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagagg
tggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc
tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatct
cagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatcc
ggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagca
gagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttgg
tatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggt
agcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttcta
cggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcaccta
gatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgc
ttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataa
ctacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt
atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctatt
aattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcg
tggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccat
gttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatg
gttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaacca
agtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatag
cagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcc
agttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaa
caggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaata
ttattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg
gttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaata
ggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgct
aatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaagaaggag
gtaaataatgtatcatcaccatcaccatcactctagagccggcaccggt