BBa_K245007 1 BBa_K245007 BB-NIC-II-HisC 2009-09-24T11:00:00Z 2015-05-08T01:11:38Z The basis for BB-NIC-II-HisC is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_P1010. This plasmid backbone is the same as BBa_K245004 except that it contains His tag inserted between multiple-cloning site and stop codon in suffix instead of between start codon and multiple-cloning site in prefix as in case of BBa_K245004. false false _337_ 0 4964 9 Not in stock false Using overlapping primers and PCR ligation a fragment containing modified prefix, ccdB domain and modified suffix with His tag inserted between multiple-cloning site and stop codon was first obtained. The fragment was then ligated in pSB1A2 vector cut with EcoRI and PstI false Nika Debeljak annotation2027188 1 AgeI range2027188 1 2162 2167 annotation2027187 1 T7 promoter range2027187 1 2088 2106 annotation2027047 1 SpeI range2027047 1 13 18 annotation2027189 1 NgoMIV range2027189 1 2156 2161 annotation2027077 1 amp resistance marker range2027077 1 981 1841 annotation2027076 1 pBR233 origin range2027076 1 207 826 annotation2027045 1 XmaI range2027045 1 1 6 annotation2027048 1 His tag range2027048 1 19 36 annotation2027050 1 PstI range2027050 1 40 45 annotation2027192 1 START range2027192 1 2147 2149 annotation2027049 1 STOP range2027049 1 37 39 annotation2027046 1 BspEI range2027046 1 7 12 annotation2027191 1 EcoRI range2027191 1 2082 2087 annotation2027186 1 amp promoter range2027186 1 1883 1911 annotation2027190 1 XbaI range2027190 1 2150 2155 BBa_K245007_sequence 1 cccgggtccggaactagtcaccaccaccaccaccactagctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattctaatacgactcactatagggaatacaagctacttgttctttttgcatactagagccaccatgtctagagccggcaccggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z