BBa_K245005
1
BBa_K245005
BB-NIC-II-HisN
2009-09-23T11:00:00Z
2015-05-08T01:11:38Z
The source is BioBrick vector pSB1A2; modified prefix and suffix sequences are of synthetic origin; ccdB originates from part BBa_P1010.
BB-NIC-II-HisN originates from BioBrick vector pSB1A2 but contains modified prefix and suffix according to assembly standard 37. T7 promoter, RBS and ATG are included in prefix and T7 terminator and STOP codon in suffix. His tag is also inserted between ATG and multiple cloning site in prefix. There are four new inner restriction sites added in multiple-cloning site (NgoMIV and AgeI in prefix; XmaI and BspEI in suffix) which enable extension of part cloned into BB-NIC-II-HisN with SG on N-terminal or TG on C-terminal; extension of linker and creating a friendly scar during parts fusion.
false
false
_337_
0
4964
9
Not in stock
false
BioBrick vector pSB1A2 was first cut with EcoRI/PstI. An insert containing modified prefix and suffix (according to assembly standard 37) was ligated in between. Subsequently a ccdB domain was inserted between AgeI and XmaI restriction sites.
false
Nika Debeljak
annotation2026905
1
NotI
range2026905
1
70
77
annotation2026913
1
T7 promoter
range2026913
1
2133
2151
annotation2026916
1
START
range2026916
1
2220
2222
annotation2026918
1
XbaI
range2026918
1
2244
2249
annotation2026911
1
EcoRI
range2026911
1
2119
2124
annotation2026919
1
NgoMIV
range2026919
1
2250
2255
annotation2026917
1
His tag
range2026917
1
2226
2243
annotation2026901
1
BspEI
range2026901
1
7
12
annotation2026904
1
T7 terminator
range2026904
1
22
69
annotation2026908
1
amp resistance marker
range2026908
1
1018
1878
annotation2026907
1
pBR233 origin
range2026907
1
244
863
annotation2026903
1
STOP
range2026903
1
19
21
annotation2026902
1
SpeI
range2026902
1
13
18
annotation2026920
1
AgeI
range2026920
1
2256
2261
annotation2026900
1
XmaI
range2026900
1
1
6
annotation2026906
1
PstI
range2026906
1
77
82
annotation2026909
1
amp promoter
range2026909
1
1920
1948
annotation2026910
1
modified prefix
range2026910
1
2119
2261
annotation2026914
1
lacO regulatory site
range2026914
1
2151
2178
annotation2026899
1
modified suffix
range2026899
1
1
82
annotation2026915
1
rbs
range2026915
1
2208
2214
annotation2026912
1
NotI
range2026912
1
2125
2132
BBa_K245005_sequence
1
cccgggtccggaactagttaactagcataaccccttggggcctctaaacgggtgtagaggggttttttggcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgctaatacgactcactataggggaattgtgagcggataacaattccccgctagaaataattttgtttaactttaagaaggaggtaaataatgtatcatcaccatcaccatcactctagagccggcaccggt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z