BBa_K245005 1 BBa_K245005 BB-NIC-II-HisN 2009-09-23T11:00:00Z 2015-05-08T01:11:38Z The source is BioBrick vector pSB1A2; modified prefix and suffix sequences are of synthetic origin; ccdB originates from part BBa_P1010. BB-NIC-II-HisN originates from BioBrick vector pSB1A2 but contains modified prefix and suffix according to assembly standard 37. T7 promoter, RBS and ATG are included in prefix and T7 terminator and STOP codon in suffix. His tag is also inserted between ATG and multiple cloning site in prefix. There are four new inner restriction sites added in multiple-cloning site (NgoMIV and AgeI in prefix; XmaI and BspEI in suffix) which enable extension of part cloned into BB-NIC-II-HisN with SG on N-terminal or TG on C-terminal; extension of linker and creating a friendly scar during parts fusion. false false _337_ 0 4964 9 Not in stock false BioBrick vector pSB1A2 was first cut with EcoRI/PstI. An insert containing modified prefix and suffix (according to assembly standard 37) was ligated in between. Subsequently a ccdB domain was inserted between AgeI and XmaI restriction sites. false Nika Debeljak annotation2026905 1 NotI range2026905 1 70 77 annotation2026913 1 T7 promoter range2026913 1 2133 2151 annotation2026916 1 START range2026916 1 2220 2222 annotation2026918 1 XbaI range2026918 1 2244 2249 annotation2026911 1 EcoRI range2026911 1 2119 2124 annotation2026919 1 NgoMIV range2026919 1 2250 2255 annotation2026917 1 His tag range2026917 1 2226 2243 annotation2026901 1 BspEI range2026901 1 7 12 annotation2026904 1 T7 terminator range2026904 1 22 69 annotation2026908 1 amp resistance marker range2026908 1 1018 1878 annotation2026907 1 pBR233 origin range2026907 1 244 863 annotation2026903 1 STOP range2026903 1 19 21 annotation2026902 1 SpeI range2026902 1 13 18 annotation2026920 1 AgeI range2026920 1 2256 2261 annotation2026900 1 XmaI range2026900 1 1 6 annotation2026906 1 PstI range2026906 1 77 82 annotation2026909 1 amp promoter range2026909 1 1920 1948 annotation2026910 1 modified prefix range2026910 1 2119 2261 annotation2026914 1 lacO regulatory site range2026914 1 2151 2178 annotation2026899 1 modified suffix range2026899 1 1 82 annotation2026915 1 rbs range2026915 1 2208 2214 annotation2026912 1 NotI range2026912 1 2125 2132 BBa_K245005_sequence 1 cccgggtccggaactagttaactagcataaccccttggggcctctaaacgggtgtagaggggttttttggcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgctaatacgactcactataggggaattgtgagcggataacaattccccgctagaaataattttgtttaactttaagaaggaggtaaataatgtatcatcaccatcaccatcactctagagccggcaccggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z