##gff-version 3
##sequence-region BBa_K245006 1 2240
BBa_K245006	.	sequence_feature	2119	2124	.	+	0	ID=annotation2027035;Name=EcoRI
BBa_K245006	.	sequence_feature	2235	2240	.	+	0	ID=annotation2027043;Name=AgeI
BBa_K245006	.	sequence_feature	13	18	.	+	0	ID=annotation2027027;Name=SpeI
BBa_K245006	.	sequence_feature	70	77	.	+	0	ID=annotation2027030;Name=NotI
BBa_K245006	.	sequence_feature	2125	2132	.	+	0	ID=annotation2027036;Name=NotI
BBa_K245006	.	stem_loop	22	69	.	+	0	ID=annotation2027029;Name=T7 terminator
BBa_K245006	.	promoter	2133	2151	.	+	0	ID=annotation2027037;Name=T7 promoter
BBa_K245006	.	CDS	1018	1878	.	+	0	ID=annotation2027033;Name=amp resistance marker
BBa_K245006	.	sequence_feature	2220	2222	.	+	0	ID=annotation2027040;Name=START
BBa_K245006	.	sequence_feature	77	82	.	+	0	ID=annotation2027031;Name=PstI
BBa_K245006	.	sequence_feature	244	863	.	+	0	ID=annotation2027032;Name=pBR233 origin
BBa_K245006	.	sequence_feature	2223	2228	.	+	0	ID=annotation2027041;Name=XbaI
BBa_K245006	.	stop_codon	19	21	.	+	0	ID=annotation2027028;Name=STOP
BBa_K245006	.	non_covalent_binding_site	2151	2178	.	+	0	ID=annotation2027038;Name=lacO regulatory site
BBa_K245006	.	sequence_feature	7	12	.	+	0	ID=annotation2027026;Name=BspEI
BBa_K245006	.	sequence_feature	1	82	.	+	0	ID=annotation2027025;Name=modified suffix
BBa_K245006	.	sequence_feature	2229	2234	.	+	0	ID=annotation2027042;Name=NgoMIV
BBa_K245006	.	sequence_feature	1	6	.	+	0	ID=annotation2027024;Name=XmaI
BBa_K245006	.	promoter	1920	1948	.	+	0	ID=annotation2027034;Name=amp promoter
BBa_K245006	.	sequence_feature	2119	2240	.	+	0	ID=annotation2027044;Name=modified prefix
BBa_K245006	.	ribosome_entry_site	2208	2214	.	+	0	ID=annotation2027039;Name=rbs
>BBa_K245006
cccgggtccggaactagttaactagcataaccccttggggcctctaaacgggtctagaggggttttttggcggccgctg
caggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaa
tacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaa
aaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagagg
tggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc
tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatct
cagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatcc
ggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagca
gagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttgg
tatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggt
agcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttcta
cggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcaccta
gatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgc
ttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataa
ctacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt
atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctatt
aattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcg
tggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccat
gttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatg
gttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaacca
agtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatag
cagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcc
agttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaa
caggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaata
ttattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg
gttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaata
ggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgct
aatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggag
gtaaataatgtctagagccggcaccggt