BBa_K245006 1 BBa_K245006 BB-NIC-II-NC 2009-09-24T11:00:00Z 2015-05-08T01:11:38Z The basis for BB-NIC-II-NC is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_P1010. This plasmid backbone is the same as BBa_K245004 except that it doesn't contain His tag at all. false false _337_ 0 4964 9 Not in stock false First, vector BB-NIC-II-HisN was cut with EcoRI and XbaI and insert with appropriate part of prefix modified according to standard 37 without His tag was ligated in between. false Nika Debeljak annotation2027024 1 XmaI range2027024 1 1 6 annotation2027037 1 T7 promoter range2027037 1 2133 2151 annotation2027029 1 T7 terminator range2027029 1 22 69 annotation2027038 1 lacO regulatory site range2027038 1 2151 2178 annotation2027032 1 pBR233 origin range2027032 1 244 863 annotation2027025 1 modified suffix range2027025 1 1 82 annotation2027036 1 NotI range2027036 1 2125 2132 annotation2027044 1 modified prefix range2027044 1 2119 2240 annotation2027026 1 BspEI range2027026 1 7 12 annotation2027042 1 NgoMIV range2027042 1 2229 2234 annotation2027027 1 SpeI range2027027 1 13 18 annotation2027040 1 START range2027040 1 2220 2222 annotation2027034 1 amp promoter range2027034 1 1920 1948 annotation2027031 1 PstI range2027031 1 77 82 annotation2027043 1 AgeI range2027043 1 2235 2240 annotation2027041 1 XbaI range2027041 1 2223 2228 annotation2027028 1 STOP range2027028 1 19 21 annotation2027033 1 amp resistance marker range2027033 1 1018 1878 annotation2027039 1 rbs range2027039 1 2208 2214 annotation2027035 1 EcoRI range2027035 1 2119 2124 annotation2027030 1 NotI range2027030 1 70 77 BBa_K245006_sequence 1 cccgggtccggaactagttaactagcataaccccttggggcctctaaacgggtctagaggggttttttggcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgctaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggaggtaaataatgtctagagccggcaccggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z