BBa_K245004 1 BBa_K245004 -- No Description -- 2009-09-15T11:00:00Z 2015-05-08T01:11:38Z The source is BioBrick vector pSB1A2; modified prefix and suffix sequences are of synthetic origin; ccdB originates from part BBa_P1010. BB-NIC-II-HisN originates from BioBrick vector pSB1A2 but contains modified prefix and suffix according to assembly standard 37. T7 promoter is included in prefix and T7 terminator in suffix. There are four new inner restriction sites added in multiple-cloning site (NgoMIV, AgeI-prefix; XmaI/BspEI-suffix) which enable extension of part cloned into pSB1A30 with S/G on N-terminal or T/G on C-terminal; extension of linker and creating a friendly scar during parts fusion. true false _337_ 0 4948 9 Discontinued false BioBrick vector pSB1A2 was first cut with EcoRI/PstI. An insert containing modified prefix and suffix (according to assembly standard 37) was ligated in between. Subsequently a ccdB domain was inserted between AgeI and XmaI restriction sites. false Ota Fekonja annotation2021202 1 stop range2021202 1 19 21 annotation2021207 1 amp resistance marker range2021207 1 1018 1878 annotation2021199 1 XmaI range2021199 1 1 6 annotation2021212 1 T7 promoter range2021212 1 2133 2151 annotation2021209 1 modified prefix range2021209 1 2119 2261 annotation2021216 1 His tag range2021216 1 2226 2243 annotation2021211 1 NotI range2021211 1 2125 2132 annotation2021197 1 AgeI range2021197 1 2256 2261 annotation2021204 1 NotI range2021204 1 70 77 annotation2021205 1 PstI range2021205 1 77 82 annotation2021217 1 XbaI range2021217 1 2244 2249 annotation2021218 1 NgoMIV range2021218 1 2250 2255 annotation2021200 1 BspEI range2021200 1 7 12 annotation2021215 1 start range2021215 1 2220 2222 annotation2021206 1 pBR233 origin range2021206 1 244 863 annotation2021201 1 SpeI range2021201 1 13 18 annotation2021203 1 T7 terminator range2021203 1 22 69 annotation2021198 1 modified sufix range2021198 1 1 82 annotation2021213 1 lacO regulatory site range2021213 1 2151 2178 annotation2021214 1 rbs range2021214 1 2208 2214 annotation2021210 1 EcoRI range2021210 1 2119 2124 annotation2021208 1 amp promoter range2021208 1 1920 1948 BBa_K245004_sequence 1 cccgggtccggaactagttaactagcataaccccttggggcctctaaacggggctagaggggttttttggcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgctaatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaagaaggaggtaaataatgtatcatcaccatcaccatcactctagagccggcaccggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z