##gff-version 3
##sequence-region BBa_K245007 1 2167
BBa_K245007	.	promoter	2088	2106	.	+	0	ID=annotation2027187;Name=T7 promoter
BBa_K245007	.	sequence_feature	2150	2155	.	+	0	ID=annotation2027190;Name=XbaI
BBa_K245007	.	sequence_feature	2156	2161	.	+	0	ID=annotation2027189;Name=NgoMIV
BBa_K245007	.	sequence_feature	13	18	.	+	0	ID=annotation2027047;Name=SpeI
BBa_K245007	.	sequence_feature	2162	2167	.	+	0	ID=annotation2027188;Name=AgeI
BBa_K245007	.	sequence_feature	207	826	.	+	0	ID=annotation2027076;Name=pBR233 origin
BBa_K245007	.	start_codon	2147	2149	.	+	0	ID=annotation2027192;Name=START
BBa_K245007	.	sequence_feature	1	6	.	+	0	ID=annotation2027045;Name=XmaI
BBa_K245007	.	tag	19	36	.	+	0	ID=annotation2027048;Name=His tag
BBa_K245007	.	sequence_feature	40	45	.	+	0	ID=annotation2027050;Name=PstI
BBa_K245007	.	promoter	1883	1911	.	+	0	ID=annotation2027186;Name=amp promoter
BBa_K245007	.	CDS	981	1841	.	+	0	ID=annotation2027077;Name=amp resistance marker
BBa_K245007	.	sequence_feature	7	12	.	+	0	ID=annotation2027046;Name=BspEI
BBa_K245007	.	sequence_feature	2082	2087	.	+	0	ID=annotation2027191;Name=EcoRI
BBa_K245007	.	stop_codon	37	39	.	+	0	ID=annotation2027049;Name=STOP
>BBa_K245007
cccgggtccggaactagtcaccaccaccaccaccactagctgcaggcttcctcgctcactgactcgctgcgctcggtcg
ttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaa
gaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgc
ccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgt
ttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttc
gggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgt
gtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacg
acttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaa
gtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaa
agagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgc
gcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtta
agggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc
taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctat
ttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgc
tgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgc
agaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccag
ttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag
ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccg
atcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgc
catccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttg
ctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttct
tcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatctt
cagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggc
gacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagc
ggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacg
tctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatc
cttagctttcgctaaggatgatttctggaattctaatacgactcactatagggaatacaagctacttgttctttttgca
tactagagccaccatgtctagagccggcaccggt